Millipore Sigma Vibrant Logo

484235 p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem

p-Nitroblue Tetrazolium Chloride, CAS 298-83-9, is a NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: NBT, Nitro BT

Primary Target: NOS
View Products on Sigmaaldrich.com
484235
View Pricing & Availability

Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
298-83-9C₄₀H₃₀Cl₂N₁₀O₆

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
484235-1GM
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Glass bottle 1 gm
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      Description
      OverviewNADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).
      Catalogue Number484235
      Brand Family Calbiochem®
      SynonymsNBT, Nitro BT
      References
      ReferencesPistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
      De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis. 16, 29.
      Libon, C., et al. 1993. J. Leukoc. Biol. 53, 93.
      Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
      Nichols, K., et al. 1992. Neuroscience 51, 791.
      Nichols, K., et al. 1992. Neuroscience 51, 791.
      Product Information
      CAS number298-83-9
      FormYellow crystalline solid
      Hill FormulaC₄₀H₃₀Cl₂N₁₀O₆
      Chemical formulaC₄₀H₃₀Cl₂N₁₀O₆
      Hygroscopic Hygroscopic
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      Applicationp-Nitroblue Tetrazolium Chloride, CAS 298-83-9, is a NADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions.
      Biological Information
      Primary TargetNOS
      Primary Target IC<sub>50</sub>3-4 µM
      Purity≥98% by TLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSXF8045000
      Safety Information
      R PhraseR: 20/21/22-68

      Harmful by inhalation, in contact with skin and if swallowed.
      Possible risks of irreversible effects.
      S PhraseS: 22-26-36/37

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing and gloves.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      484235-1GM 07790788054755

      Documentation

      p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      p-Nitroblue Tetrazolium Chloride - CAS 298-83-9 - Calbiochem Certificates of Analysis

      TitleLot Number
      484235

      References

      Reference overview
      Pistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
      De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis. 16, 29.
      Libon, C., et al. 1993. J. Leukoc. Biol. 53, 93.
      Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
      Nichols, K., et al. 1992. Neuroscience 51, 791.
      Nichols, K., et al. 1992. Neuroscience 51, 791.

      Brochure

      Title
      Pathways and Biomarkers of Oxidative Stress
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision13-August-2008 RFH
      SynonymsNBT, Nitro BT
      DescriptionNADPH-diaphorase substrate that competitively inhibits nitric oxide synthase (IC50 = 3-4 µM). A well-known scavenger of superoxide anions. Useful as a substrate for alkaline phosphatase, often in conjunction with BCIP (Cat. No. 203788).
      FormYellow crystalline solid
      CAS number298-83-9
      RTECSXF8045000
      Chemical formulaC₄₀H₃₀Cl₂N₁₀O₆
      Structure formulaStructure formula
      Purity≥98% by TLC
      SolubilityMeOH or H₂O
      Storage Protect from light
      +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long-term storage or refrigerate (4°C) for short-term storage.
      Toxicity Harmful
      ReferencesPistelli, A., et al. 1994. Biochem. Pharmacol. 47, 1737.
      De la Fuente, M., et al. 1993. Comp. Immunol. Microbiol. Infect. Dis. 16, 29.
      Libon, C., et al. 1993. J. Leukoc. Biol. 53, 93.
      Mittal, C.K. 1993. Biochem. Biophys. Res. Commun. 193, 126.
      Nichols, K., et al. 1992. Neuroscience 51, 791.
      Nichols, K., et al. 1992. Neuroscience 51, 791.