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ABS29 Anti-p38/SAPK2A Antibody

This Anti-p38/SAPK2A Antibody is validated for use in WB for the detection of p38/SAPK2A.

Anti-p38/SAPK2A Antibody: Ficha de dados de segurança do material (FDSM) ou ficha de segurança do material (FSM), certificado de análise (CA) e certificado de qualidade (CQ), dossiês e outros documentos disponíveis.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: MAPK11, PRKM11, SAPK2 UniProt Number: Q16539
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ABS29
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, R, Eq, Rhesus MacaqueWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABS29
      DescriptionAnti-p38/SAPK2A Antibody
      Alternate Names
      • Mitogen-activated protein kinase 14
      • MAP kinase 14
      • MAPK 14
      • Cytokine suppressive anti-inflammatory drug-binding protein
      • CSAID-binding protein
      • CSBP
      • MAP kinase MXI2
      • MAX-interacting protein 2
      • Mitogen-activated protein kinase p38 alpha
      • MAP kinase p38 alpha
      • Stress-activated protein kinase 2a
      • SAPK2a
      Background InformationMitogen-activated protein kinase 14 (UniProt: Q16539; also known as MAP kinase 14, MAPK 14, Cytokine suppressive anti-inflammatory drug-binding protein, CSAID-binding protein, CSBP, MAP kinase MXI2, MAX-interacting protein 2, Mitogen-activated protein kinase p38 alpha, MAP kinase p38 alpha, p38 alpha, Stress-activated protein kinase 2a, SAPK2a) is encoded by the MAPK14 (also known as CSBP, CSBP1, CSBP2, CSPB1, MXI2, SAPK2A) gene (Gene ID: 1432) in human. p38alpha is a serine/threonine kinase that acts as an essential component of the MAP kinase signal transduction pathway. It is one of the four p38 MAPKs that play an important role in the cascades of cellular responses evoked by proinflammatory cytokines or physical stress leading to direct activation of transcription factors. p38 MAPKs phosphorylate a broad range of proteins and it has been estimated that they may have approximately 200 to 300 substrates each. p38alpha is activated by cell stresses such as DNA damage, heat shock, osmotic shock, and pro-inflammatory stimuli. Its activation occurs through dual phosphorylation of Thr-180 and Tyr-182 by either of two dual specificity kinases, MAP2K3/MKK3 or MAP2K6/MKK6, and potentially also MAP2K4/MKK4. When phosphorylated on both Thr-180 and Tyr-182 it is shown to be about 10-20-fold more active than phosphorylation only on Thr-180. Phosphorylation of p38alpha on Tyr-182 alone keeps it in inactive state. The protein kinase domain of p38alpha is localized to amino acids 24-308.
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • Human brain tissue lysate
      PresentationPurified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-p38/SAPK2A Antibody is validated for use in WB for the detection of p38/SAPK2A.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the protein kinase domain of human p38/SAPK2A.
      EpitopeProtein kinase domain
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThis antibody recognizes p38/SAPK2A at the protein kinase domain.
      Species Reactivity
      • Human
      • Mouse
      • Rat
      • Horse
      • Rhesus Macaque
      Species Reactivity NoteDemonstrated to react with Human, Mouse, and Rat. Predicted to react with Equine and Rhesus Macaque based on 100% sequence homology.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation, and development. This kinase is most closely related to p38 MAP kinase, both of which can be activated by proinflammatory cytokines and environmental stress. This kinase is activated through its phosphorylation by MAP kinase kinases (MKKs), preferably by MKK6. Transcription factor ATF2/CREB2 has been shown to be a substrate of this kinase. [provided by RefSeq].
      Gene Symbol
      • MAPK11
      • PRKM11
      • SAPK2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: Kinase involved in a signal transduction pathway that is activated by changes in the osmolarity of the extracellular environment, by cytokines, or by environmental stress. Phosphorylates preferentially transcription factor ATF2.

      CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.

      COFACTOR: Magnesium (By similarity).

      ENZYME REGULATION: Activated by phosphorylation on threonine and tyrosine by MKK6. Inhibited by pyridinyl-imidazole related compounds.

      TISSUE SPECIFICITY: Highest levels in the brain and heart. Also expressed in the placenta, lung, liver, skeletal muscle, kidney and pancreas.

      DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.

      PTM: Dually phosphorylated on Thr-180 and Tyr-182, which activates the enzyme Probable.

      SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.

      Contains 1 protein kinase domain.
      Molecular Weight~41 kDa observed. An uncharacterized band appears at ~35 kDa in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in human brain tissue lysate.

      Western Blot Analysis: 0.1 µg/mL of this antibody detected p38/SAPK2A on 10 µg of human brain tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      ABS29 04053252684500

      Documentation

      Anti-p38/SAPK2A Antibody MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Anti-p38/SAPK2A Antibody Certificados de análise

      TítuloNúmero do lote
      Anti-p38/SAPK2A - 2430553 2430553
      Anti-p38/SAPK2A - 1977626 1977626
      Anti-p38/SAPK2A - 2309284 2309284
      Anti-p38/SAPK2A - 2326986 2326986
      Anti-p38/SAPK2A - 3194674 3194674
      Anti-p38/SAPK2A - 3379921 3379921
      Anti-p38/SAPK2A - NRG1886280 NRG1886280
      Anti-p38/SAPK2A -2490710 2490710
      Anti-p38/SAPK2A -2687799 2687799
      Anti-p38/SAPK2A -2730003 2730003
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