Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

IP04 Protein G Plus-Agarose Suspension

Protein G PLUS agarose suspension specifically formulated for immunoprecipitation.

Protein G Plus-Agarose Suspension : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
IP04
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
IP04-1.5ML Ampoule plast. 1.5 ml
Description
OverviewDesigned for immunoprecipitation applications. This product is blocked with BSA to reduce non-specific binding and cannot be used for purification; best for bovine, goat, human, and rat IgG and for mouse IgG1.
Catalogue NumberIP04
Brand Family Calbiochem®
References
Product Information
FormLiquid slurry
FormulationIn PBS.
Preservative≤0.1% sodium azide
Applications
Key Applications Immunoprecipitation
Application NotesImmunoprecipitation (see comments)
Application CommentsAgarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
IP04-1.5ML 07790788053833

Documentation

Protein G Plus-Agarose Suspension FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Protein G Plus-Agarose Suspension Certificats d'analyse

TitreNuméro de lot
IP04
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision04-September-2007 RFH
ApplicationImmunoprecipitation (see comments)
DescriptionProtein G PLUS agarose suspension specifically formulated for immunoprecipitation.
BackgroundProtein G PLUS-Agarose has multiple Ig binding sites genetically engineered for higher binding capacity of mouse IgG1 or IgG3 as well as IgG from rabbit, rat, and other species. Mouse IgG2a or IgG2b bind moderately to Protein G PLUS. As an alternative, use Protein A, Protein G PLUS/Protein A, or goat second-step antibodies to bridge these antibodies to Protein G PLUS. For rat IgM antibodies, use goat second-step antibodies with Protein G PLUS or Protein G PLUS/Protein A.
FormLiquid slurry
FormulationIn PBS.
Preservative≤0.1% sodium azide
CommentsAgarose solution is supplied ready to use. Protein G Plus agrarose is blocked with BSA and should not be used for immunoglobulin purification or covalent cross-linking. For immunoprecipitation reactions 15 µl/µg primary antibody is recommended. Preclearing will minimize extra bands resulting from nonspecific precipitation. To preclear, add to the sample 20 µl agarose conjugate and 1 µg normal IgG from the same species as the immunoprecipitating antibody. When immunoblotting is used for detection, some secondary antibodies can react nonspecifically with BSA or other proteins present at high concentrations in the sample. This can be eliminated by reducing the concentration of secondary antibody.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling

Produits & Applications associés

Related Products

Référence Description
IP02 Protein A Agarose Suspension
IP05 Protein G Plus/Protein A Agarose Suspension
IP10 Protein G Plus/Protein A-Agarose

Catégories

Life Science Research > Protein Sample Preparation > Protein Purification > Agarose Bead Affinity Purification > Agarose Beads for IP & Antibody Purification
Life Science Research > Protein Detection and Quantification > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification
Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Agarose Beads for IP & Antibody Purification