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AC111P Sheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate

Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate, Cat. No. AC111P, is a secondary antibody HRP conjugate, validated for use in Western Blotting application with little or no cross-reactivity toward mouse IgA & IgM, chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Species Reactivity: Mouse
AC111P
500 µL  
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Description
Catalogue NumberAC111P
ReplacesAP300P
DescriptionSheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate
Background InformationImmunoglobulin G (IgG) is part of the family of glycoprotein immunoglobulins which stage immune responses against pathogenic organisms. IgG contributes to the immune response by mobilizing innate immune effector cells and inducing and pro-inflammatory responses. Such proinflammatory responses are mediated by Fc receptors which interact with sialylated Fc fragments on IgG molecules.
References
Product Information
FormatHRP
PresentationPurified sheep polyclonal antibody in PBS with StabilZyme® HRP Conjugate Stabilizer and 0.05% Proclin.
Quality LevelMQ100
Applications
ApplicationSheep Anti-Mouse IgG, Species absorbed, HRP conjugate, Cat. No. AC111P, is a secondary antibody HRP conjugate, validated for use in Western Blotting application with little or no cross-reactivity toward mouse IgA & IgM, chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species.
Key Applications
  • Western Blotting
Application NotesWestern Blotting Analysis: Performance of a representative lot was validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of HeLa cell lysate.
Biological Information
ImmunogenPurified mouse IgG.
ConcentrationPlease refer to lot specific datasheet.
HostSheep
SpecificityBased on ELISA analysis using the unconjugated antibody (Cat. No. AC111), this antibody reacts with whole molecule mouse IgG (IgG2>IgG2a>IgG3>>>IgG1) with minimal or no cross-reactivity toward mouse IgA or IgM.
Species Reactivity
  • Mouse
Species Reactivity NoteMouse. This antibody has been species absorbed and tested by ELISA prior to conjugation to ensure minimal cross-reaction toward chicken IgY or IgG from human, rat, rabbit, guinea pig, and hamster species, but may cross-react with immunoglobulins from other species.
Antibody TypePolyclonal Antibody
Purification MethodAmmonium Sulfate.
Molecular Weight~150.0 kDa observed.
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: Performance of this HRP-conjugated secondary antibody (Cat No. AC111P) is validated at a 1:4,000 dilution for target band labeling following a mouse monoclonal GAPDH antibody (Cat. No. MAB374) in Western blotting application using 10 µg of A431 cell lysate.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
Packaging Information
Material Size500 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
AC111P 04054839052934

Documentation

Sheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Sheep Anti-Mouse IgG Antibody, Species absorbed Antibody, HRP conjugate Certificats d'analyse

TitreNuméro de lot
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 3474111 3474111
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 3596228 3596228
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 3749948 3749948
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 3878279 3878279
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate - 4080463 4080463
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate -Q2735581 Q2735581
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate Polyclonal Antibody 3153211
Sheep Anti-Mouse IgG, Species absorbed, HRP conjugate Polyclonal Antibody 2981025