Millipore Sigma Vibrant Logo

AB152 Anti-Tyrosine Hydroxylase Antibody

View Products on Sigmaaldrich.com
AB152
100 µL  
Recuperando preço...
O preço não pôde ser recuperado
Quantidade mínima necessária para ser múltipla de
Maximum Quantity is
Após finalização do pedido Mais informações
Você salvou ()
 
Solicitar preço
Disponibilidade limitada
Disponibilidade limitada
Insira quantidade 
Descontinuado
Quantidades limitadas disponíveis
Disponibilidade a ser confirmada
    Restante: será informado
      Restante: será informado
      Vai informar
      Contatar o serviço de atendimento ao cliente
      Contact Customer Service

      Ofertas especiais

       

      Contatar o serviço de atendimento ao cliente

      Panorama geral

      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, M, Fe, Ft, Sqd, Dr, MlELISA, IF, IHC, IH(P), IP, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB152
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Tyrosine Hydroxylase Antibody
      Alternate Names
      • Tyrosine 3-hydroxylase
      • Tyrosine 3-monooxygenase
      • tyrosine hydroxylase
      • TH
      Background InformationTyrosine hydroxylase (also know as Tyrosine 3-hydroxylase and Tyrosine 3-monooxygenase) plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
      References
      Product Information
      FormatAffinity Purified
      HS Code3002 15 90
      Control
      • POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
        NEGATATIVE CONTROL: Liver.
      PresentationPurified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Tyrosine Hydroxylase Antibody detects level of TH and has been published and validated for use in ELISA, IF, IH, IH(P), IP and WB.
      Key Applications
      • ELISA
      • Immunofluorescence
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunohistochemistry:
      A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

      Immunofluorescence:
      A previous lot of this antibody was used at 1:100-1:1,000 dilution.

      Immunoprecipitation:
      A previous lot of this antibody was used on Immunoprecipitation.

      ELISA:
      A previous lot of this antibody was used in ELISA.

      Optimal dilutions must be determined by the end user.
      Biological Information
      ImmunogenDenatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
      HostRabbit
      SpecificityTyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
      Species Reactivity
      • Human
      • Rat
      • Mouse
      • Feline
      • Ferret
      • Squid
      • Drosophila
      • Mollusk
      Species Reactivity NoteRat, mouse, ferret, feline, Aplysia (marine mollusc). It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryTyrosine hydroxylase is involved in the conversion of tyrosine to dopamine. As the rate-limiting enzyme in the synthesis of catecholamines, tyrosine hydroxylase has a key role in the physiology of adrenergic neurons.
      Gene Symbol
      • TH
      • TYH
      • EC 1.14.16.2
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P07101 # Plays an important role in the physiology of adrenergic neurons.
      COFACTOR: Fe(2+) ion.
      SIZE: 528 amino acids; 58524 Da
      TISSUE SPECIFICITY: Mainly expressed in the brain and adrenal glands.
      DISEASE: SwissProt: P07101 # Defects in TH are the cause of autosomal recessive Segawa syndrome [MIM:605407]; also known as DOPA-responsive dystonia. Typically, it begins in childhood or adolescence with progressive difficulty in walking and, in some cases, spasticity. Some cases present with parkinsonian symptoms in infancy and are referred to as autosomal recessive infantile parkinsonism.
      SIMILARITY: SwissProt: P07101 ## Belongs to the biopterin-dependent aromatic amino acid hydroxylase family.
      Molecular Weight62 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on PC12 lysates.

      Western Blot Analysis:
      1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      AB152 04053252287138

      Documentation

      Anti-Tyrosine Hydroxylase Antibody MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Anti-Tyrosine Hydroxylase Antibody Certificados de análise

      TítuloNúmero do lote
      Anti-Tyrosine Hydroxylase 3072361
      Anti-Tyrosine Hydroxylase - 2115918 2115918
      Anti-Tyrosine Hydroxylase - 2388829 2388829
      Anti-Tyrosine Hydroxylase - 2392225 2392225
      Anti-Tyrosine Hydroxylase - 2426518 2426518
      Anti-Tyrosine Hydroxylase - 2458991 2458991
      Anti-Tyrosine Hydroxylase - 1951919 1951919
      Anti-Tyrosine Hydroxylase - 1994651 1994651
      Anti-Tyrosine Hydroxylase - 2021194 2021194
      Anti-Tyrosine Hydroxylase - 2038257 2038257

      Referências

      Visão geral das referênciasAplicaçãoEspéciesPub Med ID
      The temporary and accumulated effects of transcranial direct current stimulation for the treatment of advanced Parkinson's disease monkeys.
      Li, H; Lei, X; Yan, T; Li, H; Huang, B; Li, L; Xu, L; Liu, L; Chen, N; Lü, L; Ma, Y; Xu, L; Li, J; Wang, Z; Zhang, B; Hu, X
      Scientific reports  5  12178  2015

      Mostrar Resumo
      26220760 26220760
      Expression of MYCN in Multipotent Sympathoadrenal Progenitors Induces Proliferation and Neural Differentiation, but Is Not Sufficient for Tumorigenesis.
      Mobley, BC; Kwon, M; Kraemer, BR; Hickman, FE; Qiao, J; Chung, DH; Carter, BD
      PloS one  10  e0133897  2015

      Mostrar Resumo
      26222553 26222553
      Rac1 plays an essential role in axon growth and guidance and in neuronal survival in the central and peripheral nervous systems.
      Hua, ZL; Emiliani, FE; Nathans, J
      Neural development  10  21  2015

      Mostrar Resumo
      26395878 26395878
      The Progressive BSSG Rat Model of Parkinson's: Recapitulating Multiple Key Features of the Human Disease.
      Van Kampen, JM; Baranowski, DC; Robertson, HA; Shaw, CA; Kay, DG
      PloS one  10  e0139694  2015

      Mostrar Resumo
      26439489 26439489
      VPS35 pathogenic mutations confer no dominant toxicity but partial loss of function in Drosophila and genetically interact with parkin.
      Malik, BR; Godena, VK; Whitworth, AJ
      Human molecular genetics  24  6106-17  2015

      Mostrar Resumo
      26251041 26251041
      Dopamine in the auditory brainstem and midbrain: co-localization with amino acid neurotransmitters and gene expression following cochlear trauma.
      Fyk-Kolodziej, BE; Shimano, T; Gafoor, D; Mirza, N; Griffith, RD; Gong, TW; Holt, AG
      Frontiers in neuroanatomy  9  88  2015

      Mostrar Resumo
      26257610 26257610
      A hepatic amino acid/mTOR/S6K-dependent signalling pathway modulates systemic lipid metabolism via neuronal signals.
      Uno, K; Yamada, T; Ishigaki, Y; Imai, J; Hasegawa, Y; Sawada, S; Kaneko, K; Ono, H; Asano, T; Oka, Y; Katagiri, H
      Nature communications  6  7940  2015

      Mostrar Resumo
      26268630 26268630
      Sexual dimorphism in the hypophysiotropic tyrosine hydroxylase-positive neurons in the preoptic area of the teleost, Clarias batrachus.
      Saha, S; Patil, S; Singh, U; Singh, O; Singru, PS
      Biology of sex differences  6  23  2015

      Mostrar Resumo
      26557978 26557978
      NMDA receptor GluN2A/GluN2B subunit ratio as synaptic trait of levodopa-induced dyskinesias: from experimental models to patients.
      Mellone, M; Stanic, J; Hernandez, LF; Iglesias, E; Zianni, E; Longhi, A; Prigent, A; Picconi, B; Calabresi, P; Hirsch, EC; Obeso, JA; Di Luca, M; Gardoni, F
      Frontiers in cellular neuroscience  9  245  2015

      Mostrar Resumo
      26217176 26217176
      Prenatal exposure to methylphenidate affects the dopamine system and the reactivity to natural reward in adulthood in rats.
      Lepelletier, FX; Tauber, C; Nicolas, C; Solinas, M; Castelnau, P; Belzung, C; Emond, P; Cortese, S; Faraone, SV; Chalon, S; Galineau, L
      The international journal of neuropsychopharmacology / official scientific journal of the Collegium Internationale Neuropsychopharmacologicum (CINP)  18  2015

      Mostrar Resumo
      25522388 25522388

      Brochura

      Título
      SNAP i.d. 2.0 System Brochure

      Informações técnicas

      Título
      Millipore Tools for Characterizing Induced Pluripotent Stem Cells