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14-564M SRPK1 Protein, active, 250 µg

Active, N-terminal GST-tagged recombinant human SRPK1, for use in Kinase Assays.

14-564M
250 µg  Also available in 10 μg size (cat#14-564) and in bulk (cat#14-564-K).
Purchase on Sigma-Aldrich

Aperçu

Replacement Information
Description
Catalogue Number14-564M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionSRPK1 Protein, active, 250 µg
OverviewN-terminal GSTtagged recombinant human SRPK1
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal GST-tagged recombinant human SRPK1, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourceexpressed in E. coli
Entrez Gene Number
Entrez Gene SummaryThis gene encodes a serine/arginine protein kinase specific for the SR (serine/arginine-rich domain) family of splicing factors. The protein localizes to the nucleus and the cytoplasm. It is thought to play a role in regulation of both constitutive and alternative splicing by regulating intracellular localization of splicing factors. A second alternatively spliced transcript variant for this gene has been described, but its full length nature has not been determined.
Gene Symbol
  • SRPK1
  • SFRSK1
Protein TargetSRPK1
Purification MethodGlutathione-agarose
Target Sub-FamilyCMGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q96SB4 # Plays a central role in the regulatory network for splicing, controlling the intranuclear distribution of splicing factors in interphase cells and the reorganization of nuclear speckles during mitosis. Hyperphosphorylates RS domain-containing proteins such as SFRS1 and SFRS2 on serine residues during metaphase but at lower levels during interphase. Locks onto SFRS1 to form a stable complex and processively phosphorylates the RS domain. Appears to mediate HBV core protein phosphorylation which is a prerequisite for pregenomic RNA encapsidation into viral capsids.
COFACTOR: Magnesium.
SIZE: 826 amino acids; 92407 Da
SUBUNIT: Present in a seven component complex, the toposome, which separates entangled circular chromatin DNA during chromosome segregation. The extended N-terminal domain of isoform 1 binds to the nuclear scaffold-associated protein SAFB suggesting this isoform may phosphorylate splicing factors in close vicinity to the nuclear matrix.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Isoform 1 and isoform 2 are cytoplasmic and nuclear.TISSUE SPECIFICITY: Isoform 2 is predominantly expressed in the testis but is also present at lower levels in heart, ovary, small intestine, liver, kidney, pancreas and skeletal muscle. Isoform 1 is only seen in the testis, at lower levels than isoform 2.
SIMILARITY: SwissProt: Q96SB4 ## Belongs to the protein kinase superfamily. Ser/Thr protein kinase family. & Contains 1 protein kinase domain.
Molecular Weight100kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of SRPK1tide
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-564) and in bulk (cat#14-564-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
14-564M 04053252924163

Documentation

Produits & Applications associés

Alternative Packsize

Référence Description
14-564 SRPK1 Protein, active, 10 µg

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Catégories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases