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FCMAB100P Milli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE

This Milli-Mark Phospho-Erk1/2 antibody is validated for use in Flow & WB for the detection of the Milli-Mark Phospho-Erk1/2 protein.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: MAP Kinases Gene Symbol: Erk1, Erk2, p44-MAPK, p42-MAPK, MAPK2, PRKM2, p41mapk, PRKM1, p44, ERK2, P42MAPK, ERK, p42, MAPK1, ERT1, ERT2, Mitogen-activated protein kinase, MAP Kinase 2, MAP Kinase 1, MAPK UniProt Number: P28482
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FCMAB100P
100 tests  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, M, RFC, WBRbPEMonoclonal Antibody
      Description
      Catalogue NumberFCMAB100P
      Trade Name
      • Milli-Mark
      DescriptionMilli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE
      Alternate Names
      • Erk1
        Erk2
        p44-MAPK
        p42-MAPK
        MAPK2
        PRKM2
        p41mapk
        PRKM1
        p44
        ERK2
        P42MAPK
        ERK
        p42
        MAPK1
        ERT1
        ERT2
        Mitogen-activated protein kinase
        MAP Kinase 2
        MAP Kianse 1
        MAPK
      Background InformationErk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).
      References
      Product Information
      FormatPE
      HS Code3002 15 90
      Control
      • Jurkat Cells
      PresentationPurified Rabbit monoclonal supernatant conjugated to phycoerythrin in buffer containing PBS with 0.05% sodium azide
      Quality LevelMQ100
      Applications
      ApplicationThis Milli-Mark Phospho-Erk1/2 antibody is validated for use in Flow & WB for the detection of the Milli-Mark Phospho-Erk1/2 protein.
      Key Applications
      • Flow Cytometry
      • Western Blotting
      Biological Information
      ImmunogenPhosphorylated recombinant peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated
      EpitopePhosphorylated Thr185/Phosphorylated Tyr187
      CloneAW39R
      HostRabbit
      SpecificityAntibody recognizes Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      • Rat
      Species Reactivity NoteTested and passed on Human. based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. The activation of this kinase requires its phosphorylation by upstream kinases. Upon activation, this kinase translocates to the nucleus of the stimulated cells, where it phosphorylates nuclear targets. Two alternatively spliced transcript variants encoding the same protein, but differing in the UTRs, have been reported for this gene.
      Gene Symbol
      • Erk1
      • Erk2
      • p44-MAPK
      • p42-MAPK
      • MAPK2
      • PRKM2
      • p41mapk
      • PRKM1
      • p44
      • ERK2
      • P42MAPK
      • ERK
      • p42
      • MAPK1
      • ERT1
      • ERT2
      • Mitogen-activated protein kinase
      • MAP Kinase 2
      • MAP Kinase 1
      • MAPK
      Modifications
      • Phosphorylation
      Purification MethodProtein A Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P28482 # Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
      COFACTOR: Magnesium (By similarity).
      SIZE: 360 amino acids; 41390 Da
      SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef through its SH3 domain. This interaction inhibits its tyrosine- kinase activity. Interacts with HSF4.
      DOMAIN: SwissProt: P28482 The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
      PTM: Dually phosphorylated on Thr-185 and Tyr-187, which activates the enzyme.
      SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily. & Contains 1 protein kinase domain.
      Molecular Weight 42kDa and 44kDa Observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Flow Cytometry with Jurkat cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 6 months at 2-8°C from date of receipt. Protect from light.
      Packaging Information
      Material Size100 tests
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      FCMAB100P 04053252734014

      Documentation

      Milli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE SDS

      Title

      Safety Data Sheet (SDS) 

      Milli-Mark Anti-Phospho-Erk1/2 Antibody (Thr202/Tyr204, Thr185/Tyr187)-PE Certificates of Analysis

      TitleLot Number
      Milli-Mark Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - 1990431 1990431
      Milli-Mark Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - 2049265 2049265
      Milli-Mark Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - 2154104 2154104
      Milli-Mark Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - 2202481 2202481
      Milli-Mark Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - JH1741005 JH1741005
      Milli-Mark Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - NG1798883 NG1798883
      Milli-Mark Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - NG1925928 NG1925928
      Milli-Mark™ Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - 3727141 3727141
      Milli-Mark™ Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - 4058481 4058481
      Milli-Mark™ Phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187) -PE - 4104658 4104658
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      Categories

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