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189490 Autophagy Inhibitor, 3-MA - CAS 5142-23-4 - Calbiochem

Autophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker that protects cerebellar granule cells from apoptosis following serum/potassium deprivation.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: 3-Methyladenine

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189490
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Overview

Replacement Information

Key Spec Table

CAS #Empirical Formula
5142-23-4C₆H₇N₅

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189490-100MG
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      Description
      OverviewA widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)
      Catalogue Number189490
      Brand Family Calbiochem®
      Synonyms3-Methyladenine
      References
      ReferencesCanu, N., et al. 2005. J. Neurochem. 92, 1228.
      Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun. 316, 845.
      Beugnet, A., et al. 2003. Biochem. J. 372, 555.
      Eskelinen, E.L., et al. 2002. Traffic 3, 878.
      Petiot, A., et al. 2000. J. Biol. Chem. 275, 992.
      Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA 79, 1889.
      Product Information
      CAS number5142-23-4
      FormWhite to faint yellow powder
      Hill FormulaC₆H₇N₅
      Chemical formulaC₆H₇N₅
      Structure formula ImageStructure formula Image
      Quality LevelMQ100
      Applications
      ApplicationAutophagy Inhibitor, 3-MA, CAS 5142-23-4, is a cell-permeable autophagic sequestration blocker that protects cerebellar granule cells from apoptosis following serum/potassium deprivation.
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      189490-100MG 04055977221831

      Documentation

      Autophagy Inhibitor, 3-MA - CAS 5142-23-4 - Calbiochem SDS

      Title

      Safety Data Sheet (SDS) 

      Autophagy Inhibitor, 3-MA - CAS 5142-23-4 - Calbiochem Certificates of Analysis

      TitleLot Number
      189490

      References

      Reference overview
      Canu, N., et al. 2005. J. Neurochem. 92, 1228.
      Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun. 316, 845.
      Beugnet, A., et al. 2003. Biochem. J. 372, 555.
      Eskelinen, E.L., et al. 2002. Traffic 3, 878.
      Petiot, A., et al. 2000. J. Biol. Chem. 275, 992.
      Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA 79, 1889.

      Technical Info

      Title
      LentiBrite™ Lentiviral Biosensors for Fluorescent Cellular Imaging: Analysis of Autophagosome Formation
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-May-2012 JSW
      Synonyms3-Methyladenine
      DescriptionA widely used cell-permeable autophagic sequestration blocker that effectly protects cerebellar granule cells from apoptosis following serum/potassium deprivation. Although a known class III PI3K inhibitor (IC50 = 4.5 mM in cell-free enzymatic assays), 3-MA exhibits quite distinct pharmacological properties from those of two other PI3K inhibitors, LY 294002 (Cat. Nos. 440202 and 440204) and Wortmannin (Cat. No. 681675)
      FormWhite to faint yellow powder
      Intert gas (Yes/No) Packaged under inert gas
      CAS number5142-23-4
      Chemical formulaC₆H₇N₅
      Structure formulaStructure formula
      Purity≥95% by HPLC
      SolubilityDMSO (10 mg/ml) or H₂O (5 mg/ml). In applications where high working concentrations are necessary (e.g. 10 mM), the use of a pre-made stock solution may result in the introduction of higher than desired solvent (e.g., DMSO) into the experimental systems. In such cases, it is recommended that the solid compound be weighed out at the time of use and reconstituted directly into the solutions used in the experimental system (buffers, media, etc.). Vortexing may be required for complete solubilization in water.
      Storage Protect from light
      +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C. Stock solutions are stable for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesCanu, N., et al. 2005. J. Neurochem. 92, 1228.
      Hirosako, K., et al. 2004. Biochem. Biophys. Res. Commun. 316, 845.
      Beugnet, A., et al. 2003. Biochem. J. 372, 555.
      Eskelinen, E.L., et al. 2002. Traffic 3, 878.
      Petiot, A., et al. 2000. J. Biol. Chem. 275, 992.
      Seglen, P.O. and Gordon, P.B., 1982. Proc. Natl. Acad. Sci. USA 79, 1889.