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HTS211RTA Ready-to-Assay™ P2Y4 Purinergic Receptor Frozen Cells

Human P2Y4 GPCR frozen cells for Calcium Flux FLIPR Assays.

Host Cell: 1321N1 Entrez Gene Number: NM_002565.3
HTS211RTA
1 kit  
Purchase on Sigma-Aldrich

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Tableau de caractéristiques principal

SpeciesHost CellsProtein Target
Human 1321N1P2Y4
Description
Catalogue NumberHTS211RTA
Trade Name
  • Ready-to-Assay
DescriptionReady-to-Assay™ P2Y4 Purinergic Receptor Frozen Cells
Background InformationMillipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium response.

The P2Y GPCRs serve as receptors for extracellular nucleotides. The P2Y4 receptor is activated by UTP, and also by ATP in rodents, and couples to Gq to increase intracellular calcium (von Kügelgen, 2006). P2Y4 is expressed prominently in the intestinal epithelium, where it regulates intestinal chloride secretion. P2Y4 therefore is likely to be involved in infectious diarrhea caused by nucleotides released upon infection with enteropathogenic bacteria (Robaye et al., 2003). Millipore’s cloned human P2Y4-expressing cell line is made in the 1321N1host, which supports high levels of recombinant P2Y4 expression on the cell surface. Thus, the cell line is an ideal tool for screening for agonists and antagonists of P2Y4.
References
Product Information
Components
  • Pack contains 2 vials of mycoplasma-free cells, 1 ml per vial.
  • Fifty (50) mL of Media Component.
Detection methodFluorescent
Quality LevelMQ300
Applications
ApplicationHuman P2Y4 GPCR frozen cells for Calcium Flux FLIPR Assays.
Key Applications
  • Calcium Flux Assays
Biological Information
Entrez Gene Number
GPCR ClassA
Host Cells1321N1
Protein TargetP2Y4
Target Sub-FamilyP2Y / Purinergic (metabotropic)
Cell Line Type
  • GPCR Cell Lines
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • This product contains genetically modified organisms (GMO). Within the EU GMOs are regulated by Directives 2001/18/EC and 2009/41/EC of the European Parliament and of the Council and their national implementation in the member States respectively. This legislation obliges Merck to request certain information about you and the establishment where the GMOs are being handled. Click here for Enduser Declaration (EUD) Form.
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Packaging Information
Material Size1 kit
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
HTS211RTA 04053252555312

Documentation

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Ready-to-Assay™

Catégories

Life Science Research > Cell Culture and Systems > Cell Lines > GPCR Frozen Cells
Life Science Research > Drug Discovery and Development > GPCR Cell Lines & Assays > GPCR Frozen Cells