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662099 Anti-Ubiquitin Rabbit pAb

This Anti-Ubiquitin Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry, Immunoprecipitation for the detection of Ubiquitin.

Anti-Ubiquitin Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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662099
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
B, Ca, Ch, Dr, E. coli, Fg, Gp, Ht, H, Mk, M, Rb, R, Sh, YeastRbPolyclonal Antibody

Pricing & Availability

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662099-50UL
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      		 Plastic ampoule 50 ul
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      Description
      OverviewRecognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
      Catalogue Number662099
      Brand Family Calbiochem®
      Application Data
      Detection of ubiquitin protein by immunoblotting. Sample: Ubiquitin protein. Primary antibody: Anti-Ubiquitin Rabbit pAb (Cat. No. 662099) (1:5000). Detection: chemiluminescence.
      References
      ReferencesJungmann, J., et al. 1993. Nature 361, 369.
      Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem. 61, 761.
      Hochstrasser, M. 1992. Curr. Opin. Cell Biol. 4, 1024.
      Jentsch, S. 1992. Annu. Rev. Genet. 26, 179.
      Hershko, A., et al. 1982. J. Biol. Chem. 257, 13964.
      Product Information
      FormLiquid
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Immunoprecipitation
      Application NotesImmunoblotting (1:1000, chemiluminescence)
      Immunohistochemistry (1:200)
      Immunoprecipitation (1:100)
      Application CommentsVariables associated with assay conditions will dictate the optimal working dilution.
      Biological Information
      Immunogenbovine erytherocyte ubiquitin conjugated to KLH
      ImmunogenBovine
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Bovine
      • Canine
      • Chicken
      • Drosophila
      • E. coli
      • Frog
      • Guinea Pig
      • Hamster
      • Human
      • Monkey
      • Mouse
      • Rabbit
      • Rat
      • Sheep
      • Yeast
      Antibody TypePolyclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      662099-50UL 04055977261189

      Documentation

      Anti-Ubiquitin Rabbit pAb SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Ubiquitin Rabbit pAb Certificates of Analysis

      TitleLot Number
      662099

      References

      Reference overview
      Jungmann, J., et al. 1993. Nature 361, 369.
      Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem. 61, 761.
      Hochstrasser, M. 1992. Curr. Opin. Cell Biol. 4, 1024.
      Jentsch, S. 1992. Annu. Rev. Genet. 26, 179.
      Hershko, A., et al. 1982. J. Biol. Chem. 257, 13964.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-August-2010 RFH
      ApplicationImmunoblotting (1:1000, chemiluminescence)
      Immunohistochemistry (1:200)
      Immunoprecipitation (1:100)
      Application Data
      Detection of ubiquitin protein by immunoblotting. Sample: Ubiquitin protein. Primary antibody: Anti-Ubiquitin Rabbit pAb (Cat. No. 662099) (1:5000). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody supplied as undiluted serum. Recognizes the ~8.6 kDa unconjugated and various forms of conjugated ubiquitin.
      BackgroundUbiquitin is a highly conserved protein that functions in intracellular regulation. The ubiquitin system is the major pathway for selective protein degradation and is responsible for the degradation of abnormal and short-lived proteins. In the first step, ubiquitin is activated in an ATP-dependent reaction by ubiquitin-activating enzyme (E1). Activated ubiquitin is transferred to a ubiquitin conjugating enzyme (E2), then transferred to a protein substrate with the aid of ubiquitin-protein ligase (E3). An isopeptide linkage is formed between the C-terminus of the ubiquitin and an internal lysine residue of the substrate protein. Degradation requires the presence of a multi-ubiquitin chain on a substrate, which targets the protein for degradation by the 26S proteasome. Ubiquitin molecules are then released and recycled.
      HostRabbit
      Immunogen speciesBovine
      Immunogenbovine erytherocyte ubiquitin conjugated to KLH
      IsotypeIgG
      SpeciesDrosophila, E. coli, bovine, canine, chicken, frog, guinea pig, hamster, human, monkey, mouse, rabbit, rat, sheep, yeast
      FormLiquid
      Preservative≤0.1% sodium azide
      CommentsVariables associated with assay conditions will dictate the optimal working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C)
      Toxicity Standard Handling
      ReferencesJungmann, J., et al. 1993. Nature 361, 369.
      Hershko, A., and Ceichanover, A. 1992. Annu. Rev. Biochem. 61, 761.
      Hochstrasser, M. 1992. Curr. Opin. Cell Biol. 4, 1024.
      Jentsch, S. 1992. Annu. Rev. Genet. 26, 179.
      Hershko, A., et al. 1982. J. Biol. Chem. 257, 13964.