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553506 RAP, Human, Recombinant, E. coli

RAP, Human, Recombinant, E. coli : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: Receptor Associated Protein

553506
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
553506-50UG Ampoule plast. 50 μg
Description
OverviewRecombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low density lipoprotein receptor-related protein (LRP) and very low-density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low density lipoprotein receptor (LDLR) and several of its class 2 mutants. Expressed as a GST-fusion protein that is subsequently cleaved with thrombin. Typical concentrations to block LRP or VLDL receptor are 200-300 nM. For antagonism of LDLR, 1 µM is recommended.
Catalogue Number553506
Brand Family Calbiochem®
SynonymsReceptor Associated Protein
References
ReferencesLi, Y., et al. 2002. Biochemistry 41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
Bu, G. 2001. Int. Rev. Cytol. 209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035
Product Information
FormLiquid
Formulation150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
553506-50UG 04055977193664

Documentation

RAP, Human, Recombinant, E. coli FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

RAP, Human, Recombinant, E. coli Certificats d'analyse

TitreNuméro de lot
553506

Références bibliographiques

Aperçu de la référence bibliographique
Li, Y., et al. 2002. Biochemistry 41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
Bu, G. 2001. Int. Rev. Cytol. 209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision20-April-2009 JSW
SynonymsReceptor Associated Protein
DescriptionRecombinant, human RAP fused to GST, expressed in E. coli, and subsequently cleaved with thrombin to remove GST. Receptor Associated Protein (RAP) antagonizes the ligand binding of certain members of the low-density lipoprotein receptor family such as low-density lipoprotein receptor-related protein (LRP) and very low density lipoprotein (VLDL) receptor. Promotes the proper folding and subsequent exocytic trafficking of the wild-type low-density lipoprotein receptor (LDLR) and several of its class 2 mutants. Typical concentrations used to block LRP or VLDL receptor are 200-300 nM; for antagonism of LDLR, 1 µM is recommended.
FormLiquid
Formulation150 mM NaCl, 100 mM Tris-HCl, pH 7.4.
Concentration Label Please refer to vial label for lot-specific concentration
Purity≥90% by SDS-PAGE
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesLi, Y., et al. 2002. Biochemistry 41, 4921.
Salicioni, A.M., et al. 2002. J. Biol. Chem. 277, 16160.
Anderson, O.M., et al. 2001. Biochemistry 40, 15408.
Bu, G. 2001. Int. Rev. Cytol. 209, 79.
Williams, S.E., et al. 1992. J. Biol. Chem. 267, 9035