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MABF414 Anti-CD4 Antibody (mouse), PE-Cy7, clone GK1.5

This rat monoclonal antibody Anti-CD4 (mouse), PE-Cy7, clone GK1.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD4.

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Research Category: Inflammation & Immunology Gene Symbol: Cd4 UniProt Number: P06332
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MABF414
100 µg  
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      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIP, IHC, FCRPE-Cy7Monoclonal Antibody
      Description
      Catalogue NumberMABF414
      DescriptionAnti-CD4 Antibody (mouse), PE-Cy7, clone GK1.5
      Alternate Names
      • T-cell surface glycoprotein CD4
      • T-cell differentiation antigen L3T4
      • T-cell surface antigen T4/Leu-3
      • CD4
      Background InformationThe GK1.5 antibody reacts with mouse CD4, a 55 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4 is typically expressed on thymocytes, certain mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The GK1.5 antibody is widely used as a phenotypic marker for CD4 expression. If used together, the GK1.5 antibody and an alternative antibody, Anti-Mouse CD4 clone RM4-5, will “compete” for binding, i.e. RM4-5 is able to block GK1.5 binding to cells. In contrast, the Anti-Mouse CD4 clone RM4-4 does not block binding of the GK1.5 antibody to cells (Arora S et al. 2006. Infect. Immun. 74: 4339-4348). The GK1.5 antibody is also reported to be cross-reactive with Syrian hamster CD4.
      References
      Product Information
      FormatPE-Cy7
      HS Code3002 15 90
      PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal antibody Anti-CD4 (mouse), PE-Cy7, clone GK1.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD4.
      Key Applications
      • Immunoprecipitation
      • Immunohistochemistry
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      CloneGK1.5
      Concentration0.2 mg/mL
      HostRat
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Cd4
      Purification MethodSize Exclusion Chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.25 µg of this antibody detected CD4 in one million C57Bl/6 splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Número de catálogo GTIN
      MABF414 04053252960055