Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

567693 SOST, Mouse, Recombinant

SOST, Mouse, Recombinant : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
567693
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Tableau de caractéristiques principal

Purity
≥90% by SDS-PAGE

Products

RéférenceConditionnement Qté
567693-25UG Flacon en verre 25 μg
Description
OverviewRecombinant, mouse sclerostin (SOST) consisting of amino acids 24-211, expressed in NS0-derived murine myeloma cells with an N-terminal His•Tag®. SOST is expressed by osteoclasts in developing bones of mouse embryos and acts as a negative regulator of bone formation through repression of BMP-induced osteogenesis.
Catalogue Number567693
Brand Family Calbiochem®
References
ReferencesKusu, N. et al. 2003. J. Biol. Chem. 278, 24113.
Balemans, W. et al. 2001. Hum. Mol. Genet. 10 537.
Brunkow, M.E. et al. 2001. Am. J. Hum. Genet. 68, 577.
Product Information
FormSolid
FormulationLyophilized from 0.2 µm filter-sterilized PBS, BSA.
Quality LevelMQ100
Applications
Biological Information
Biological activityInhibition Assay: Inhibits hBMP-6-induced alkaline phosphatase by 50-80% in MC3T3-E1 mouse osteoblastic cells when used at a concentration of 30 µg/ml.
Purity≥90% by SDS-PAGE
Physicochemical Information
ContaminantsEndotoxin: <1.0 EU/µg protein
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
567693-25UG 04055977190779

Documentation

SOST, Mouse, Recombinant FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

SOST, Mouse, Recombinant Certificats d'analyse

TitreNuméro de lot
567693

Références bibliographiques

Aperçu de la référence bibliographique
Kusu, N. et al. 2003. J. Biol. Chem. 278, 24113.
Balemans, W. et al. 2001. Hum. Mol. Genet. 10 537.
Brunkow, M.E. et al. 2001. Am. J. Hum. Genet. 68, 577.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision21-Sepmtember-2011 RFH
DescriptionRecombinant, mouse sclerostin (SOST) consisting of amino acids 24-211, expressed in NS0-derived murine myeloma cells with an N-terminal His•Tag®. SOST is expressed by osteoclasts in developing bones of mouse embryos and acts as a negative regulator of bone formation through repression of BMP-induced osteogenesis.
FormSolid
FormulationLyophilized from 0.2 µm filter-sterilized PBS, BSA.
Purity≥90% by SDS-PAGE
ContaminantsEndotoxin: <1.0 EU/µg protein
Biological activityInhibition Assay: Inhibits hBMP-6-induced alkaline phosphatase by 50-80% in MC3T3-E1 mouse osteoblastic cells when used at a concentration of 30 µg/ml.
SolubilityReconstitute to 200 µg/ml in sterile PBS containing at least 0.1% human or bovine serum albumin.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesKusu, N. et al. 2003. J. Biol. Chem. 278, 24113.
Balemans, W. et al. 2001. Hum. Mol. Genet. 10 537.
Brunkow, M.E. et al. 2001. Am. J. Hum. Genet. 68, 577.