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MABF985 Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5

Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 is an antibody against Eosinophil-Derived Neurotoxin for use in Western Blotting, ELISA, Multiplexing.

Ficha de dados de segurança do material (FDSM) ou ficha de segurança do material (FSM), certificado de análise (CA) e certificado de qualidade (CQ), dossiês e outros documentos disponíveis.
Research Category: Inflammation & Immunology Research Sub-Category: Inflammation & Autoimmune Mechanisms Gene Symbol: RNASE2, EDN, RAF3, RNS2 UniProt Number: P10153
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MABF985
100 μg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISA, MplexMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABF985
      DescriptionAnti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5
      Alternate Names
      • Non-secretory ribonuclease
      • Eosinophil-derived neurotoxin
      • Ribonuclease 2
      • Ribonuclease US
      • RNase 2
      • RNase UpI-2
      Background InformationNon-secretory ribonuclease (EC 3.1.27.5; UniProt P10153; also known as Eosinophil-derived neurotoxin, Ribonuclease 2, Ribonuclease US, RNase 2, RNase UpI-2) is encoded by the RNASE2 (also known as EDN, RAF3, RNS2) gene (Gene ID 6036) in human. The major basic protein (MBP), eosinophil cationic protein (ECP), eosinophil derived neurotoxin (EDN) and eosinophil peroxidase (EPO), are eosinophil effector molecules implicated in host defense, immunoregulatory responses, and in allergic and inflammatory reactions. These highly cationic proteins are stored preformed within the eosinophil secondary granules and released in intact membrane-bound granules during cell lysis or through a complex system of vesiculotubular structures during “piecemeal necrosis”. While eosinophil count and eosinophil surface expression of the activation marker CD69 are significantly correlated with serum concentrations of MBP, EDN and EPO, studies show that they are not correlated with ECP level. On the other hand, eosinophilic disease patients with normal eosinophil count show significantly increased concentrations of MBP, ECP, EDN, and EPO compared to normal donors. The measurement of MBP, ECP, EDN, and EPO levels thus provides a better predictor than eosinophil count alone in disease diagnosis. EDN is produced with a signal peptide sequence (a.a. 1-27), the removal of which yields the mature (a.a. 28-161) protein.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal IgG1 antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 is an antibody against Eosinophil-Derived Neurotoxin for use in Western Blotting, ELISA, Multiplexing.
      Key Applications
      • Western Blotting
      • ELISA
      • Multiplexing
      Application NotesWestern Blotting Analysis: 1.0 µg/mL from a representative lot detected eosinophil-derived neurotoxin in 50 µg of human liver and lung tissue lysates.
      ELISA Analysis: A representative lot was employed as the capture antibody for the detection of purified human EDN, as well as EDN in serum samples from individuals with eosinophilic disorders by sandwich ELISA (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22),
      Multiplexing Analysis: A representative lot was employed as the capture antibody in a bead-based multiplex assay for the detection of purified human EDN as well as EDN in serum samples from healthy donors and individuals with eosinophilic disorders. Prior reduction and alkylation of the serum samples (by DTT and iodoacetamide, respectively) resulted in higher EDN levels (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22),
      Biological Information
      ImmunogenPurified human EDN.
      Clone6D1.5/A5
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 6D1.5/A5 specifically reacted with EDN, but not other granule proteins by Western blotting analysis (Makiya, M.A., et al. (2014). J. Immunol. Methods. 411:11-22).
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • RNASE2
      • EDN
      • RAF3
      • RNS2
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~20 kDa observed. Target band appears larger than the calculated molecular weights of 18.35 kDa (pro-form) and 15.46 kDa (mature) due to glycosylation. Uncharacterized band(s) may appear in some lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human spleen tissue lysate.

      Western Blotting Analysis: 1.0 µg/mL of this antibody detected eosinophil-derived neurotoxin in 50 µg of human spleen tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      MABF985 04055977334425

      Documentation

      Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 MSDS

      Título

      Ficha de Segurança de Produtos (MSDS) 

      Anti-Eosinophil-Derived Neurotoxin Antibody, clone 6D1.5/A5 Certificados de análise

      TítuloNúmero do lote
      Anti-Eosinophil-Derived Neurotoxin, -2792900 2792900
      Anti-Eosinophil-Derived Neurotoxin, -2795635 2795635
      Anti-Eosinophil-Derived Neurotoxin, -2807719 2807719
      Anti-Eosinophil-Derived Neurotoxin, -Q2668632 Q2668632
      Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 - 3200634 3200634
      Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 - 3508321 3508321
      Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 - 4016279 4016279
      Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 Monoclonal Antibody 3091259
      Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5 Monoclonal Antibody 3035071
      Anti-Eosinophil-Derived Neurotoxin, clone 6D1.5/A5_2840391 2840391