Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

14-733 c-Kit (V654A) Protein, active, 10 µg

V654A is an acquired resistance mutation found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance & rapid progession of GISTs.

c-Kit (V654A) Protein, active, 10 µg : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
14-733
10 µg  
Purchase on Sigma-Aldrich

Aperçu

Replacement Information
Description
Catalogue Number14-733
Brand Family Chemicon®
Trade Name
  • Chemicon
Descriptionc-Kit (V654A) Protein, active, 10 µg
OverviewN-terminal GST tagged, recombinant human cKit, amino acids 544 - end containing the V654A mutation. Co-ordinates encompass the cytoplasmic domain (544-976) according in Swiss Prot P10721. V654A is an acquired resistance mutation which has been found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance and rapid progession of GISTs. (Chen LL et al., Cancer Research, (2004); 64:5913-5919).
References
Product Information
Quality LevelMQ100
Applications
ApplicationV654A is an acquired resistance mutation found in patients with gastrointestinal stromal tumours (GISTs) undergoing treatment with Imatinib. The V654A mutation is strongly correlated with imatinib resistance & rapid progession of GISTs.
Key Applications
  • Kinase Assay
Biological Information
Sourcerecombinant protein expressed in Sf21 cells
Specific Activity169 U/mg, where one unit of cKit (V654A) activity is defined as 1nmol phosphate incorporated into 250 μM GGMEDIYFEFMGGKKK per minute at 30 °C with a final ATP concentration of 100 μM.
Entrez Gene Number
Entrez Gene SummaryThis gene encodes the human homolog of the proto-oncogene c-kit. C-kit was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. This protein is a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). Mutations in this gene are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous lukemia, and piebaldism. Multiple transcript variants encoding different isoforms have been found for this gene.
Gene Symbol
  • KIT
  • C-Kit
  • PBT
  • CD117
  • SCFR
  • c-kit
Protein Targetc-Kit
Purification MethodPurified using glutathione-agarose. Purity 99% by SDS-PAGE and Coomassie blue staining.
Target Sub-FamilyTK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P10721 # This is the receptor for stem cell factor (mast cell growth factor). It has a tyrosine-protein kinase activity. Binding of the ligands leads to the autophosphorylation of KIT and its association with substrates such as phosphatidylinositol 3-kinase (Pi3K).
SIZE: 976 amino acids; 109865 Da
SUBUNIT: Interacts with APS. Interacts with the tenth PDZ domain of MPDZ.
SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
DISEASE: SwissProt: P10721 # Defects in KIT are a cause of piebaldism [MIM:172800]. Piebaldism is an autosomal dominant genetic developmental abnormality of pigmentation characterized by congenital patches of white skin and hair that lack melanocytes. & Defects in KIT are a cause of gastrointestinal stromal tumor (GIST) [MIM:606764]. & Defects in KIT have been associated with testicular tumors [MIM:273300]. It includes germ cell tumor (GCT) or testicular germ cell tumor (TGCT).
SIMILARITY: SwissProt: P10721 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily. & Contains 5 Ig-like C2-type (immunoglobulin-like) domains. & Contains 1 protein kinase domain.
Molecular Weight76.7kDa.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 250 μM GGMEDIYFEFMGGKKK.
Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of active cKit (V654A).
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 6 months at -70 °C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70 °C.
Packaging Information
Material Size10 µg
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
14-733 04053252513480

Produits & Applications associés

Alternative Packsize

Référence Description
14-733M c-Kit (V654A) Protein, active, 250 µg

Familles de produits

Kinase & Phosphatase Products

Millipore offers a large portfolio of Kinases, Phophatases, Substrates, Inhibitors, Kits, reagents & tools for Signaling research. See below for a list of these products, in multiple pack sizes.
En savoir plus >>

Catégories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases