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14-608M ZIPK Protein, active, 250 µg

Active, N-terminal, GST tagged, recombinant, of human ZIPK amino acids 1-290, for use in Kinase Assays.

14-608M
250 µg  Also available in 10 μg size (cat#14-608) and in bulk (cat#14-608-K).
Purchase on Sigma-Aldrich

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Replacement Information
Description
Catalogue Number14-608M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionZIPK Protein, active, 250 µg
OverviewN-terminal, GST tagged, recombinant, of human ZIPK amino acids 1-290
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal, GST tagged, recombinant, of human ZIPK amino acids 1-290, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in E. coli
Entrez Gene Number
Entrez Gene SummaryDeath-associated protein kinase 3 (DAPK3) induces morphological changes in apoptosis when overexpressed in mammalian cells. These results suggest that DAPK3 may play a role in the induction of apoptosis.
Gene Symbol
  • DAPK3
  • FLJ36473
  • ZIP-kinase
  • ZIPK
  • Dlk
  • ZIP
Protein TargetZIPK
Purification MethodGlutathione agarose affinity chromatography
Target Sub-FamilyCAMK
UniProt Number
UniProt SummaryFUNCTION: SwissProt: O43293 # Serine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis.
COFACTOR: Magnesium.
SIZE: 454 amino acids; 52536 Da
SUBUNIT: Homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. Also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. Interacts with AATF and CDC5L.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm (By similarity). Note=Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Associates to centromeres from prophase to anaphase.
SIMILARITY: SwissProt: O43293 ## Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily. & Contains 1 protein kinase domain.
Molecular Weight64.3kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 250μM ZIPtide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-608) and in bulk (cat#14-608-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
14-608M 04053252924255

Documentation

Produits & Applications associés

Alternative Packsize

Référence Description
14-608 ZIPK Protein, active, 10 µg

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Catégories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases