Millipore Sigma Vibrant Logo

04-281 Anti-phospho-EGFR (Thr669) Antibody, clone 5F10

Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.

Ficha de dados de segurança do material (FDSM) ou ficha de segurança do material (FSM), certificado de análise (CA) e certificado de qualidade (CQ), dossiês e outros documentos disponíveis.
Research Category: Signaling Research Sub-Category: Growth Factors & Receptors Gene Symbol: EGFR, ERBB1, ERBB, mENA UniProt Number: P00533
View Products on Sigmaaldrich.com
04-281
100 µg  
Recuperando preço...
O preço não pôde ser recuperado
Quantidade mínima necessária para ser múltipla de
Maximum Quantity is
Após finalização do pedido Mais informações
Você salvou ()
 
—
Solicitar preço
—
Disponibilidade limitada Disponibilidade limitada
Insira quantidade 
Descontinuado
Quantidades limitadas disponíveis
Disponibilidade a ser confirmada
    Restante: será informado
      Restante: será informado
      Vai informar
      Contatar o serviço de atendimento ao cliente
      Contact Customer Service

      Ofertas especiais

       

      Contatar o serviço de atendimento ao cliente

      Panorama geral

      Replacement Information

      Ofertas especiais

      100% Performance Guaranteed

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWBMPurifiedMonoclonal Antibody
      Description
      Catalogue Number04-281
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-EGFR (Thr669) Antibody, clone 5F10
      Alternate Names
      • ERBB
      • ERBB1
      • epidermal growth factor receptor
      References
      Product Information
      FormatPurified
      HS Code3002 15 90
      Control
      • Includes PMA and pervanadate treated A431 cell lysate as a positive control
      Presentation100 µg of mouse monoclonal IgG1 liquid in 0.01 mg/ml PBS containing 0.09% sodium azide, PEG, and sucrose with 50 % glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-EGFR (Thr669) Antibody, clone 5F10 detects level of phospho-EGFR (Thr669) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated synthetic peptide encompassing the surrounding amino acids of Thr 669 in human EGFR.
      Clone5F10
      HostMouse
      SpecificityRecognizes EGFR when phosphorylated at Threonine 669.
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGFR
      • ERBB1
      • ERBB
      • mENA
      Modifications
      • Phosphorylation
      Purification MethodThiophilic adsoption and size exclusion chromatography
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P00533 # Isoform 2/truncated isoform may act as an antagonist.
      SIZE: 1210 amino acids; 134277 Da
      SUBUNIT: Binds RIPK1. CBL interacts with the autophosphorylated C- terminal tail of the EGF receptor. Part of a complex with ERBB2 and either PIK3C2A or PIK3C2B. The autophosphorylated form interacts with PIK3C2B, maybe indirectly. Interacts with PELP1.
      SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Isoform 2: Secreted.
      TISSUE SPECIFICITY: Expressed in placenta. Isoform 2 is also expressed in ovarian cancers.
      PTM: Phosphorylation of Ser-695 is partial and occurs only if Thr- 693 is phosphorylated. & Monoubiquitinated and polyubiquitinated upon EGF stimulation; which does not affect tyrosine kinase activity or signaling capacity but may play a role in lysosomal targeting. Polyubiquitin linkage is mainly through 'Lys-63', but linkage through 'Lys-48', 'Lys-11' and 'Lys-29' also occur.DISEASE:SwissProt: P00533 # Defects in EGFR are associated with lung cancer [MIM:211980].
      SIMILARITY: SwissProt: P00533 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily. & Contains 1 protein kinase domain.
      MISCELLANEOUS: Binding of EGF to the receptor leads to dimerization, internalization of the EGF-receptor complex, induction of the tyrosine kinase activity, stimulation of cell DNA synthesis, and cell proliferation.
      Molecular Weight180 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by immunoblot.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions 6 months at -20°C from date of shipment
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      04-281 04053252744242

      Documentation

      Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Anti-phospho-EGFR (Thr669) Antibody, clone 5F10 Certificados de análise

      TítuloNúmero do lote
      Anti-phospho-EGFR (Thr669), clone 5F10 (Mouse monoclonal IgG1) 3118673
      Anti-phospho-EGFR (Thr669), clone 5F10 (Mouse monoclonal IgG1) - 2195988 2195988
      Anti-phospho-EGFR (Thr669), clone 5F10 - 0191S0201 0191S0201
      Anti-phospho-EGFR (Thr669), clone 5F10 - 2029610 2029610
      Anti-phospho-EGFR (Thr669), clone 5F10 - 2058318 2058318
      Anti-phospho-EGFR (Thr669), clone 5F10 - 3601699 3601699
      Anti-phospho-EGFR (Thr669), clone 5F10 - 4144899 4144899
      Anti-phospho-EGFR (Thr669), clone 5F10 - 4177425 4177425
      Anti-phospho-EGFR (Thr669), clone 5F10 - DAM1484509 DAM1484509
      Anti-phospho-EGFR (Thr669), clone 5F10 - NG1895478 NG1895478

      Referências

      Visão geral das referênciasPub Med ID
      Cisplatin induces PKB/Akt activation and p38(MAPK) phosphorylation of the EGF receptor
      Winograd-Katz, S E and Levitzki, A
      Oncogene, 25:7381-90 (2006)  2006

      		16785992 16785992
      Mutational removal of the Thr669 and Ser671 phosphorylation sites alters substrate specificity and ligand-induced internalization of the epidermal growth factor receptor
      Heisermann, G J, et al
      J Biol Chem, 265:12820-7 (1990)  1990

      		2115882 2115882
      Epidermal growth factor receptor threonine and serine residues phosphorylated in vivo
      Heisermann, G J and Gill, G N
      J Biol Chem, 263:13152-8 (1988)  1988

      		3138233 3138233

      Produtos e aplicações relacionadas

      Related Products

      Número de catálogo Descrição  
      05-104 Anti-EGFR Antibody, clone LA22 Preço e disponibilidade
      05-1047 Anti-EGFR (cytoplasmic domain) Antibody, clone 8G6.2 Preço e disponibilidade

      Famílias de produtos

      EGFR Antibodies, Kits & Proteins

      Millipore’s EGFR (Epidermal growth factor receptor) antibodies, kits, and proteins are high quality, validated tools for EGFR research. See below for a selection of EGFR and phospho-EGFR products.
      Saiba mais >>

      Categorias

      Life Science Research > Antibodies and Assays > Primary Antibodies