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04-1014 Anti-STAT3 Antibody, clone E121-21, rabbit monoclonal

Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Transcription Factors Gene Symbol: APRF, FLJ20882, HIES, MGC16063 UniProt Number: P40763
04-1014
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICC, IH(P), IPRbUnpurifiedMonoclonal Antibody
      Description
      Catalogue Number04-1014
      DescriptionAnti-STAT3 Antibody, clone E121-21, rabbit monoclonal
      Alternate Names
      • Acute-phase response factor
      • DNA-binding protein APRF
      • signal transducer and activator of transcription 3
      • signal transducer and activator of transcription 3
        (acute-phase response factor)
      Background InformationSTAT proteins (Signal Transduction and Activators of Transcription) are latent cytoplasmic transcription factors that have the dual function of signal transduction and activation of transcription. STATs are activated by tyrosine phosphorylation in response to different ligands, after which they translocate to the cell nucleus. The N-terminal region is highly homologous among the STAT proteins and surrounds a completely conserved arginine residue. STATs are a part of the JAK-STAT signaling pathway – a major pathway of the immune system. All cytokines transduce critical signals through this pathway. STAT3 has been shown to be activated by IFN alpha but not IFN beta. The transcription factors associated with STAT3 are cJun and cyclic AMP responsive enhancer binding protein (CREB). Deletion of the STAT3 gene in knock out mice was lethal at the early embryonic stage.
      References
      Product Information
      FormatUnpurified
      HS Code3002 15 90
      Control
      • A431 cell lysate
      PresentationRabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.
      Applications
      ApplicationPlease note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesImmunohistochemistry Analysis: A 1:50 dilution from a previous lot detected STAT3 in lung squamous carcinoma tissue.
      Immunocytochemistry Analysis: A 1:100 dilution from a previous lot was used in IC.
      Immunoprecipitation Analysis: A 1:20 dilution from a previous lot was used in IP.
      Biological Information
      ImmunogenSynthetic peptide corresponding to residues surrounding Ser727 of human STAT3.
      EpitopeSer727
      CloneE121-21
      HostRabbit
      SpecificityThe antibody only detects STAT3 without phosphorylation on Ser727. It does not detect Ser727-phosphorylated STAT3.
      IsotypeIgG
      Species Reactivity
      • Human
      Species Reactivity NoteHuman. Predicted to cross-react with bovine, and horse, based on sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APRF
      • FLJ20882
      • HIES
      • MGC16063
      Purification MethodUnpurified
      UniProt Number
      UniProt SummaryFUNCTION: Transcription factor that binds to the interleukin-6 (IL-6)-responsive elements identified in the promoters of various acute-phase protein genes. Activated by IL31 through IL31RA.
      SUBUNIT STRUCTURE: Forms a homodimer or a heterodimer with a related family member (at least STAT1). Interacts with NCOA1, PELP1, SOCS7 and STATIP1. Interacts with HCV core protein. Interacts with IL23R in presence of IL23. Interacts with IL31RA. Interacts with SIPAR. Interacts (via SH2 domain) with NLK By similarity. Interacts with KPNA4 and KPNA5; KPNA4 may be the primary mediator of nuclear import By similarity. Interacts with TMF1.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note: Shuttles between the nucleus and the cytoplasm. Constitutive nuclear presence is independent of tyrosine phosphorylation.
      TISSUE SPECIFICITY: Heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas.
      PTM: Tyrosine phosphorylated in response to IL-6, IL-11, CNTF, LIF, CSF-1, EGF, PDGF, IFN-alpha and OSM. Phosphorylated on serine upon DNA damage, probably by ATM or ATR. Serine phosphorylation is important for the formation of stable DNA-binding STAT3 homodimers and maximal transcriptional activity.
      INVOLVEMENT IN DISEASE: Defects in STAT3 are the cause of hyperimmunoglobulin E recurrent infection syndrome autosomal dominant (AD-HIES) [MIM:147060]; also known as hyper-IgE syndrome or Job syndrome. AD-HIES is a rare disorder of immunity and connective tissue characterized by immunodeficiency, chronic eczema, recurrent Staphylococcal infections, increased serum IgE, eosinophilia, distinctive coarse facial appearance, abnormal dentition, hyperextensibility of the joints, and bone fractures.
      MISCELLANEOUS: Involved in the gp130-mediated signaling pathway.
      SEQUENCE SIMILARITIES: Belongs to the transcription factor STAT family.
      Contains 1 SH2 domain.
      Molecular Weight~92 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot on A431 cell lysates.
      Western Blot Analysis: A 1:1,000 dilution of this antibody was used to detect STAT3 in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      04-1014 04053252729881

      Documentation

      Anti-STAT3 Antibody, clone E121-21, rabbit monoclonal SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-STAT3 Antibody, clone E121-21, rabbit monoclonal Certificates of Analysis

      TitleLot Number
      Anti-STAT3, clone E121-21, Rabbit Monoclonal - 2377648 2377648
      Anti-STAT3, clone E121-21, Rabbit Monoclonal - 2161140 2161140
      Anti-STAT3, clone E121-21, Rabbit Monoclonal - NG1628716 NG1628716
      Anti-STAT3, clone E121-21, Rabbit Monoclonal - NG1675978 NG1675978
      Anti-STAT3, clone E121-21, Rabbit Monoclonal - NG1726968 NG1726968
      Anti-STAT3, clone E121-21, Rabbit Monoclonal - NG1823954 NG1823954

      Brochure

      Title
      Advancing cancer research: From hallmarks & biomarkers to tumor microenvironment progression

      Technical Info

      Title
      JAK/STAT Signaling Research Focus

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies