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71110 rLysozyme™ Solution

Stabilized recombinant lysozyme

rLysozyme™ Solution MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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71110
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Overview

Replacement Information

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71110-5
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      		 Plastic ampoule 6000 ku
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      Description
      OverviewrLysozyme™ Solution contains a highly purified and stabilized recombinant lysozyme that can be used for lysis of Gram-negative bacteria, such as E. coli. The enzyme catalyzes the hydrolysis of N-acetylmuramide linkages in bacterial cell walls. The specific activity of rLysozyme (1700 KU/mg) for E. coli lysis is 250 times greater than that of chicken egg white lysozyme. rLysozyme is optimally active at physiological pH. Very small amounts of rLysozyme (3–5 KU/g cell paste) enhance the efficiency of protein extraction with BugBuster®, BugBuster HT, and PopCulture® Reagents. In the absence of protein extraction reagents, direct lysis of E. coli can be achieved by treatment of 1.0 gram cell paste with 45–60 KU rLysozyme. The product is supplied as a ready-to-use solution at a concentration range of 27–33 KU/µl in 50% glycerol containing 50 mM Tris-HCl, 0.1 M NaCl, 0.1 mM EDTA, 1 mM DTT, 0.1% TRITON® X-100, pH 7.5. rLysozyme Solution is stable at –20°C.
      Catalogue Number71110
      Brand Family Novagen®
      References
      Product Information
      Unit of DefinitionOne unit of rLysozyme is defined as the amount of enzyme necessary to cause a decrease of 0.025 A₄₅₀ unit per min at 25°C in a 1-ml suspension (1 mg/ml) of Tuner™(DE3) cells in 0.5 x BugBuster diluted with 50 mM Tris-HCl, pH 7.5.
      Quality LevelMQ400
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71110-5 07790788053048

      Documentation

      rLysozyme™ Solution SDS

      Title

      Safety Data Sheet (SDS) 

      rLysozyme™ Solution Certificates of Analysis

      TitleLot Number
      71110

      Brochure

      Title
      Bulk Product Guide
      Protein Purification and Detection Tools

      Technical Info

      Title
      Benzonase®endonuclease for improved primary recovery in an E. coli-based process for Fab production
      TB334 rLysozyme Solution

      Citations

      Title
    • Rachel Balder, et al. (2007) Moraxella catarrhalis strain O35E expresses two filamentous hemagglutinin-like proteins that mediate adherence to human epithelial cells. Infection and Immunity 75, 2765-2775.
    • Benedict M. Long, et al. (2007) Analysis of carboxysomes from Synechococcus PCC7942 reveals multiple rubisco complexes with carboxysomal proteins CcmM and CcaA. Journal of Biological Chemistry 282, 29323-29335.
    • Sehyung Cho, et al. (2005) Glucocorticoid receptor ligand binding domain is sufficient for the modulation of glucocorticoid induction properties by homologous receptors, coactivator transcription intermediary factor 2, and ubc9. Molecular Endocrinology 19, 290-311.
    • S. Moy, et al. (2004) Genome-scale expresion of proteins from Bacillus subtilis. Journal of Structural and Functional Genomics 5, 103-109.
    • Mark A. Rizzo, et al. (2004) An improved cyan fluorescent protein variant useful for FRET. Nature Biotechnology 22, 445-449.
    • B. Husen, et al. (2003) Characterization of 17β-hydroxysteroid dehygronase type 7 in reproductive tissues of the marmoset monkey. Biology of Reproduction 68, 2092-2099.
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      User Protocols

      Title
      TB334 rLysozyme™ Solution