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Dot/Slot (Filtration) Blotting | Life Science Research | Merck
The possible causes and potential remedies for challenges encountered dot/slot blotting, which uses vacuum filtration to transfer protein onto a microporous membrane. -
Sample Preparation and Gel Electrophoresis | Life Science Research | Merck
The possible causes and potential remedies for challenges encountered during preparation of samples for SDS-PAGE (sodium dodecyl sulfate polyacrylamide gel electrophoresis) and optimizing electrophoresis conditions. -
Western Blotting Protocols | Life Science Research | Merck
Review Western blotting protocols on various applications including rapid and standard immunodetection, peptide inhibition and membrane stripping. -
Preparing cell lysates | Life Science Research | Merck
This page describes common challenges encountered when lysing cells and extracting proteins prior to Western blotting. Total protein concentration must be determined for these cell lysates. Variables affecting each of these steps are outlined below, as each could affect the sensitivity and reproducibility of the Western blot. -
Immunodetection | Life Science Research | Merck
The possible causes and potential remedies for challenges encountered as a result of blocking, washing, antibody incubation, and detection/exposure of Western blots. -
Western Blotting | Life Science Research | Merck
We provide a wide portfolio of western blotting products including optimized reagents for chemiluminescent and fluorescent Western blots, and the SNAP i.d.® 2.0 system. -
Troubleshooting Western Blots | Life Science Research | Merck
An online troubleshooting guide describing the most common symptoms of Western blots that exhibit poor signal-to-noise, poor reproducibility, or other obstacles to interpretation. Choose a topic to view possible causes and potential remedies. -
Western Blotting Applications Notes | Life Science Research | Merck
Discover updated application notes for Western blotting topics and find useful insights for antibody reuse, triple well blotting, and immunodetection applications. -
LentiBrite™ LC3-II Enrichment Kit | Life Science Research | Merck
LC3-II enrichment Kit (Western Blot) enables quantification of autophagosome density by utilizing a permeabilization procedure that reveals quantities of LC3-II, without interference from LC3-I, by Western blotting analysis.