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71086 KOD Hot Start DNA Polymerase

High fidelity DNA polymerase designed for accurate PCR amplification of long strand and GC- rich DNA templates for cloning and cDNA amplification applications.

KOD Hot Start DNA Polymerase MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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71086
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      Description
      OverviewPCR involves replication of a DNA template by a thermostable DNA polymerase. The processivity, specificity, and fidelity of the polymerase enzyme used can influence the efficiency, reproducibility, and yield of the PCR reaction. High-fidelity PCR, utilizes a DNA polymerase with a low error rate and results in a high degree of accuracy in the replication of the DNA of interest. Fidelity is critical when accurate sequence amplification of the gene target is needed, for example, when direct sequencing or cloning for downstream protein expression. Unwarranted mutation could severely impact your studies. Our analysis has shown that KOD enzymes are an easy choice for fast, accurate and high-yielding PCR. EMD Millipore's molecular biologists work to develop and formulate polymerases offering the highest specificity, fidelity and yield during PCR amplification. In addition, optimized buffer compositions, convenient master mixes and cycling parameters provide additional ease of use and data reproducibility. KOD Hot Start DNA Polymerase* is a premixed complex of KOD DNA Polymerase and two monoclonal antibodies that inhibit the DNA polymerase and 3'→5' exonuclease activities at ambient temperatures (Mizuguchi 1999). KOD Hot Start amplifies genomic DNA templates up to 21 kb including GC-rich genes for PCR applications. KOD Hot Start combines the high fidelity, fast extension speed, and outstanding processivity of KOD with the high specificity of an antibody-mediated hot start. Non-specific amplification is reduced because mispriming events that can occur during setup and initial temperature increase are avoided. In addition, primer degradation during setup at room temperature due to exonuclease activity is effectively inhibited. KOD Hot Start DNA Polymerase generates blunt-ended PCR products suitable for cloning with the Novagen Perfectly Blunt® and LIC Vector Kits.

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      Source Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli
      Concentration 1.0 U/µl
      Nicking activity None detected
      Amplification effiency Functional PCR; inhibition of activity at 21°C verified
      Storage –20°C

      *Manufactured by Toyobo and distributed by EMD. Not available in Japan.

      Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.

      Catalogue Number71086 Brand Family Novagen®
      Features and benefits
      • Highest accuracy, yield, and processivity of commercially available proofreading DNA polymerases
      • Amplifies genomic DNA templates up to 12 kbp
      • Amplifies plasmid and lambda DNA templates up to 21 kbp
      • Successfully amplifies GC-rich sequences
      • Eliminates mispriming and primer-dimer formation
      • Convenient room-temperature setup compatible with automation
      • Optimal KOD Hot Start Buffer for PCR performance over a wide range of targets
      References
      ReferencesMizuguchi, H., et al. 1999. J. Biochem. (Tokyo) 126, 762. Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol. 289, 835.
      Product Information
      Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl₂, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [<Sup>3</Sup>H]dTTP), and 150 µg/ml activated calf thymus DNA.
      Components
      DeclarationManufactured by Toyobo and distributed by EMD. Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
      Quality LevelMQ200
      Applications
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      71086-3 07790788053017

      Documentation

      KOD Hot Start DNA Polymerase Certificates of Analysis

      TitleLot Number
      71086

      References

      Reference overview
      Mizuguchi, H., et al. 1999. J. Biochem. (Tokyo) 126, 762. Kitabayashi, M., et al. 2002. Biosci. Biotechnol. Biochem. 66 (10), 2194. Fujii, S., et al. 1999. J. Mol. Biol. 289, 835.

      Brochure

      Title
      High fidelity gene amplification
      PCR Protocols and Guides - Simplify your gene discovery
      The Complete Molecular Biology Toolkit - Expert workflow solutions from DNA cloning to protein expression

      Citations

      Title
    • Elena Curti, Stephen J. Smerdon and Elaine O. Davis. (2007) Characterization of the helicase activity and substrate specificity of Mycobacterium tuberculosis UvrD. Journal of Bacteriology 189, 1542-1555.
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    • Changkeun Lee, et al. (2007) Yeast mitochondrial initiator tRNA is methylated at G37 by the TRM5-encoded tRNA (Guanine-N1-)-methyltransferase. Journal of Biological Chemistry 282, 27744-27753.
    • Bin Li, et al. (2007) FOXP3 interactions with histone acetyltransferase and class II histone deacetylases are required for repression. Procedings of the National Academy of Science 104, 4571-4576.
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    • Anna K. Overby, Ralf F. Pettersson and Etienne P. A. Neve. (2007) The Glycoprotein Cytoplasmic Tail of Uukuniemi Virus (Bunyaviridae) Interacts with Ribonucleoproteins and Is Critical for Genome Packaging. Journal of Virology 81, 3198-3205.
    • Ken-ichiro Taoka, et al. (2007) Reciprocal phosphorylation and glycosylation recognition motifs control NCAPP1 interaction with pumpkin phloem proteins and their cell-to-cell movement. Plant Cell 19, 1866-1884.
    • David M. Cauvi, Gabrielle Cauvi and K. Michael Pollard. (2006) Constitutive expression of murine decay-accelerating factor 1 is controlled by the transcription factor Sp1. Journal of Immunology 177, 3837-3847.
    • Lata T. Gooljarsingh, et al. (2006) A biochemical rationale for the anticancer effects of Hsp90 inhibitors: Slow, tight binding inhibition by geldanamycin and its analogues. Proceedings of the National Academy of Sciences (USA) 103, 7625-7630.
    • Lijoy K. Mathew, Eric A. Andreasen and Robert L. Tanguay. (2006) Aryl hydrocarbon receptor activation inhibits regenerative growth. Molecular Pharmacology 69, 257-265.
    • Sambit K. Nanda and Michael D. Baron. (2006) Rinderpest virus blocks type I and type II interferon action: role of structural and nonstructural proteins. Journal of Virology 80, 7555-7568.
    • Motoko Unoki, et al. (2006) Novel splice variants of ING4 and their possible roles in regulation of cell growth and motility. Journal of Biological Chemistry 281, 34677-34686.
    • Brock F. Binkowski, et al. (2005) Correcting errors in synthetic DNA through consensus shuffling. Nucleic Acids Research 33, e66.
    • Sean Crosson, et al. (2005) Conserved modular design of an oxygen sensory/signaling network with species-specific output. Proceedings of the National Academy of Sciences (USA) 102, 8018-8023.
    • Kathryn H. Loomis, et al. (2005) InsectDirectTM System: rapid, high-level protein expression and purication from insect cells. Journal of Structural and Functional Genomics 6, 189-194.
    • Oliver Schilling, et al. (2005) Exosite modules guide substrate recognition in the ZiPD/ElaC protein family. Journal of Biological Chemistry 280, 17857-17862.
    • Marko Tammenkoski, et al. (2005) An unusual, his-dependent family I pyrophosphatase from Mycobacterium tuberculosis. Journal of Biological Chemistry 280, 41819-41826.
    • Mark E. Williams, et al. (2005) Ric-3 promotes functional expression of the nicotinic acetylcholine receptor 7 subunit in mammalian cells. Journal of Biological Chemistry 280, 1257-1263.
    • Jean-Francois Rual, et al. (2004) Human ORFeome version 1.1: a platform for reverse proteomics. Genome Research 14, 2128-2135.
    • Xinxin Gao, et al. (2003) Thermodynamically balanced inside-out (TBIO) PCR-based gene synthesis: a novel method of primer design for high-fidelity assembly of longer gene sequences. Nucleic Acids Research 31, e143-.
    • Tomoko Miyazato, et al. (2003) Molecular analysis of VcfQ protein involved in Vibrio cholerae type IV pilus biogenesis. Medical Microbiology 52, 283-288.
    • Takaaki Sato, et al. (2003) Targeted gene disruption by homologous recombination in the hyperthermophilic Archaeon Thermococcus kodakaraensis KOD1. Journal of Bacteriology 185, 210-220.
    • Yoshihiko Hirohashi, et al. (2002) An HLA-A24-restricted cytotoxic T lymphocyte epitope of a tumor-associated protein, survivin. Clinical Cancer Research 8, 1731-1739.
    • Takeshi Okamoto, et al. (2002) A change in PBP1 is involved in amoxicillin resistance of clinical isolates of Helicobacter pylori. Journal of Antimicrobial Chemotherapy 50, 849-856.
    • Mitsuaki Tabuchi, et al. (2002) Alternative splicing regulates the subcellular localization of divalent metal transporter 1 isoforms. Molecular Biology of the Cell 13, 4371-4387.
    • Harumi Terasaki, et al. (2002) Frizzled-10, up-regulated in primary colorectal cancer, is a positive regulator of the WNT-β-catenin-TCF signaling pathway. International Journal of Molecular Medicine 9, 107-112.
    • Masaki Watanabe, et al. (2002) Molecular characterization of a novel β1,3-galactosyltransferase for capsular polysaccharide synthesis by Streptococcus agalactiae type Ib. 131, 183-191.
    • Tokinori Iwamotob, et al. (2001) Establishment of an infectious RNA transcription system for striped jack nervous necrosis virus, the type species of the betanodaviruses. Journal of General Virology 82, 2653-2662.
    • Naoki Matsumoto, et al. (2001) H-2 allele specificity of the NK cell C-type lectin-like MHC class I receptor Ly49A visualized by soluble Ly49A tetramer. International Immunology 13, 615-623.
    • Naoki Matsumotoa, et al. (2001) The functional binding site for the C-type lectin-like natural killer cell receptor Ly49A spans three domains of its major histocompatibility complex class I ligand. Journal of Experimental Medicine 193, 147-158.
    • Norihiko Sagara and Masaru Katoh. (2000) Mitomycin C resistance induced by TCF-3 overexpression in gastric cancer cell line MKN28 is associated with DT-diaphorase down-regulation. Cancer Research 60, 5959-5962.
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      User Protocols

      Title
      TB341 KOD Hot Start DNA Polymerase

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