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03-0155-00 SMC™ Human Interleukin 6 (IL-6) High Sensitivity Immunoassay Kit

The sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human Interleukin 6 (IL-6) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-6 in human serum and plasma.

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Key Applications: Single Molecule Counting (SMC®) Research Category: Inflammation & Immunology Research Sub-Category: Inflammatory Disease, Immune Response, Autoimmune disease
03-0155-00
96 well assay  
Purchase on Sigma-Aldrich

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Replacement Information

Tableau de caractéristiques principal

Species ReactivityKey Applications
NhPSingle Molecule Counting (SMC®)
Description
Catalogue Number03-0155-00
Trade Name
  • SMC®
DescriptionSMC™ Human Interleukin 6 (IL-6) High Sensitivity Immunoassay Kit
OverviewThe SMC™ Human IL-6 High Sensitivity Immunoassay Kit contains all reagents required to perform the assay. This assay takes advantage of a standard immunoassay workflow configured in a standard plate format. Assay protocols are similar to existing sandwich ELISA methods with two key differences: 1) Elution buffer disrupts the sandwich, separating the labeled detection antibody for quantification. 2) The Erenna® and SMCxPRO™ immunoassay systems detect analytes using Single Molecule Counting (SMC™) technology.
Background InformationInterleukin 6 (IL-6) is secreted by T cells and macrophages and acts as both a pro-inflammatory cytokine and an anti-inflammatory myokine. Some of its functions include the stimulation of the immune response during infection, induction of fever in autoimmune, infectious and non-infectious diseases, the stimulation of acute phase reactions, differentiation of T cells, proliferation and differentiation of B-cells, the development of blood cells as well as the activation of osteoclasts and osteoporosis and the secretion of vascular endothelium growth factor (VEFG) to increase blood vessel growth and vascular permeability in inflammation. It is important for a short-term defense against infection, injury and inflammation however dysregulation of IL-6 results in disease.

IL-6 is important in the study of diseases such as diabetes, lupus, multiple myeloma, rheumatoid arthritis and some cancers such as prostate and pancreatic cancer. Conversely, deficiency of IL-6 can have an effect on immune activation and IgA antibodies while overexpression of IL-6 can result in an immunological imbalance leading to an autoimmune pathology.
References
Product Information
Components
  • Assay Buffer
  • Human IL-6 Beads
  • Standard Diluent
  • Human IL-6 Detection Antibody
  • Human IL-6 Standard
  • 10X Wash Buffer
  • Buffer D
  • Elution Buffer B
HS Code3002 15 90
Precision, %
  • Intra-assay CV% average (n=20): <15%
  • Inter-assay CV% average (n=6): <20%
Applications
ApplicationThe sensitivity of the Single Molecule Counting (SMC™) technology gives the SMC™ Human Interleukin 6 (IL-6) High Sensitivity Immunoassay Kit unparalleled performance in the quantification of IL-6 in human serum and plasma.
Key Applications
  • Single Molecule Counting (SMC®)
Application NotesThis new streamline kit has been improved with the following features while the kit performance is consistent with the legacy IL-6 kit, #03-0089-01:
• Improved options for sample prep and incubation
• Lyophilization of the standard provides the kit at one storage condition
• Overnight incubation can be used
• Increased reagent volumes to accommodate automation
• Validated to run on both SMCxPRO™ and Erenna®
Biological Information
Species Reactivity
  • Non-human Primate
Species Reactivity NoteAlthough IL-6 was detectable in non-human primate (rhesus macaque and cynomolgus monkey) samples, the assay has not been validated for these species.
Accuracy
  • Minimum of 5 samples spiked with average recovery of 107% with a range of 90-114%.
Physicochemical Information
Sensitivity
  • Lower Limit of Quantification: 0.08 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size96 well assay
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
03-0155-00 04054839520655