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03-0199-00 SMC™ Human NPTX2 High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human NPTX2 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of NPTX2 in human serum and plasma.

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Key Applications: Single Molecule Counting (SMC®) Research Category: Neuroscience, neurological Research Sub-Category: Alzheimer's Disease, Neurodegenerative Diseases, Neuroscience
03-0199-00
96 well assay  
Purchase on Sigma-Aldrich

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Tableau de caractéristiques principal

Key Applications
Single Molecule Counting (SMC®)
Description
Catalogue Number03-0199-00
Trade Name
  • SMC®
DescriptionSMC™ Human NPTX2 High Sensitivity Immunoassay Kit
OverviewHuman NPTX2 Immunoassay Kit for the Quantitative Determination of NPTX2 in Human Serum, Plasmaand Cerebrospinal Fluid.
Background InformationPentraxins are a superfamily of multimeric calcium-dependent binding proteins, and can be classified into short and long subfamilies. A member of the latter group, Human Neuronal Pentraxin 2 (NPTX2) is known to accumulate at excitatory synapses of neurons, and play roles in guiding synaptic plasticity in healthy central nervous systems. Meanwhile, results from recent studies have indicated the potential role of NPTX2 in neurodegenerative disorders, as decreases in this protein has been associated with diseases such as Alzheimer’s disease, suggesting the value of studying this protein in low abundance. The SMC™ Human NPTX2 High Sensitivity Immunoassay Kit harnesses the power of digital Single Molecule Counting technology to enable researchers to better understand the dynamics of this protein in normal and aberrant neurology, by achieving accurate and reproducible measurements of NPTX2 concentrations in human cerebrospinal fluid (CSF) and blood samples.
References
Product Information
Components
  • Assay Buffer
  • NPTX2 Coated Beads
  • Standard Diluent
  • NPTX2 Detection Antibody
  • NPTX2 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
Precision, %
  • The assay variations of SMC™ Human NPTX2 Immunoassay kit were studied
    using five normal plasma samples run in triplicate by 3 different operators on 3 different days.
  • Mean intra-assay variation was 6%.
  • Mean inter-assay variation was 5%.
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human NPTX2 High Sensitivity Immunoassay Kit unparalleled performance in the quantification of NPTX2 in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has the following features:
• Improved options for sample prep and incubation
• Lyophilization protein standard for uniform shipping and storage condition
• Increased reagent volumes to accommodate automation
• Able to achieve assay specifications on both SMCxPRO™ and Erenna®.
Biological Information
Species Reactivity NoteSpecificity to the following species samples were tested with the follow results:
Non human primate – 100%
Mouse – 100%
Rat – 100%
Canine – 100%
Feline – 100%
Cross ReactivityN/A
Accuracy
  • Minimum of 5 serum samples spiked with average recovery of 99% with a range of 93-103%
  • Minimum of 5 plasma samples spiked with average recovery of 104% with a range of 97-118%
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.58 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
03-0199-00 04065266416428