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598226 Thiostrepton - CAS 1393-48-2 - Calbiochem

Thiostrepton, CAS 1393-48-2, is an antibiotic that inhibits protein synthesis by preventing binding of GTP to 50S ribosomal subunit.

Thiostrepton - CAS 1393-48-2 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: FOXM1 Inhibitor I

Primary Target: EF-G
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598226
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
1393-48-2C₇₂H₈₅N₁₉O₁₈S₅

Pricing & Availability

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      Description
      OverviewA thiazole-containing peptide antibiotic that inhibits protein synthesis by preventing binding of GTP to 50S ribosomal subunit. Inhibits the function of elongation factor G (EF-G) and the dissociation of EF-G from the ribosome. The thiostrepton-resistant gene is also commonly used as a selective marker for recombinant DNA/plasmid technologies.
      Catalogue Number598226
      Brand Family Calbiochem®
      SynonymsFOXM1 Inhibitor I
      References
      ReferencesRodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA 96, 9586.
      Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res. 22, 357.
      Saarma, U. and Remme, J. 1992. Nucleic Acids Res. 20, 3147.
      Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol. 58, 895.
      Blanco, G., et al. 1992. Gene 112, 59.
      Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res. 19, 1657.
      Ryan, P.C., et al. 1991. J. Mol. Biol. 221, 1257.
      Kutay, U.R., et al. 1990. Biochim. Biophys. Acta 1050, 193.
      Smokvina, T., et al. 1990. Gene 94, 53.
      Garrett, R. 1983. Trends Biochem. Sci. 8, 189.
      Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun. 41, 1406.
      Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.
      Product Information
      CAS number1393-48-2
      FormOff-white powder
      Hill FormulaC₇₂H₈₅N₁₉O₁₈S₅
      Chemical formulaC₇₂H₈₅N₁₉O₁₈S₅
      Quality LevelMQ100
      Applications
      Biological Information
      Primary TargetEF-G
      Purity≥98% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      RTECSXN6300100
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Protect from Moisture Protect from moisture
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      598226-1GM 07790788051914

      Documentation

      Thiostrepton - CAS 1393-48-2 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Thiostrepton - CAS 1393-48-2 - Calbiochem Certificates of Analysis

      TitleLot Number
      598226

      References

      Reference overview
      Rodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA 96, 9586.
      Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res. 22, 357.
      Saarma, U. and Remme, J. 1992. Nucleic Acids Res. 20, 3147.
      Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol. 58, 895.
      Blanco, G., et al. 1992. Gene 112, 59.
      Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res. 19, 1657.
      Ryan, P.C., et al. 1991. J. Mol. Biol. 221, 1257.
      Kutay, U.R., et al. 1990. Biochim. Biophys. Acta 1050, 193.
      Smokvina, T., et al. 1990. Gene 94, 53.
      Garrett, R. 1983. Trends Biochem. Sci. 8, 189.
      Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun. 41, 1406.
      Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.

      Brochure

      Title
      Antibiotics Profiler

      Citations

      Title
    • Sarae L. Bausch, Ekaterina Poliakova and David E. Draper. (2005) Interactions of the N-terminal domain of ribosomal protein L11 with thiostrepton and rRNA. Journal of Biological Chemistry 280, 29956-29963.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-August-2014 JSW
      SynonymsFOXM1 Inhibitor I
      DescriptionA thiazole-containing polypeptide antibiotic isolated from Streptomyces azureus. Inhibits bacterial protein synthesis and ribosomal GTPase activity by binding noncovalently, but virtually irreversibly, to the 23S rRNA in the GTPase center of the 50S subunit. Thiostrepton binding directly prevents binding to the ribosome of elongation factor G (EF-G), which also binds specifically to the 23S rRNA. Thiostrepton has proved useful in elucidating the molecular mechanisms of translation, particularly of ribosomal translocation. It is also used for selection, in bacteria of Streptomyces sp., of vectors containing the tsr gene (S. azureus), which encode a 23S rRNA methylase whose action prevents thiostrepton from binding to ribosomes.
      FormOff-white powder
      CAS number1393-48-2
      RTECSXN6300100
      Chemical formulaC₇₂H₈₅N₁₉O₁₈S₅
      Purity≥98% by HPLC
      SolubilityDMSO (10 mg/ml) or Chloroform (10 mg/ml)
      Storage Protect from moisture
      Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      Merck USA index14, 9364
      ReferencesRodnina, M.V., et al. 1999. Proc. Natl. Acad. Sci USA 96, 9586.
      Rosendahl, G. and Douthwaite, S. 1994. Nucleic Acids Res. 22, 357.
      Saarma, U. and Remme, J. 1992. Nucleic Acids Res. 20, 3147.
      Malina, H. and Robert-Gero, M. 1992. Appl. Environ. Microbiol. 58, 895.
      Blanco, G., et al. 1992. Gene 112, 59.
      Miller, S.P. and Bodley, J.W. 1991. Nucleic Acids Res. 19, 1657.
      Ryan, P.C., et al. 1991. J. Mol. Biol. 221, 1257.
      Kutay, U.R., et al. 1990. Biochim. Biophys. Acta 1050, 193.
      Smokvina, T., et al. 1990. Gene 94, 53.
      Garrett, R. 1983. Trends Biochem. Sci. 8, 189.
      Bodley, J.W., et al. 1970. Biochem. Biophys. Res. Commun. 41, 1406.
      Thompson, J. and Cundliffe, E. 1970. Biochimie 73, 1131.
      Citation
    • Sarae L. Bausch, Ekaterina Poliakova and David E. Draper. (2005) Interactions of the N-terminal domain of ribosomal protein L11 with thiostrepton and rRNA. Journal of Biological Chemistry 280, 29956-29963.
      		•