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309800 Dihydroethidium - CAS 38483-26-0 - Calbiochem

A cell-permeable, chemically-reduced ethidium derivative.

Ficha de dados de segurança do material (FDSM) ou ficha de segurança do material (FSM), certificado de análise (CA) e certificado de qualidade (CQ), dossiês e outros documentos disponíveis.

Sinônimos: 2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE

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Tabela com principais espec.

CAS #Empirical Formula
38483-26-0C₂₁H₂₁N₃

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      Description
      OverviewA cell-permeable, chemically-reduced ethidium derivative. Commonly used to analyze respiratory burst in phagocytes. Useful fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, whereas after oxidation of this probe to ethidium, it intercalates cell’s DNA, staining the nucleus with a bright red fluorescence.
      Catalogue Number309800
      Brand Family Calbiochem®
      Synonyms2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE
      References
      ReferencesHaugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
      Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
      Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
      Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
      Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
      Perticarari, S., et al. 1991. Cytometry 12, 687.
      Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
      Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.
      Product Information
      CAS number38483-26-0
      ATP CompetitiveN
      FormPink solid
      Hill FormulaC₂₁H₂₁N₃
      Chemical formulaC₂₁H₂₁N₃
      ReversibleN
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetUseful fluorogenic probe for the detection of reactive oxygen species (ROS)
      Purity≥95% by HPLC
      Physicochemical Information
      Cell permeableY
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSSF7935000
      Safety Information
      R PhraseR: 36/37/38

      Irritating to eyes, respiratory system and skin.
      S PhraseS: 26-36

      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Irritant
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      309800-25MG 04055977216806

      Documentation

      Dihydroethidium - CAS 38483-26-0 - Calbiochem MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Dihydroethidium - CAS 38483-26-0 - Calbiochem Certificados de análise

      TítuloNúmero do lote
      309800

      Referências

      Visão geral de referência
      Haugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
      Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
      Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
      Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
      Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
      Perticarari, S., et al. 1991. Cytometry 12, 687.
      Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
      Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.
      Ficha de dados

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision17-June-2008 RFH
      Synonyms2,7-Diamino-10-ethyl-9-phenyl-9,10-dihydrophenanthridine, DHE
      DescriptionA cell-permeable, chemically reduced ethidium derivative that can be used as a fluorogenic probe for the detection of reactive oxygen species (ROS). It undergoes significant oxidation in resting leukocytes, possibly through the uncoupling of mitochondrial oxidative phosphorylation. Cytosolic dihydroethidium displays blue fluorescence, however, following oxidation to ethidium and subsequent intercalation to DNA, a bright red fluorescence is achieved. Often used to determine the level of superoxide anion in cells by flow cytometry.
      FormPink solid
      Intert gas (Yes/No) Packaged under inert gas
      CAS number38483-26-0
      RTECSSF7935000
      Chemical formulaC₂₁H₂₁N₃
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMF or DMSO (20 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot, flush with an inert gas, and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Irritant
      ReferencesHaugen, T.S., et al. 1999. Eur. Respir. J. 14, 1100.
      Mancini, M., et al. 1997. J. Cell Biol. 138, 449.
      Carter, W.O., et al. 1994. J. Leukoc. Biol. 55, 253.
      Cinco, M., et al. 1994. FEMS Microbiol. Lett. 122, 187.
      Perticarari, S., et al. 1994. J. Immunol. Methods 170, 117.
      Perticarari, S., et al. 1991. Cytometry 12, 687.
      Bucana, C.D., et al. 1990. Exp. Cell Res. 190, 69.
      Rothe, G., and Valet, G. 1990. J. Leukoc. Biol. 47, 440.