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Pluripotent Mouse ES Cells

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Pluripotent Mouse ES Cells in culture
Featured Product:
MilliTrace™ Nanog GFP Reporter Mouse ES Cells
Mouse ES cells with a GFP reporter
The MilliTrace™ Nanog GFP reporter mouse embryonic stem cells express green fluorescent protein (GFP) under the control of the mouse Nanog promoter. These cells provide a quick, non-invasive method by which to monitor the expression of Nanog in pluripotent embryonic stem cells. The MilliTrace™ reporter cell lines are provided in kits containing 1X10^6 viable cells and 500 mL optimized expansion medium to help maintain expression of the transgene. Cells are adapted for feeder-free culture on gelatin coated plasticware.
The development of transgenic and gene knockout technology has provided an effective tool for the analysis of gene function. These targeting experiments commonly use murine embryonic stem (ES) cells, cultured in vitro. As a result, efficient procedures for the in vitro culture and maintenance of pluripotent ES cells have been vital to the success of this research. Merck’s range of ES cell qualified products provides researchers with convenient and cost effective solutions for the reliable culture of ES cells.

Merck provides the largest and most comprehensive range of products for your mouse ES cell culture needs. Highlights include unique mLIF formulations such as ESGRO, a medium supplement for the maintenance of pluripotent mouse ES cells; PMEF feeder cells, for both mouse and human ES cell culture support; ESGRO Complete Plus, a serum-free system for the maintenance and derivation of mouse ES cell lines in the absence of FBS and feeder cells; and RESGRO, a culture medium for the rescue of partially differentiated ES cell lines and improved ES cell derivation.

Mouse Embryonic Stem Cell Lines

Merck offers a wide selection of mouse ES cells derived from mice of different genetic backgrounds. The PluriStem® range of cells were derived from inbred strains of mice and are useful for modeling genetic diversity in directed differentiation studies and drug screening bioassays. Additionally, Merck provides EmbryoMax® mouse ES cells, established lines for gene targeting experiments. These lines have high targeting efficiencies and have achieved germline transmission through multiple experiments.