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391334 BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem

BES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable.

Ficha de dados de segurança do material (FDSM) ou ficha de segurança do material (FSM), certificado de análise (CA) e certificado de qualidade (CQ), dossiês e outros documentos disponíveis.

Sinônimos: N,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid

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CAS #Empirical Formula
10191-18-1C₆H₁₅NO₅S

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      Description
      OverviewBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable. Chen and Okayama described an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The gradual formation of the calcium phosphate-DNA complex and its slow precipitation onto cells appears to be the most critical factor contributing to the high efficiency of stable transformations.
      Absorbance (100 mM, 260 nm):≤0.05.
      Catalogue Number391334
      Brand Family Calbiochem®
      SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
      References
      ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
      Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
      Product Information
      CAS number10191-18-1
      FormWhite solid
      Hill FormulaC₆H₁₅NO₅S
      Chemical formulaC₆H₁₅NO₅S
      PKA7.12 at 20°C
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥99% by titration (dry basis)
      Physicochemical Information
      Heavy metals≤1 ppm
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSKI7363500
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      391334-100GM 07790788058364

      Documentation

      BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      BES, Free Acid, ULTROL® Grade - CAS 10191-18-1 - Calbiochem Certificados de análise

      TítuloNúmero do lote
      391334

      Referências

      Visão geral de referência
      De Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
      Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.
      Ficha de dados

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-July-2008 RFH
      SynonymsN,N-bis-(2-Hydroxyethyl)-2-aminoethanesulfonic Acid
      DescriptionBES buffer is suitable for use in systems where a calcium phosphate-DNA complex formation is desirable (Absorbance: <0.05 at 260 nm, 100 mM). Chen and Okayama describe an improved transfection system for the stable transformation of cells with plasmid DNA using BES buffer. The slow formation appears to be the most critical factor contributing to the high efficiency of stable transformations. Has a pKa of 7.12 at 20°C.
      FormWhite solid
      CAS number10191-18-1
      RTECSKI7363500
      Chemical formulaC₆H₁₅NO₅S
      Purity≥99% by titration (dry basis)
      SolubilityH₂O (1 mg/ml)
      Storage +15°C to +30°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, store in the refrigerator (4°C). Stock solutions are stable for up to 2 weeks at 4°C.
      Toxicity Standard Handling
      ReferencesDe Graeve, K.G., et al. 1994. J. Appl. Bacteriol. 76, 55.
      Chen, C.A., and Okayama, H. 1988. Biotechniques 6, 632.