Millipore Sigma Vibrant Logo

HSCRMAG-32K MILLIPLEX MAP Human Soluble Cytokine Receptor Panel- Immunology Multiplex Assay

Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Inflammation & Immunology
View Products on Sigmaaldrich.com
HSCRMAG-32K
Design And Price Your Kit

Overview

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
sCD30 sEGFR sgp130 sIL-1RI sIL-1RII sIL-2Rα sIL-4R sIL-6R sRAGE sTNFRI sTNFRII sVEGF-R1 sVEGF-R2 sVEGF-R3 HMplexLuminex xMAP
Description
Catalogue NumberHSCRMAG-32K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Human Soluble Cytokine Receptor Panel- Immunology Multiplex Assay
Background InformationCytokine receptors constitute an integral part of cytokine biology. Like cytokines, cytokine receptors are involved in normal physiological and pathological processes of almost all disease states. Soluble cytokine receptors naturally arise from genes encoding membrane- bound receptors or are direct derivatives of the receptors themselves. The discovery that soluble cytokine receptors are involved in regulating excessive inflammatory responses and modulating immune events has stimulated significant research interest in their potential role as immunotherapeutic agents. Many of these soluble cytokine receptors have the ability to inhibit the binding and biological activity of their cytokine ligands, making them very specific cytokine antagonists.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <15%
  • Intra-assay: <10%
Panel TypeMAGNETIC Cytokines/Chemokines
Quality LevelMQ200
Applications
ApplicationSimultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in human serum, plasma and cell culture samples.
Key Applications
  • Multiplexing
Application Notes• This is an overnight incubation assay with a 1-hour incubation with detection antibodies requiring 25 µL of a 1:5 dilution for plasma or serum, or 25 μL cell culture supernatant per well.
• This kit may be used for the analysis of all or any combination of the Human Soluble Cytokine Receptor panel analytes in serum, plasma or tissue/cell lysate and culture supernatant.
Biological Information
Species Reactivity
  • Human
Analytes Available
  • sCD30
  • sEGFR
  • sgp130
  • sIL-1RI
  • sIL-1RII
  • sIL-2Rα
  • sIL-4R
  • sIL-6R
  • sRAGE
  • sTNFRI
  • sTNFRII
  • sVEGF-R1
  • sVEGF-R2
  • sVEGF-R3
Cross ReactivityThere is no detectable cross-reactivity within the panel.
Accuracy
  • 91% – 104%
Physicochemical Information
Sensitivity
  • 4 - 208 pg /mL (Overnight Incubation minDC)
  • Please see kit protocol for individual assay sensitivities.
Linearity of DilutionPlease contact technical service for information
Standard Curve Range
  • sCD30, sgp130, sIL-1RI, sIL-2Rα: 24.4 – 100,000 pg/mL
  • sEGFR, sIL-1RII, sVEGF-R1, sVEGF-R2, sVEGF-R3:122.1 – 500,000 pg/mL
  • sIL-4R, sIL-6R, sRAGE, sTNFRI, sTNFRII: 12.2 - 50,000 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Global Trade Item Number
Catalogue Number GTIN
HSCRMAG-32K 04053252987625