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17-313 PP2A Immunoprecipitation Phosphatase Assay Kit

17-313
25 assays  Kit capacity: 25 assays
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      Replacement Information

      Tabela com principais espec.

      Key ApplicationsAnalytes Available
      PAPP2A
      Description
      Catalogue Number17-313
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPP2A Immunoprecipitation Phosphatase Assay Kit
      References
      Product Information
      Components
      • Malachite Green Solution A (Cat.# 20-105)
      • Malachite Green Additive (Cat.# 20-104)
      • Phosphate Standard (Cat.# 20-103)
      • Threonine Phosphopeptide (K-R-pT-I-R-R) (Cat.# 12-219)
      • Anti-PP2A, C subunit, clone 1D6 (Cat.# 05-421)
      • pNPP Ser/Thr Assay Buffer (Cat.# 20-179)
      • Protein A Agarose
      • 96-Well Microtiter Plate
      HS Code3002 15 90
      Quality LevelMQ100
      Applications
      ApplicationPP2A Immunoprecipitation Phosphatase Assay Kit can be used to detect purified PP2A or PP2A from a cellular lysate. It utilizes the Malachite Green & contains components that have been matched to optimize sensitivity & range of detection.
      Key Applications
      • Phosphatase Assay
      Biological Information
      Analytes Available
      • PP2A
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is one of the three catalytic subunits of protein phosphatase 1 (PP1). PP1 is a serine/threonine specific protein phosphatase known to be involved in the regulation of a variety of cellular processes, such as cell division, glycogen metabolism, muscle contractility, protein synthesis, and HIV-1 viral transcription. Increased PP1 activity has been observed in the end stage of heart failure. Studies in both human and mice suggest that PP1 is an important regulator of cardiac function. Mouse studies also suggest that PP1 functions as a suppressor of learning and memory. Three alternatively spliced transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • PPP2CA
      • PP2CA
      • PP2A-alpha
      • PP2Ac
      • RP-C
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P67775 # PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase. Can dephosphorylate SV40 large T antigen and p53. Dephosphorylates SV40 large T antigen, preferentially on serine residues 120, 123, 677, and perhaps 679. The C subunit was most active, followed by the AC form, which was more active than the ABC form, and activity of all three forms was strongly stimulated by manganese, and to a lesser extent by magnesium. Dephosphorylation by the AC form, but not C or ABC form is inhibited by small T antigen.
      COFACTOR: Binds 1 iron ion per subunit (By similarity). & Binds 1 manganese ion per subunit (By similarity).
      SIZE: 309 amino acids; 35594 Da
      SUBUNIT: PP2A consists of a common heterodimeric core enzyme, composed of a 36 kDa catalytic subunit (subunit C) and a 65 kDa constant regulatory subunit (PR65 or subunit A), that associates with a variety of regulatory subunits. Proteins that associate with the core dimer include three families of regulatory subunits B (the R2/B/PR55/B55, R3/B''/PR72/PR130/PR59 and R5/B'/B56 families), the 48 kDa variable regulatory subunit, viral proteins, and cell signaling molecules.
      SUBCELLULAR LOCATION: Cytoplasm.
      PTM: Reversibly methyl esterified on Leu-309. Carboxyl methylation may play a role in holoenzyme assembly. It varies during the cell cycle. & Phosphorylation of either threonine (by autophosphorylation- activated protein kinase) or tyrosine results in inactivation of the phosphatase. Auto-dephosphorylation has been suggested as a mechanism for reactivation.
      SIMILARITY: SwissProt: P67775 ## Belongs to the PPP phosphatase family. PP-1 subfamily.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoprecipitation of purified PP2A (14-111) or PP2A from a cellular lysate. The activity is assessed by dephosphorylation of the phosphopeptide (K-R-pT-I-R-R).
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size25 assays
      Material PackageKit capacity: 25 assays
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      17-313 04053252010927

      Documentation

      PP2A Immunoprecipitation Phosphatase Assay Kit MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      PP2A Immunoprecipitation Phosphatase Assay Kit Certificados de análise

      TítuloNúmero do lote
      PP2A Immunoprecipitation Phosphatase Assay K - DAM1772778 DAM1772778
      PP2A Immunoprecipitation Phosphatase Assay Kit 2482688
      PP2A Immunoprecipitation Phosphatase Assay Kit 2908117
      PP2A Immunoprecipitation Phosphatase Assay Kit 3141202
      PP2A Immunoprecipitation Phosphatase Assay Kit 2989552
      PP2A Immunoprecipitation Phosphatase Assay Kit 3144783
      PP2A Immunoprecipitation Phosphatase Assay Kit 3054920
      PP2A Immunoprecipitation Phosphatase Assay Kit 2947456
      PP2A Immunoprecipitation Phosphatase Assay Kit 3082313
      PP2A Immunoprecipitation Phosphatase Assay Kit 2459553

      Referências

      Visão geral das referênciasAplicaçãoPub Med ID
      Cytoskeletal role in protection of the failing heart by β-adrenergic blockade.
      Cheng, G; Kasiganesan, H; Baicu, CF; Wallenborn, JG; Kuppuswamy, D; Cooper, G
      American journal of physiology. Heart and circulatory physiology  302  H675-87  2012

      Mostrar Resumo
      Immunoprecipitation22081703 22081703
      Protein phosphatase 2A regulates innate immune and proteolytic responses to cigarette smoke exposure in the lung.
      Alison M Wallace,Andrew Hardigan,Patrick Geraghty,Shaneeza Salim,Adam Gaffney,Jincy Thankachen,Leo Arellanos,Jeanine M D'Armiento,Robert F Foronjy
      Toxicological sciences : an official journal of the Society of Toxicology  126  2012

      Mostrar Resumo
      22223484 22223484
      Lead dysregulates serine/threonine protein phosphatases in human neurons.
      Abdur Rahman,Bruce J Brew,Gilles J Guillemin
      Neurochemical research  36  2011

      Mostrar Resumo Texto completo do artigo
      21046238 21046238
      Peptidyl-prolyl isomerase 1 regulates protein phosphatase 2A-mediated topographic phosphorylation of neurofilament proteins.
      P Rudrabhatla, W Albers, HC Pant
      The Journal of neuroscience : the official journal of the Society for Neuroscience  29  14869-80  2009

      Mostrar Resumo
      19940183 19940183
      The adenoviral E4orf6 protein induces atypical apoptosis in response to DNA damage.
      Lori S Hart, David Ornelles, Constantinos Koumenis
      The Journal of biological chemistry  282  6061-7  2007

      Mostrar Resumo
      17172468 17172468
      cAMP counter-regulates insulin-mediated protein phosphatase-2A inactivation in rat skeletal muscle cells
      Begum, N. and Ragolia, L.
      J Biol Chem, 271:31166-71 (1996)  1996

      Immunoprecipitation8940115 8940115
      Characterization and kinetic analysis of the intracellular domain of human protein tyrosine phosphatase beta (HPTP beta) using synthetic phosphopeptides
      Harder, K. W., et al
      Biochem J, 298 ( Pt 2):395-401 (1994)  1994

      Phosphatase Assay8135747 8135747

      FAQ

      PerguntaResposta
      I still have material left to run more assays, but I need another plate. Where can I find a comparable plate?To get a comparable plate, you can purchase a Costar Brand 96-Well Half Area Plate (for Tissue Culture Section).

      Produtos e aplicações relacionadas

      Kit Component

      Número de catálogo Descrição  
      05-421 Anti-PP2A Antibody, C subunit, clone 1D6 Preço e disponibilidade
      20-104 Malachite Green Additive Preço e disponibilidade
      20-105 Malachite Green Solution A Preço e disponibilidade

      Categorias

      Life Science Research > Protein Detection and Quantification > Activity Assays > Phosphatase Assays