Recommended Working Volumes
Single-well, 24-well and 96-well inserts are available to meet a range of application needs. The protocols printed in this section of the guide can be adjusted to accommodate a range of platforms. Please use the following table as a reference for scaling liquid additions in Millicell protocols.
|
|
Well Diameter (mm)
|
Membrane Surface Area (cm2)
|
Apical Volume (ml)
|
Basolateral Volume (ml) (24- or 96-well Receiver Tray)
|
Basolateral Volume (ml) (single-well Receiver Tray)
|
|---|
6-well standing inserts
|
30 |
4.2 |
1.5 |
2.0 |
n/a |
|---|
6-well hanging inserts
|
27 |
4.5 |
2.0 |
4.2* |
n/a |
|---|
| 12-well hanging inserts |
15 |
1.1 |
.4 |
2.0* |
n/a |
|---|
| 24-well standing inserts |
12 |
.6 |
.4 |
.6 |
n/a |
|---|
| 24-well hanging inserts |
9 |
.3 |
.2 |
1.3* |
n/a |
|---|
| Millicell-24 Cell Culture Insert Plate |
11 |
.7 |
.3 |
.6 |
28 |
|---|
Millicell-96 Cell Culture Insert Plate
|
5 |
.1 |
.075 |
.25 |
32 |
|---|
MultiScreen-MIC Filter Plate
|
7 |
.3 |
.075 |
.15 |
n/a |
|---|
* The basolateral volumes are measured in standard plastic culture plates with 18 mm well heights and are at equal head height with the apical well.