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14-440 PKA Protein, Recombinant, 10 µg

Recombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.

PKA Protein, Recombinant, 10 µg : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
14-440
10 µg  Also available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.
Purchase on Sigma-Aldrich

Aperçu

Replacement Information
Description
Catalogue Number14-440
Replaces14-114
Brand Family Upstate
Trade Name
  • Upstate
DescriptionPKA Protein, Recombinant, 10 µg
OverviewRecombinant human full length PKA, catalytic subunit type alpha, untagged
References
Product Information
HS Code3507 90 19
Quality LevelMQ100
Applications
ApplicationRecombinant human full length PKA, catalytic subunit type alpha, untagged, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
SourceExpressed in E.coli BL21 (DE3) pLysS cells
Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Entrez Gene Number
Entrez Gene SummarycAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the cAMP-dependent protein kinase, which transduces the signal through phosphorylation of different target proteins. The inactive kinase holoenzyme is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits have been identified in humans. The protein encoded by this gene is a member of the Ser/Thr protein kinase family and is a catalytic subunit of cAMP-dependent protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Gene Symbol
  • PRKACA
  • MGC102831
  • PKACA
  • MGC48865
Protein TargetPKA
Purification MethodPhosphocellulose P11 chromatography
Target Sub-FamilyAGC
UniProt Number
UniProt SummaryFUNCTION: SwissProt: P17612 # Phosphorylates a large number of substrates in the cytoplasm and the nucleus.
SIZE: 351 amino acids; 40590 Da
SUBUNIT: A number of inactive tetrameric holoenzymes are produced by the combination of homo- or heterodimers of the different regulatory subunits associated with two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits.
SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Translocates into the nucleus (monomeric catalytic subunit) (By similarity). The inactive holoenzyme is found in the cytoplasm (By similarity).
TISSUE SPECIFICITY: Isoform 2 is sperm specific.
PTM: Asn-3 is partially deaminated to Asp giving rise to 2 major isoelectric variants, called CB and CA respectively (By similarity).
SIMILARITY: SwissProt: P17612 ## Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. cAMP subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 protein kinase domain.
Molecular Weight40.7kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality Assuranceroutinely evaluated by phosphorylation of Kemptide substrate in vitro
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions1 year at -70°C
Packaging Information
Material Size10 µg
Material PackageAlso available in 250 µg size --call for pricing and availability and reference catalog number 14-440M when ordering the 250 µg size.
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
14-440 04053252588150

Documentation

PKA Protein, Recombinant, 10 µg FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

PKA Protein, Recombinant, 10 µg Certificats d'analyse

TitreNuméro de lot
PKA, catalytic subunit, recombinant - D8MN079U-I D8MN079U-I
PKA, catalytic subunit, recombinant - D8MN079U-J D8MN079U-J
PKA, catalytic subunit, recombinant - D8MN079U-K D8MN079U-K
PKA, catalytic subunit, recombinant - D8MN079U-M D8MN079U-M

Références bibliographiques

Aperçu de la référence bibliographiqueApplicationNº PubMed
Cell surface expression of the ROMK (Kir 1.1) channel is regulated by the aldosterone-induced kinase, SGK-1, and protein kinase A
Yoo, D., et al
J Biol Chem, 278:23066-75 (2003)  2003

Kinase Assay12684516 12684516
Prokaryotic expression of catalytic subunit of adenosine cyclic monophosphate-dependent protein kinase.
Yonemoto, W M, et al.
Meth. Enzymol., 200: 581-96 (1991)  1991

1956337 1956337

Produits & Applications associés

Alternative Packsize

Référence Description
14-440M PKA Protein, Recombinant, 250 µg

Familles de produits

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Catégories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases