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MABC199 Anti-BRCA1 Antibody, clone MS110

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MABC199
100 µg  
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      Overview

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC199
      DescriptionAnti-BRCA1 Antibody, clone MS110
      Alternate Names
      • Breast cancer type 1 susceptibility protein
      • RING finger protein 53
      Background InformationBreast cancer type 1 susceptibility protein (BCRA1; RING finger protein 53) functions as a tumor suppressor. BRCA1 is an important member of the DNA repair pathway. After DNA damage, BRCA1 is phosphorylated on multiple serine residues by the ATR kinase or Chk2, and is localized to lesion sites with PCNA. BRCA1 interacts with a wide range of proteins including Rad50, NBS1, and Mre11, c-Abl tyrosine kinase, and γH2A.X, involved in the detection of damaged DNA and activation of appropriate repair pathways. BRCA1 may also respond to DNA damage by promoting gene expression through association with transcriptional proteins and RNA polymerase II, and may regulate the p53 pathway by this mechanism. Previous studies have indicated that mutations in the BRCA1 gene may contribute to the development of breast and ovarian cancer.
      Product Information
      FormatPurified
      Control
      • HeLa nuclear extract
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-BRCA1 Antibody, clone MS110 is an antibody against BRCA1 for use in Western Blotting, ICC.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:500 dilution of this antibody detected in BRCA1 in MCF7 cells.

      Immunoprecipitation Analysis: A representative lot from an independent laboratory immunoprecipitated BRCA1 in IP (Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).

      Immunohistochemistry Analysis: A representative lot from an independent laboratory detected BRCA in ovarian cancer and breast cancer tissues (Scully, R., et al. (1996). Science. 272(5258):123-126; Wilson, C. A., et al. (1999). Nat Genet. 21(2):236-240.).

      Immunofluorescence Analysis: A representative lot from an independent laboratory detected BRCA1 in a variety of certain cell lines (Scully, R., et al. (1996). Science. 272(5258):123-126.).
      Biological Information
      ImmunogenRecombinant protein corresponding to the N-terminus of human BRCA1.
      EpitopeN-terminus
      CloneMS110
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityThis antibody recognizes the N-terminus of BRCA1.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • BRCA1
      • RNF53
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~220 kDa and ~85 kDa observed. Multiple isoforms exist at various molecular weights and may be subject to post-translational modification.
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa nuclear extract.

      Western Blot Analysis: 1 µg/mL of this antibody detected BRCA1 in 10 µg of HeLa nuclear extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Global Trade Item Number
      Catalogue Number GTIN
      MABC199 04053252597022