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MAB328 Anti-Oligodendrocytes Antibody, clone CE-1

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MAB328
100 µL  
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      Replacement Information

      Ofertas especiais

      Tabela com principais espec.

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Ch, H, M, Mk, R, FeIP, ICC, IHC, IH(P)MAscitesMonoclonal Antibody
      Description
      Catalogue NumberMAB328
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Oligodendrocytes Antibody, clone CE-1
      Alternate Names
      • MOSP
      References
      Product Information
      FormatAscites
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Oligodendrocytes Antibody, clone CE-1 is validated for use in IP, IC, IH, IH(P) for the detection of Oligodendrocytes.
      Key Applications
      • Immunoprecipitation
      • Immunocytochemistry
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Applications Not Recommended
      • Western Blotting
      Application NotesImmunocytochemistry

      Immunohistochemistry at 1:1,000-1:5000

      Not suggested for use in Western blot.

      Optimal working dilutions must be determined by end user.

      IMMUNOHISTOCHEMISTRY PROTOCOL FOR MAB328

      This antibody has been used successfully on 30 mm, free floating, 4% paraformaldehyde fixed rat brain tissue. All steps are performed under constant agitation. Suggested protocol follows.

      1) 3 x 10 minute washes in TBS (with or without 0.25% Triton).

      2) Incubate for 30 minutes in TBS with 3% serum (same as host from secondary antibody).

      3) Incubate primary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody) (with or without 0.25% Triton) for 2 hours at room temperature followed by 16 hours at 4°C.

      4) 3 x 10 minute washes in TBS.

      5) Incubate with secondary antibody diluted appropriately in TBS with 1% serum (same as host from secondary antibody).

      6) 3 x 10 minute washes in TBS.

      7) ABC Elite (1:200 Vector Labs) in TBS.

      8) 2 x 10 minute washes in TBS.

      9) 1 x 10 minute wash in phosphate buffer (no saline).

      10) DAB reaction with 0.06% NiCl added for intensification.

      11) 2 x 10 minute washes in PBS.

      12) 1 x 10 minute wash in phosphate buffer (no saline).
      Biological Information
      ImmunogenRat glial membranes and whole brain white matter.
      CloneCE-1
      HostMouse
      SpecificityIn neonatal rat glia MAB328 stains oligodendrocytes and does not react with type 1 astrocytes, type 2 astrocytes or fibroblasts. Does not stain cultures of rat Schwann cells. In tissue sections MAB328 labels oligodendrocytes and CNS myelin with no labeling of peripheral myelin. MAB328 detects MOSP which is expressed at day 4-5 of neonatal rat, which is about 1-2 after the appearance of GC or sulfatide. The initial expression of MOSP occurs at the stage in development when oligodendrocytes are elaborating processes and just beginning to form membrane sheets (Mu QQ & C Dyer, 1994).
      IsotypeIgM
      Species Reactivity
      • Chicken
      • Human
      • Mouse
      • Monkey
      • Rat
      • Feline
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      MAB328 04053252314865

      Documentation

      Anti-Oligodendrocytes Antibody, clone CE-1 MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Anti-Oligodendrocytes Antibody, clone CE-1 Certificados de análise

      TítuloNúmero do lote
      MOUSE ANTI-MYELIN/OLIGODENDROCYTE SPECIFIC PROTEIN 3050688
      MOUSE ANTI-MYELIN/OLIGODENDROCYTE SPECIFIC PROTEIN MONOCLONAL ANTIBODY - 2450209 2450209
      MOUSE ANTI-MYELIN/OLIGODENDROCYTE SPECIFIC PROTEIN - 3552766 3552766
      MOUSE ANTI-MYELIN/OLIGODENDROCYTE SPECIFIC PROTEIN -2854643 2854643
      MOUSE ANTI-MYELIN/OLIGODENDROCYTE SPECIFIC PROTEIN MONOCLONAL ANTIBODY 2931251
      MOUSE ANTI-MYELIN/OLIGODENDROCYTE SPECIFIC PROTEIN MONOCLONAL ANTIBODY - 2344682 2344682

      Referências

      Visão geral das referênciasAplicaçãoPub Med ID
      Strategies for repair of white matter: influence of osmolarity and microglia on proliferation and apoptosis of oligodendrocyte precursor cells in different basal culture media.
      Kleinsimlinghaus, K; Marx, R; Serdar, M; Bendix, I; Dietzel, ID
      Frontiers in cellular neuroscience  7  277  2013

      Mostrar Resumo
      24421756 24421756
      Frequent infection of cortical neurons by JC virus in patients with progressive multifocal leukoencephalopathy.
      Wüthrich, C; Koralnik, IJ
      Journal of neuropathology and experimental neurology  71  54-65  2012

      Mostrar Resumo
      22157619 22157619
      Altered expression pattern of testican-1 mRNA after brain injury.
      Ken Iseki,Seita Hagino,Yuxiang Zhang,Tetsuji Mori,Nobuko Sato,Sachihiko Yokoya,Yasukazu Hozumi,Kaoru Goto,Choichiro Tase
      Biomedical research (Tokyo, Japan)  32  2011

      Mostrar Resumo
      22199127 22199127
      Transplantation of predifferentiated adipose-derived stromal cells for the treatment of spinal cord injury.
      David Arboleda,Serhiy Forostyak,Pavla Jendelova,Dana Marekova,Takashi Amemori,Helena Pivonkova,Katarina Masinova,Eva Sykova
      Cellular and molecular neurobiology  31  2011

      Mostrar Resumo
      21630007 21630007
      Analysis of jmjd6 cellular localization and testing for its involvement in histone demethylation.
      Hahn P, Wegener I, Burrells A, Böse J, Wolf A, Erck C, Butler D, Schofield CJ, Böttger A, Lengeling A
      PLoS One  5  e13769.  2010

      Mostrar Resumo Texto completo do artigo
      21060799 21060799
      A common progenitor for retinal astrocytes and oligodendrocytes.
      Rompani, SB; Cepko, CL
      The Journal of neuroscience : the official journal of the Society for Neuroscience  30  4970-80  2010

      Mostrar Resumo
      20371817 20371817
      The ANKK1 gene associated with addictions is expressed in astroglial cells and upregulated by apomorphine.
      J Hoenicka, A Quinones-Lombrana, L Espana-Serrano, X Alvira-Botero, L Kremer, R Perez-Gonzalez, R Rodriguez-Jimenez, MA Jimenez-Arriero, G Ponce, T Palomo
      Biological psychiatry  67  3-11  2010

      Mostrar Resumo
      19853839 19853839
      Frequent infection of cerebellar granule cell neurons by polyomavirus JC in progressive multifocal leukoencephalopathy.
      Wüthrich, C; Cheng, YM; Joseph, JT; Kesari, S; Beckwith, C; Stopa, E; Bell, JE; Koralnik, IJ
      Journal of neuropathology and experimental neurology  68  15-25  2009

      Mostrar Resumo Texto completo do artigo
      19104450 19104450
      Morphological differentiation of tau-green fluorescent protein embryonic stem cells into neurons after co-culture with auditory brain stem slices.
      A Glavaski-Joksimovic, C Thonabulsombat, M Wendt, M Eriksson, H Ma, P Olivius, A Glavaski-Joksimovic, C Thonabulsombat, M Wendt, M Eriksson, H Ma, P Olivius, A Glavaski-Joksimovic, C Thonabulsombat, M Wendt, M Eriksson, H Ma, P Olivius
      Neuroscience  162  472-81  2009

      Mostrar Resumo
      19410633 19410633
      CD163, a marker of perivascular macrophages, is up-regulated by microglia in simian immunodeficiency virus encephalitis after haptoglobin-hemoglobin complex stimulation and is suggestive of breakdown of the blood-brain barrier.
      Juan T Borda,Xavier Alvarez,Mahesh Mohan,Atsuhiko Hasegawa,Andrea Bernardino,Sherrie Jean,Pyone Aye,Andrew A Lackner
      The American journal of pathology  172  2008

      Mostrar Resumo Texto completo do artigo
      18276779 18276779