Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

69659 pLysS DNA - Novagen

pLysS DNA - Novagen : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
69659
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
69659-3 Flacon en verre 1 μg
Description
OverviewpLysS and pLysE are 4886bp plasmids constructed by insertion of the T7 lysozyme gene into the BamH I site of pACYC184 (1). These plasmids are not cloning vectors; they are used in λDE3 lysogenic hosts to suppress basal expression from the T7 promoter by producing T7 lysozyme, a natural inhibitor of T7 RNA polymerase. The two plasmids differ only by the orientation of the T7 lysozyme gene. In pLysS the T7 lysozyme coding sequence is in the antisense orientation relative to the tet promoter, so only a small amount of T7 lysozyme is produced. In pLysE large amounts of T7 lysozyme are produced from the tet promoter. The construct also contains the weak T7 φ3.8 promoter immediately following the lysozyme gene. The p15A origin of replication is compatible with those found in pBR322- and pUC-derived plasmids.

This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license.





This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
Catalogue Number69659
Brand Family Novagen®
References
References1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
69659-3 04055977274479

Documentation

pLysS DNA - Novagen FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

pLysS DNA - Novagen Certificats d'analyse

TitreNuméro de lot
69659

Références bibliographiques

Aperçu de la référence bibliographique
1. Studier , F.W. (1991) J. Mol. Biol. 219, 37-44.

Citations

Titre
  • Pernilla Larsson Birgander, et al. (2005) Nucleotide-dependent formation of catalytically competent dimers from engineered monomeric ribonucleotide reductase protein R1. Journal of Biological Chemistry 280, 14997-15003.
  • Dale K. Shumaker, et al. (2005) Functions and dysfunctions of the nuclear lamin Ig-fold domain in nuclear assembly, growth, and Emery-Dreifuss muscular dystrophy. Proceedings of the National Academy of Sciences (USA) 102, 15494-15499.
    		•

    Carte des vecteurs

    Titre
    TB107 pLysS and pLysE Plasmid Map

    Séquence du vecteur

    Titre
    pLysS Sequence

    Produits & Applications associés

    Related Products

    Référence Description
    69418 pET-3a DNA - Novagen
    69421 pET-3d DNA - Novagen
    69431 pET-9a DNA - Novagen
    69434 pET-9d DNA - Novagen
    69660 pET-14b DNA - Novagen
    69661 pET-15b DNA - Novagen
    69662 pET-16b DNA - Novagen
    69663 pET-17b DNA - Novagen
    69677 pET-19b DNA - Novagen
    69739 pET-20b(+) DNA - Novagen
    Show more

    Catégories

    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors