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69740 pET-21a(+) DNA - Novagen

69740
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RéférenceConditionnement Qté
69740-3 Flacon en verre 10 μg
Description
OverviewThe pET-21a-d(+) vectors carry an N-terminal T7•Tag® sequence plus an optional C-terminal His•Tag® sequence. These vectors differ from pET-24a-d(+) only by their selectable marker (ampicillin vs. kanamycin resistance). Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the vector map (TB036 ). The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand.

The pET Vectors are supplied as purified plasmid DNA (10 µg). Each order of pET DNA also includes an Induction Control strain (supplied as a glycerol stock). Please contact technical service if you need additional information.




This product is sold for internal research use only. Any commercial use of this product, its components, and/or any derivatives thereof (including but not limited to proteins produced using the product or its components) (together and hereinafter the 'EMD Product') requires signature of a written commercial use agreement with EMD Millipore Corporation or its successor-in-interest. Commercial use shall include but not be limited to: (1) use of the EMD Product to manufacture products for sale to third parties; (2) use of the EMD Product to provide services, information, or data to third parties in exchange for consideration; (3) use of the EMD Product for therapeutic, diagnostic or prophylactic purposes (including as part of a device, chip, assay or other product); or (4) resale of the EMD Product, whether or not such EMD Product is resold for research use. Nothing contained herein shall be deemed to represent or warrant that additional third party rights are not required for use of the EMD Product. Please direct any questions on these use restrictions to: licensing@milliporesigma.com.
This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges MilliporeSigma to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
Catalogue Number69740
Brand Family Novagen®
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
69740-3 07790788052652

Documentation

pET-21a(+) DNA - Novagen Certificats d'analyse

TitreNuméro de lot
69740

Citations

Titre
  • Brian P. Anton, et al. (2008) RimO, a MiaB-like enzyme, methylthiolates the universally conserved Asp88 residue of ribosomal protein S12 in Escherichia coli. Procedings of the National Academy of Science 105, 1826-1831.
  • Mateen A. Khan, et al. (2008) Potyvirus genome-linked protein, VPg, directly affects wheat germ in vitro translation: interactions with translation initiation factors eIF4F and EIFiso4F. Journal of Biological Chemistry 283, 1340-1349.
  • Ian T. Major and C. Peter Constabel. (2008) Functional analysis of the Kunitz trypsin inhibitor family in poplar reveals miochemical diversity and multiplicity in defense against herbivores. Plant Physiology 146, 888-903.
  • Monika Walter, et al. (2008) Structure of the receptor-binding protein of bacteriophage Det7: a podoviral tail spike in a myovirus. Journal of Virology 82, 2265-2273.
  • Daniela A. Geisler, et al. (2007) Ca2+-binding and Ca2+-independent respiratory NADH and NADPH dehydrogenases of Arabidopsis thaliana. Journal of Biological Chemistry 282, 28455-28464.
  • Hiroko Hayakawa, et al. (2007) Soluble ST2 blocks interleukin-33 signaling in allergic airway inflammation. Journal of Biological Chemistry 282, 26369-26380.
  • Fan Yang, et al. (2007) Solution structure and backbone dynamics of an endopeptidase HycI from Escherichia coli: implications for mechanism of the NiFe hydrogenase maturation. Journal of Biological Chemistry 282, 3856-3863.
  • Marielle E. Yohe, et al. (2007) Auto-inhibition of the Dbl family protein Tim by an N-terminal helical motif. Journal of Biological Chemistry 282, 13813-13823.
  • Hyesook Yoon, et al. (2007) Activation profiles and regulatory cascades of the human kallikrein-related peptidases. Journal of Biological Chemistry 282, 31852-31864.
  • Andrew D. Ferguson, et al. (2006) NMR structures of the selenoproteins sep15 and selM reveal redox activity of a new thioredoxin-like family. Journal of Biological Chemistry 281, 3536-3543.
  • Martin Gengenbacher, et al. (2006) Vitamin B6 biosynthesis by the malaria parasite Plasmodium falciparum: biochemical and structural insights. Journal of Biological Chemistry 281, 3633-3641.
  • Chad E. Metcalf and David A. Wassarman. (2006) DNA binding properties of TAF1 isoforms with two AT-hooks. Journal of Biological Chemistry 281, 30015-30023.
  • James F. Parsons, et al. (2006) Crystallization and X-ray diffraction analysis of salicylate synthase, a chorismate-utilizing enyme involved in siderophore biosynthesis. Acta Crystallographica F62, 271-274.
  • Jonathan D. Dattelbaum, et al. (2005) Analysis of allosteric signal transduction mechanisms in an engineered fluorescent maltose biosensor. Protein Science 14, 284-291.
  • Luca Federici, et al. (2005) The crystal structure of the outer membrane protein vceC from the bacterial pathogen Vibrio cholerae at 1.8 Å resolution. Journal of Biological Chemistry 15307-15314.
  • Nikhil A. Gokhale, et al. (2005) Phosphoinositide specificity of and mechanism of lipid domain formation by annexin A2-p11 heterotetramer. Journal of Biological Chemistry 280, 42831-42840.
  • Seung-Soon Im, et al. (2005) Glucose-stimulated upregulation of GLUT2 gene is mediated by sterol response element-binding protein-1c in the hepatocytes. Diabetes 54, 1684-1691.
  • Hironori Kawakami, Kenji Keyamura and Tsutomu Katayama. (2005) Formation of an ATP-DnaA-specific initiation complex requires DnaA arginine-285, a conserved motif in the AAA+ protein family. Journal of Biological Chemistry 280, 27420-27430.
  • Vyacheslav M. Labunskyy, et al. (2005) A novel cysteine-rich domain of SEP15 mediates the interactions with UDP-glucose: Glycoprotein glucosyltransferase. Journal of Biological Chemistry 280, 37839-37845.
  • Ayman Marei, et al. (2005) Superior T cell activation by ESAT-6 as compared with the ESAT-6-CFP-10 complex. International Immunology 17, 1439-1446.
  • Woo-Hyun Park, et al. (2005) Direct DNA binding activity of the fanconi anemia D2 protein. Journal of Biological Chemistry 280, 23593-23598.
  • C. Michael Reynolds, et al. (2005) A phosphoethanolamine transferase specific for the outer 3-deoxy-D-manno-octulosonic acid residue of Escherichia coli lipopolysaccharide. identification of the eptB gene and Ca++ hypersensitivity of an eptB deletion mutant. Journal of Biological Chemistry 280, 21202-21211.
  • Roberta Sacchetto, et al. (2005) Glycogen- and PP1c-targeting subunit GM is phosphorylated at SER48 by sarcoplasmic reticulum-bound CaMKII in rabbit fast-twitch skeletal muscle. Journal of Biological Chemistry 280, 7147-7155.
  • J. Jefferson Smith, et al. (2005) Orthogonal site-specific protein modification by engineering reversible thiol protection mechanisms. Protein Science 14, 64-73.
  • Wolfdieter Springer, et al. (2005) A Caenorhabditis elegans Parkin mutant with altered solubility couples α-synuclein aggregation to proteotoxic stress. Human Molecular Genetics 14, 3407-3423.
  • Yoshimitsu Takahashi and Yoshiko Kikuchi. (2005) Yeast PIAS-type Ull1/Siz1 is composed of SUMO ligase and regulatory domains. Journal of Biological Chemistry 280, 35822-35828.
  • Marina Tambasco-Studart, et al. (2005) Vitamin B6 biosynthesis in higher plants. Proceedings of the National Academy of Sciences (USA) 102, 13687-13692.
  • Paul R. Wheeler, et al. (2005) Functional demonstration of reverse transsulfuration in the Mycobacterium tuberculosis complex reveals that methionine is the preferred sulfur source for pathogenic Mycobacteria. Journal of Biological Chemistry 280, 8069-8078.
  • Saloni Yatin Pasta, et al. (2004) The IXI/V motif in the C-terminal extension of a-crystallins: alternative interactions and oligomeric assemblies. Molecular Vision 10, 655-662.
  • Fabio Re and Jack L. Strominger. (2004) IL-10 Released by Concomitant TLR2 Stimulation Blocks the Induction of a Subset of Th1 Cytokines That Are Specifically Induced by TLR4 or TLR3 in Human Dendritic Cells. Journal of Immunology 173, 7548-7555.
  • Yukinobu Uchiyama, et al. (2004) Plant DNA polymerase k, a DNA repair enzyme that functions in plantmeristematic and meiotic tissues. European Journal of Biochemistry 271, 2799-2807.
  • Michael H. Kagey, Tiffany A. Melhuish and David Wotton. (2003) The polycomb protein Pc2 is a SUMO E3. Cell 113, 127-137.
  • Philip R. Renshaw, et al. (2002) Conclusive evidence that the major T-cell antigens of the Mycobacterium tuberculosis complex ESAT-6 and CFP-10 form a tight, 1:1 complex and characterization of the structural properties of ESAT-6, CFP-10, and the ESAT-6-CFP-10 complex. Journal of Biological Chemistry 277, 21598-21603.
  • Jennifer S. Laurence, et al. (2000) CC Chemokine MIP-1 can function as a monomer and depends on Phe13 for receptor binding. Biochemistry 39, 3401-3409.
  • E.B. Fauman, et al. (1998) Crystal structure of the catalytic domain of the human cell cycle control phosphatase, Cdc25A. Cell 93, 617-625.
  • Jamal A. Ibdah, et al. (1998) Mild trifunctional protein deficiency is associated with progressive neuropathy and myopathy and suggests a novel genotype- phenotype correlation. Journal of Clinical Investigation 102, 1193-1199.
  • Junmin Peng, Nick F. Marshall and David H. Price. (1998) Identification of a cyclin subunit required for the function of Drosophila P-TEFb. Journal of Biological Chemistry 273, 13855-13860.
  • Robert M. Brazas, et al. (1995) Determining the requirements for cooperative DNA binding by Swi5p and Pho2p (Grf10p/Bas2p) at the HO promoter. Journal of Biological Chemistry 270, 29151-29161.
  • Brian L. Webb, Michael M. Cox and Ross B. Inman. (1995) An interaction between the Escherichia coli RecF and RecR proteins dependent on ATP and double-stranded DNA. Journal of Biological Chemistry 270, 31397-31404.
  • Protocoles Utilisateur

    Titre
    TB053 Academic and Non-profit Laboratory Assurance Letter
    TB055 pET System Manual
    TB555, Mitochondrial Complex II Activity Assay

    Carte des vecteurs

    Titre
    TB036VM pET-21a-d(+) Vector Map

    Produits & Applications associés

    Related Products

    Catégories

    Life Science Research > Genomic Analysis > Transfection and Protein Expression > Bacterial Expression > Bacterial Expression Vectors