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ASK10 MMP/TIMP Family Antibody Sampler Kit I

This MMP/TIMP Family Antibody Sampler Kit I is validated for use in Frozen Sections, Immunoblotting, IP, Paraffin Sections for the detection of MMP/TIMP Family Antibody Sampler Kit I.

MMP/TIMP Family Antibody Sampler Kit I MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
ASK10
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      Description
      OverviewContains 20 µg of each antibody. Includes Anti-MMP-1 (Cat. No. IM35), Anti-MMP-2 (Cat. No. IM33), Anti-MMP-3 (Cat. No. IM36), Anti-MMP-9 (Cat. Nos. IM09 and IM37), Anti-TIMP-1 (Cat. No. IM32), and Anti-TIMP-2 (Cat. No. IM11). Please see individual product descriptions for more information.
      Catalogue NumberASK10
      Brand Family Calbiochem®
      Application Data
      References
      ReferencesHöyhtyä, M., et al. 1994. Int. J. Cancer. 56, 500.
      Cottam, D. W. and Rees, R. C. 1993. Intl J. Oncol. 2, 861.
      Fujimoto, N., et al. 1993. Clinica Chimica Acta. 221, 91.
      Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
      Stetler-Stevenson, W. G., et al. 1993. FASEB J. 7, 1434.
      Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1.
      Zucker, S., et al. 1993. Cancer Res. 53, 140.
      Obata, K., et al. 1992. Clinica Chimica Acta. 211, 59.
      Woessner, J. F. 1991. FASEB J. 5, 2145.
      Liotta, L. A. and Stetler-Stevenson, W. G. 1990. in Seminars in Cancer Biology, ed. M. M. Gottesman. Vol.1(2); 99-102.
      Kodama, S., et al. 1987. Collagen Rel. Res. 7, 341.
      Product Information
      DeclarationManufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
      FormLiquid
      Formulation20 µg of each antibody in 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.4.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesMMP-9 (Ab-2) Frozen and Paraffin Sections Aikawa, M, et al. 1998. Circ. Res. 83, 1015. MMP-2 (Ab-3) Paraffin Sections: Tsuchiya, Y., et al. 1994. Int. J. Cancer. 56, 46. MMP-1 (Ab-1), MMP-2 (Ab-3), and MMP-3 (Ab-1) Frozen Sections Nakagawa, T., et al. 1994. J. Neurosurg. 81, 69. MMP-1 (Ab-1) Paraffin Sections: Zhang, J., et al. 1993. Clinica Chimica Acta. 219, 1. MMP-2 (Ab-3) Paraffin Sections Nomura H., et al. 1996. Int. J. Cancer 69, 9. Obata, K., et al. 1992. Clinica Chimica Acta. 211, 59. (called clone B) MMP-9 (Ab-1) Enzyme Inhibition: Ramos-DeSimone, N., et al. 1993. Hybridoma 12, 349. MMP-9 (Ab-1) and MMP-9 (Ab-2) Immunoprecipitation Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci. 732, 469. TIMP-1 (Ab-1) Imunohistochemistry Okada, Y., et al. 1990. Virchows Arch. B Cell Pathol. 59, 305. TIMP-2 (Ab-1) Immunoprecipitation and Frozen Sections Höyhtyä, M., et al. 1994. Int. J. Cancer. 56, 500.
      Application NotesFrozen Sections (see characteristics below)
      Immunoblotting (see characteristics below)
      Immunoprecipitation (see characteristics below)
      Paraffin Sections (see characteristics below)
      Application CommentsFor an MMP-1 or MMP-3 positive control, incubate HT-1080 cells for 2 hours at 37°C in serum-free media containing 100 nM PMA. Collect and centrifuge medium; concentrate as necessary. Store in aliquots at -20°C. When staining paraffin sections for MMP-1, pretreatment with heat, saponin, or trypsin can be used, but a pressure cooker generally gives the best results. Anti-MMP-9 (Ab-1) recognizes both the latent and active forms of MMP-9 under non-reducing conditions but only the latent form under reducing conditions. Anti-MMP-9 (Ab-1) will inhibit MMP-9 enzymatic activity. Anti-MMP-9 (Ab-2) recognizes only the latent form of MMP-9 (non-reducing or reducing conditions). Anti-TIMP-1 (Ab-1) will react with TIMP-1 and MMP/TIMP-1 complexes. Antibodies should be titrated for optimal results in individual systems.
      Biological Information
      Concentration Label Please refer to vial label for lot-specific concentration
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      ASK10-1EA 04055977212761