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Préparation d’échantillons

MultiScreen plates offer diverse and versatile formats for a variety of preparative methods.

Aperçu

Caractéristiques

Guide d'achat

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Plaques de filtration MultiScreenHTS avec membrane Durapore en PVDF hydrophileSupprimer le tri & les filtres Show Filter
RéférenceDimension de poresCode couleurMatériau du dispositifStérilitéConditionnement
MSGVN22100.22 µm Transparent Styrène Non stérile 10
MSGVN22500.22 µm Transparent Styrène Non stérile 50
MSGVS22100.22 µm Transparent Styrène Stérile 10
MSGVN2B500.22 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 50
MSHVN45100.45 µm Transparent Styrène Non stérile 10
MSHVN45500.45 µm Transparent Styrène Non stérile 50
MSHVN4B500.45 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 50
MSHVS45100.45 µm Transparent Styrène Stérile 10
MSDVN65100.65 µm Transparent Styrène Non stérile 10
MSDVN65500.65 µm Transparent Styrène Non stérile 50
MSDVN6B500.65 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 50
MSBVN12101.2 µm Transparent Styrène Non stérile 10
MSBVN12501.2 µm Transparent Styrène Non stérile 50
MSBVS12101.2 µm Transparent Styrène Stérile 10
MSBVN1B501.2 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 50
MVHVN45250.45 µm Opaque Polypropylene Non stérile 25

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Plaques de filtration MultiScreenHTS avec membrane hydrophile en esters de celluloseSupprimer le tri & les filtres Show Filter
RéférenceDimension de poresCode couleurMatériau du dispositifStérilitéConditionnement
MSHAN45100.45 µm Transparent Styrène Non stérile 10
MSHAN45500.45 µm Transparent Styrène Non stérile 50
MSHAS45100.45 µm Transparent Styrène Stérile 10
MSHAN4B500.45 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 50
MSHAS4B100.45 µm Opaque Barex®/TiO<sub>2</sub> Stérile 10

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Plaques de filtration MultiScreenHTS 96 puits avec filtre en fibre de verreSupprimer le tri & les filtres Show Filter
RéférenceDimension de poresCode couleurMatériau du dispositifStérilitéConditionnement
MSFBN6B101.0 / 0.65 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 10
MSFBN6B501.0 / 0.65 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 50
MSFCN6B101.2 / 0.65 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 10
MSFCN6B501.2 / 0.65 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 50
MVFCN12251.2 µm Opaque Polypropylene Non stérile 25

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Plaques de filtration MultiScreenHTS 384 puits avec filtre en fibre de verreSupprimer le tri & les filtres Show Filter
RéférenceDimension de poresCode couleurMatériau du dispositifStérilitéConditionnement
MZFBN0W101.0 / 0.65 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 10
MZFBN0W501.0 / 0.65 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 50
MZFCN0W101.2 / 0.65 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 10
MZFCN0W501.2 / 0.65 µm Opaque Barex®/TiO<sub>2</sub> Non stérile 50

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Plaques de filtration MultiScreen Solvinert avec membrane en PTFE hydrophileSupprimer le tri & les filtres Show Filter
RéférenceDescriptionDimension de poresConditionnement
MSRLN0410MultiScreen Solvinert, PTFE hydrophile, 0,45 µm, transparent, non stérile 0.45 µm 10
MSRLN0450MultiScreen Solvinert, PTFE hydrophile, 0,45 µm, transparent, non stérile 0.45 µm 50
MDRLN0410MultiScreen Solvinert Deep Well, PTFE hydrophile, 0,45 µm, transparent, non stérile 0.45 µm 10

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Plaques de filtration MultiScreen Solvinert avec membrane en PTFE hydrophobeSupprimer le tri & les filtres Show Filter
RéférenceDescriptionDimension de poresConditionnement
MSRPN0410MultiScreen Solvinert, PTFE hydrophobe, 0,45 µm, transparent, non stérile 0.45 µm 10
MSRPN0450MultiScreen Solvinert, PTFE hydrophobe, 0,45 µm, transparent, non stérile 0.45 µm 50
MDRPN0410MultiScreen Solvinert Deep Well, PTFE hydrophobe, 0,45 µm, transparent, non stérile 0.45 µm 10

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AccessoriesSupprimer le tri & les filtres Show Filter
RéférenceDescriptionConditionnement
WP6110060Pompe à vide/pression résistante aux solvants, 100 V/50–60 Hz 1
WP6111560Pompe à vide/pression résistante aux solvants, 115 V/60 Hz 1
WP6122050Pompe à vide/pression résistante aux solvants, 220 V/50 Hz 1
MSCPNPS00Plaque de récupération 96 puits, transparente, non stérile 100
MSCPNPP00Plaque de récupération 96 puits à fond en V 100
MDCPN2M50Plaque de récupération 96 puits Deep Well, polypropylène 50
MACL09600Chargeur de colonnes MultiScreen, 100 µl, noir, aluminium, non stérile 1
MACL09680Chargeur de colonnes MultiScreen, 80 µl, noir, aluminium, non stérile 1
MACL09645Chargeur de colonnes MultiScreen, 45 µl, noir, aluminium, non stérile 1
MACL09625Chargeur de colonnes MultiScreen, 25 µl, noir, aluminium, non stérile 1
MSVMHTSHVMultiScreen High Volume Collar, 1 pk 1

Les chargeurs de colonnes servent à charger les résines de chromatographie pour la séparation sur colonnes.

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Documentation

Références bibliographiques

Aperçu de la référence bibliographiqueApplication
Optimization of a simple, automatable extraction method to recover sufficient DNA from low copy number DNA samples for generation of short tandem repeat profiles.
Schiffner LA, Bajda EJ, Prinz M, Sebestyen J, Shaler R, Caragine TA.
Croat Med J. 2005 Aug;46(4):578-86.  2004

A platform for high-throughput molecular characterization of recombinant monoclonal antibodies
Mark J. Bailey, Andrew D. Hooker, Carolyn S. Adams, Shuhong Zhang, David C. James
J. of Chromatography B, 826 (2005) 177-187, 2005  2004

Antibody Purification

FAQ

QuestionRéponse
The Tech Note "TN053" entitled "Dye Terminator Removal and Sequencing Reaction Clean-up Using Multiscreen 96- Well Filtration Plates" states to add the sequencing reaction to the center of each well (already containing the prepared G-50 resin column). Can I add less than the standard 20ul volumes of sample to each column?Yes. You can add less. When adding volumes less than 10ul you should prewet the resin.
What type of scintillant do you recommend for use in MultiScreen?Although we don't recommend a specific type there are some guidelines. If the reading is done in the MultiScreen plate it important to use a scintilation fluid specific for microtiter plates. The standard scintillation fluid for reading in vials in higher volume and is mixed differently so it shouldn't be used with a microtiter plate.

MicroScint O is for organic and so it would be used with Durapore membrane. In our R&D labs, we have used MicroScint 20 and 40 interchangeably. Supermix (by Wallac) is a microtiter scintillant and has also been used interchangeably with MicroScint 20 and 40.
When I incubate MultiScreen plates, liquid leaks from the bottom of the plate. Is there a way to prevent leakage from the plate?Normally, the Multiscreen filtration plates do not leak when placed on a dry, non absorbent surface during incubation. If you are experiencing leakage, however, be certain that you have asked yourself the following questions:

1. Do I have a high surfactant concentration in the sample?
It is important that you not exceed .1% surfactant in each well.

2. Do I have organic solvents in my solution?
Although the Multiscreen plates have a limited tolerance to organic solevents it is important that you have verified that the concentration and type of solvent used in your assay is compatible with both the membrane and plate plastic. Otherwise, a solvent resistant plate may be more appropriate.

3. Is the underdrain coming in contact with an absorbent surface?
If you lay the plate on lab "chucks" it will cause liquid to wick out from the plate.

4. Have I inspected the plate bottom for microdroplets?
Be sure to blot the plate onto absorbent material prior to all incubations.
What is the well depth and maximum volume capacity of a MultiScreen plate?The well depth of a 96 well MultiScreen plate is 1.245 cm. The well depth for a 384 well MultiScreen plate is 1.2 cm. The maximum working volume of a 96 well plate is 300 ul. The maximum working volume for a 384 well plate is 100 ul.
I am using the Multiscreen system and my samples won't flow through the plate. What's wrong?There are a number of reasons why samples might not flow through the MultiScreen system. Be sure that the vacuum line is clear and not clogged. Be certain that your samples do not have a high particulate load. If they do, you may wish to dilute your samples in filtered buffers. Also, if you are working with cells, make sure that you do not have more than 10^6 cells in each well. If you are over this amount, dilute the cell suspension ot try working with a more open membrane ( larger pore size). Be certain that you do not have the lid on the Multiscreen system. this should be removed prior to filtering in the vacuum manifold. Finally , be sure that if you are not using a whole plate that you have sealed the empty wells with plate sealing tape.

In addition, ensure that the MultiScreen plate is forming a secure seal with the top gasket of the MultiScreen manifold. This can be checked by trying to lift the plate off the manifold with the vacuum on. If the seal is not secure press the four corners of the plate with the vacuum on to seal the plate.
I am using the Multiscreen system and the Multiple Punch assembly for liquid scintillation counting and I am not getting any counts. What might be causing this?Low or inconsistent counts most commonly results from the lack of release of the "labeled" material from the membrane. This is especially an issue with tritium. Millipore has developed a procedure for the release of radioactive label from membrane by adding 0.5 ml of dilute (0.42%) sodium hypochlorite to the scintillation vial containing the "punched" membrane and agitating for thirty minutes prior to the addition of the scintillation cocktail. Please request Technical Brief TB038 for additional details on this procedure.
I can't get any flow through the MultiScreen NA (lysate clearing plate) when performing the plasmid prep protocol. What's wrong?Clogging of the plate is usually due to inappropriate culture medium resulting in overgrowth or lysis. Use 2x LB, not terrific broth or another excessively rich medium.

Produits & Applications associés

Familles de produits

Accessoires pour centrifugation et chromatographie

Combine the cost savings of bulk media with the convenience of 96-well filtration plates.
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Support de filtration sous vide MultiScreenHTS

For vacuum filtration of 96 and 384 samples
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Produits associés classés par : Application Facete

Protein Purification
Sample Preparation
Dialysis and Filtration

Produits associés classés par : Brand Facete

MultiScreen™

Catégories

Life Science Research > Cell Culture and Systems > Cell Culture Flasks, Plates, & Slides > Multiwell Plates