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SCR511 STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit

The STEMCCA Cre-Excisable Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of mouse & human induced pluripotent stem cells.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
SCR511
1 kit  
Purchase on Sigma-Aldrich

Aperçu

Replacement Information
Description
Catalogue NumberSCR511
Trade Name
  • STEMCCA
DescriptionSTEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit
OverviewThe STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Kit contains high titer polycistronic (OKSM) lentivirus and Polybrene transfection reagent that have been validated for the generation of mouse and human induced pluripotent stem cells from mouse embryonic fibroblasts (MEFs) and human foreskin fibroblasts (HFFs), respectively. The Cre-excisable STEMCCA vector, comprised of the transcription factors Oct-4, Klf4, SOX-2, and c-Myc (OKSM) separated by the self-cleaving 2A peptide and IRES sequences, includes flanking LoxP sites that enable Cre-mediated excision of the vector following reprogramming (Sommer CA, 2009). The use of a single polycistronic lentiviral vector significantly improves reprogramming efficiencies and reduces the number of viral integrations. Furthermore, the ability to remove the reprogramming vector has been shown to improve the developmental potential of iPS cells and significantly increase their capacity to undergo directed differentiation in vitro (Sommer CA, 2010).

Important note: the STEMCCA lentivirus reprogramming kits are provided in two formats, dependent on whether you are working with a human or mouse system. Human reprogramming experiments require a higher multiplicity of infection (MOI) than mouse experiments. Therefore, the larger, three-vial kits are required for human reprogramming experiments. If you are reprogramming mouse embryo fibroblast cells, you have the option to use either the one- or three-vial kit, depending on how many wells you want to infect. Reprogramming other rodent somatic cells may require more than one vial of lentivirus to perform at optimal MOI.
References
Product Information
Components
  • 1. EF1α-STEMCCA-LoxP (OKSM) Lentivirus: (Part number CS204439) One (1) vial containing 15 µL of high titer lentivirus.
  • 2. Polybrene Transfection Reagent: (Part number TR-1003-50UL) One (1) vial containing 50 µL of 10 mg/mL stock.
Quality LevelMQ100
Applications
ApplicationThe STEMCCA Cre-Excisable Constitutive Polycistronic Lentivirus Kit contains high titer polycistronic lentivirus & Polybrene transfection reagent that have been validated for the generation of mouse & human induced pluripotent stem cells.
Biological Information
Stem Cell Type
  • Mouse Embryonic Stem Cells
  • Induced Pluripotent Stem Cells
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceTested to confirm the generation of iPS cells from p3 mouse embryonic fibroblasts and p6 human foreskin fibroblasts. Cre-mediated excision of the reprogramming transgenes has only been validated in the mouse system. Other cell types have not been tested and thus similar results can not be guaranteed.
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsLentivirus is stable for at least 6 months when stored at -80°C. After first thaw, place immediately on ice and store in working aliquots to avoid further freeze thaws. Avoid freeze thaws as this will result in a decrease in the virus titer. Polybrene transfection reagent is stable for at least 1 year when stored at -20°C.
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
SCR511 04053252003851

Documentation

STEMCCA Cre-Excisable Constitutive Polycistronic (OKSM) Lentivirus Reprogramming Kit FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Références bibliographiques

Aperçu de la référence bibliographiqueNº PubMed
Excision of viral reprogramming cassettes by Cre protein transduction enables rapid, robust and efficient derivation of transgene-free human induced pluripotent stem cells.
Kadari, Asifiqbal, et al.
Stem Cell Res Ther, 5: 47 (2014)  2014

Afficher le résumé 		24713299 24713299

Brochure

Titre
Simplifying the iPSC Workflow
Stem Cell Research

Fiche technique

Titre
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation
STEMCCA Lentivirus Reprogramming Kits

Posters

Titre
Efficient generation of transgene-free human and mouse iPS cells using a cell-permeant Tat-Cre protein