Millipore Sigma Vibrant Logo
Attention: We have moved. Merck Millipore products are no longer available for purchase on MerckMillipore.com.Learn More

69049 Pellet Paint® Co-Precipitant

A fluorescent dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (DNA and RNA pellets)

Pellet Paint® Co-Precipitant : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
69049
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
69049-3 Ampoule plast. 125 rxn
69049-4 Ampoule plast. 1000 rxn
Description
OverviewPellet Paint® Co-Precipitant is a visible dye-labeled carrier formulated specifically for use in alcohol precipitation of nucleic acids (McCormick 1995, McCormick 1996). The two-minute precipitation uses just 2 µl per reaction and requires no low-temperature incubations or prolonged centrifugation. Both RNA and DNA are efficiently precipitated from even dilute solutions (2 ng/ml) and the pellet is easily located by its vivid pink color. The pellet can be easily followed during washing steps and prevents losses during handling.

Most applications of PCR benefit from a clean-up step in which primers and other reactants are removed and the target DNA is concentrated (Taggart 1998). Pellet Paint Co- Precipitant is ideal for this cleanup because the procedure is rapid, primers < 50 nt in length are efficiently removed, and the DNA is quantitatively recovered. Furthermore, it is easy to tell when the DNA has been completely resuspended following the precipitation step.

Pellet Paint Co-Precipitant is compatible with most molecular biology procedures and is free of contaminating nucleic acids and nucleolytic enzymes. Although it absorbs in the UV range, accurate spectrophotometric measurements of DNA or RNA samples are possible; the absorbance ratio (provided with each package) can be used as a correction factor when determining nucleic acid concentration (McCormick 1996). Pellet Paint Co-Precipitant is compatible with automatted Cy5® sequencers. Pellet Paint NF Co- Precipitant is recommended for use with PE Applied Biosystems automated sequencers.
Catalogue Number69049
Brand Family Novagen®
Features and benefits• Direct visualization and tracking of precipitated material
References
ReferencesMcCormick, M. 1995. inNovations 4a, 10. McCormick, M. 1996. inNovations 5, 10. Taggart, E. W., et al. 1998. J. Clin. Microbiol. 36, 3408.
Product Information
•250 µl or 2 mlPellet Paint Co-Precipitant
•1 ml or 8 ml3 M Sodium Acetate, pH 5.2
DeclarationThis product is covered by U.S. Patents 7,550,447, 7,144,713, EP Patent 853,680, Canadian Patent 2,231,501 owned by EMD Chemicals Inc. or its Affiliates
FormLiquid
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
69049-3 04055977259797
69049-4 04055977259803

Documentation

Pellet Paint® Co-Precipitant FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Pellet Paint® Co-Precipitant Certificats d'analyse

TitreNuméro de lot
69049

Références bibliographiques

Aperçu de la référence bibliographique
McCormick, M. 1995. inNovations 4a, 10. McCormick, M. 1996. inNovations 5, 10. Taggart, E. W., et al. 1998. J. Clin. Microbiol. 36, 3408.

Brochure

Titre
High fidelity gene amplification

Citations

Titre
  • Brooks, C.L., 2007. Journal of Biological Chemistry 282, 22804.
  • Buttner, M.P., et al. 2007. Applied and Enviornmental Microbiology 73, 3505.
  • Halling, R.E., et al. 2007. Mycologia 99, 310.
  • Quan, S., et al. 2007. Journal of Biological Chemistry 282, 28823.
  • Tsai, C.J., et al. 2007. Procedings of the National Academy of Science 104, 7851.
  • Nowak, J.E., et al. 2006. Bio/Techniques 40, 220.
  • Lee, Y., et al. 2005 Clinical Cancer Research 11, 1059.
  • Mori, T., et al. 2005. Journal of Clinical Oncology 23, 9351.
  • Pendleton, L.C., et al. 2005. Journal of Biological Chemistry 280, 24252.
  • Beischlag, T.V., et al. 2004. Journal of Biological Chemistry 279, 54620.
  • Xu, M., et al. 2002. Journal of Clinical Microbiology 40, 2626.
  • Taggart, E.W., et al. 1998. Journal of Clinical Microbiology 36, 3408.
    		•

    Protocoles Utilisateur

    Titre
    TB146 Pellet Paint® Co-Precipitant