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110275 Gélose VRBG (cristal violet rouge neutre sels bilièe glucose)

110275
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Valeur de pHSolubilité
7.3 (40 g/l, H₂O, 37 °C) (après autoclavage)40 g/l

Products

RéférenceConditionnement Qté
1102750500 Flacon plastique 500 g
Description
Référence110275
SynonymesCrystal-violet neutral-red bile dextrose agar, VRBD-agar (Crystal-violet neutral-red bile dextrose agar) acc. to MOSSEL
AperçuGranuCult™ is our new brand name for our existing granulated dehydrated culture media which apply to new EN ISO 11133:2014 and various other ISO standards and FDA-BAM / USDA-FSIS methods for testing of food and water samples. All these media are currently being updated to be fully compliant to the latest version of these standards and regulations with respect to composition and quality control and will be available shortly.
Références bibliographiques
Informations produit
Code SH3821 00 00
Quality LevelMQ200
Applications
Informations biologiques
Informations physico-chimiques
Valeur de pH7.3 (40 g/l, H₂O, 37 °C) (après autoclavage)
Densité720 kg/m3
Solubilité40 g/l
Dimensions
Informations sur les matériaux
Informations toxicologiques
Informations de sécurité selon le SGH
Classe de stockage10 - 13 Autres liquides ou matières solides
WGKWGK 1 pollue faiblement l'eau
Élimination3
Les réactifs organiques liquides relativement stables du point de vue chimique sont rassemblés dans le récipient A. S'ils contiennent des halogènes, les mettre dans le récipient B. Résidus solides: récipient C.
Informations sur la sécurité
Notifications sur l'utilisation du produit
Informations relatives au stockage et à l'expédition
StockageConserver de +15°C à +25°C.
Informations sur l'emballage
Informations sur le transport
Information complémentaire
Caractéristiques
Appearance (clearness)clear
Appearance (color)red
Solidification behaviour (4 hrs., 45 °C)liquid
pH-value (25 °C)7.2 - 7.6
Typical composition (g/litre): Peptone from gelatine 7.0; Yeast extract 3.0; Sodium chloride 5.0; D(+)Glucose 10.0; Bile salt mixture 1.5; Neutralred 0.03; Crystal violet 0.002; Agar-agar 13.0.
Growth promotion test in accordance with the current version of harmonised method of EP, USP and JP.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
10 - 100
Inoculum on reference medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))10 - 100
Inoculum (Staphylococcus aureus ATCC 6538 (WDCM 00032))≥ 10000
Inoculum (Bacillus cereus ATCC 11778 (WDCM 00001))≥ 10000
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))
50 - 150 %
Recovery on test medium (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))50 - 150 %
Colony color (Escherichia coli ATCC 8739 (WDCM 00012))red colonies
Colony color (Pseudomonas aeruginosa ATCC 9027 (WDCM 00026))nearly colourless to slightly red colonies
Precipitate (Escherichia coli ATCC 8739 (WDCM 00012))+
Growth (Staphylococcus aureus ATCC 6538 (WDCM 00032))no growth
Growth (Bacillus cereus ATCC 11778 (WDCM 00001))no growth
Incubation:
up to 18 hours at 30-35°C aerobic
Staphylococcus aureus and Bacillus cereus at least 24 hours.
Reference medium: Tryptic Soy Agar
Growth promotion test in accordance with the current version of DIN EN ISO 11133.
Inoculum on reference medium (Escherichia coli ATCC 8739 (WDCM 00012))
Inoculum on reference medium (Escherichia coli ATCC 25922 (WDCM 00013))
Inoculum on reference medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))
Inoculum on reference medium (Salmonella enteritidis ATCC 13076 (WDCM 00030))
Colony count (Escherichia coli ATCC 8739 (WDCM 00012))
Colony count (Escherichia coli ATCC 25922 (WDCM 00013))
Colony count (Salmonella typhimurium ATCC 14028 (WDCM 00031))
Colony count (Salmonella enteritidis ATCC 13076 (WDCM 00030))
Recovery on test medium (Escherichia coli ATCC 8739 (WDCM 00012))
≥ 50 %
Recovery on test medium (Escherichia coli ATCC 25922 (WDCM 00013))≥ 50 %
Recovery on test medium (Salmonella typhimurium ATCC 14028 (WDCM 00031))≥ 50 %
Recovery on test medium (Salmonella enteritidis ATCC 13076 (WDCM 00030))≥ 50 %
Colonies (Escherichia coli ATCC 8739 (WDCM 00012))Pink to red colonies with or without precipitation halo
Colonies (Escherichia coli ATCC 25922 (WDCM 00013))Pink to red colonies with or without precipitation halo
Colonies (Salmonella typhimurium ATCC 14028 (WDCM 00031))Pink to red colonies with or without precipitation halo
Colonies (Salmonella enteritidis ATCC 13076 (WDCM 00030))Pink to red colonies with or without precipitation halo
Growth (Enterococcus faecalis ATCC 29212 (WDCM 00087))total inhibition
Growth (Enterococcus faecalis ATCC 19433 (WDCM 00009))total inhibition
Incubation: 24 ± 2 hours at 37 ± 1 °C
A recovery rate of 50 % is equivalent to a productivity value of 0.5.
The indicated colony counts result from the sum of a triple determination.
Reference medium: Tryptic Soy Agar
The information above is current at this time of publication and is subject to change without notice (except for customers holding a change control agreement with our company). The information/format can be altered at any time and inany way if internal company matters or Standard Regulationsdo say so.
Global Trade Item Number
Référence GTIN
1102750500 04022536123325

Documentation

Gélose VRBG (cristal violet rouge neutre sels bilièe glucose) FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Gélose VRBG (cristal violet rouge neutre sels bilièe glucose) Certificats d'analyse

Référence ConditionnementSpécificationNuméro de lot
1102750500 Flacon plastiqueDocument de spécification PDF

Brochure

Titre
Eine Marke mit dauerhaftem Wert und das sichere Fundament für Ihren Erfolg -Produkte für mikrobiologische Untersuchungen
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Fiche technique

Titre
Data Sheet 1.10275.0500

Produits & Applications associés

Produits associés classés par : Brand Facete

Catégories

Industrial Microbiology > Bioburden Testing > Bioburden Testing for Pharmaceutical & Cosmetics Industry > Test for specified Microorganisms
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Coliforms, E. coli & Enterobacteriaceae
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > E. Sakazakii
Industrial Microbiology > Culture Media > Culture Media for Pharmaceutical & Cosmetics Industry > Dehydrated Culture Media > Dilution, Enrichment & Microbial Enumeration > Environmental Tests
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Product > Dehydrated and Ready-to-use Culture Media
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > Listeria Monocytogenes & Listeria Genus
Industrial Microbiology > Pathogen & Spoilage Testing > Pathogen Detection > Pathogen Detection by Organism > E. Coli O157 & STEC
Industrial Microbiology > Culture Media > Culture Media for Pharmaceutical & Cosmetics Industry > Dehydrated Culture Media > Isolation & Differentiation by Organism > Coliforms, E. coli & Enterobacteriaceae
Industrial Microbiology > Culture Media > Culture Media for Food & Beverage Industry > Dehydrated Culture Media > Enrichment, Isolation & Differentiation by Organism > Listeria