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S7151 OxyBlot™ Protein Standard

OxyBlot Protein Standard serves as an internal control for the electrophoresis, western transfer & immunodetection steps of the OxyBlot procedure.

OxyBlot™ Protein Standard : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Key Applications: Western Blotting
S7151
150 µL  
Purchase on Sigma-Aldrich

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Tableau de caractéristiques principal

Key Applications
WB
Description
Catalogue NumberS7151
Brand Family Chemicon®
Trade Name
  • OxyBlot
  • Chemicon
DescriptionOxyBlot™ Protein Standard
OverviewOxyBlot™ Protein Standard is composed of five proteins (phosphorylase B, BSA, ovalbumin, carbonic anhydrase, trypsin inhibitor) containing 1-3 dinitrophenylhydrazone (DNP) residues per protein. These attached DNP moieties permit the visualization of the proteins by the detection system of the OxyBlot™ Protein Oxidation Detection Kit.

Intended Use: OxyBlot™ Protein Standard serves as an internal control for the electrophoresis, western transfer and immunodetection steps of the OxyBlot™ procedure.
References
Product Information
Components
  • Each vial contains 150 μl of OxyBlot Protein Standard, which is sufficient for 60 minigels.
HS Code3822 19 90
Quality LevelMQ100
Applications
ApplicationOxyBlot Protein Standard serves as an internal control for the electrophoresis, western transfer & immunodetection steps of the OxyBlot procedure.
Key Applications
  • Western Blotting
Application NotesMaterial Provided:

Each vial contains 150 μl of OxyBlot™ Protein Standard, which is sufficient for 60 minigels.

Instructions for Use:

Warm the mixture to room temperature. (Since the mixture contains SDS that may precipitate during storage, it may be necessary to heat the stock solution to 50°C to redissolve the SDS). Combine 2.5 μl of the OxyBlot™ Protein Standard with 20 μl of 1X Gel Loading Buffer prior to loading onto the minigel. Do not heat prior to gel loading. Perform SDS-PAGE according to standard procedures.



NOTE: Since the proteins in the standard mixture already contain DNP residues, the protein standard should not be subjected to the derivatization reaction in the OxyBlot™ Protein Oxidation Detection Kit protocol.



In order to maintain consistency, the 2-Mercaptoethanol must be omitted from the 1X Gel Loading Buffer if a reducing agent was not used in the preparation of the experimental samples.



Reagent Preparation:

1X Gel Loading Buffer

To make 8 ml:

0.5 M Tris-HCl, pH 6.8 1.0 ml

Glycerol 0.8 ml

14.3 M 2-Mercaptoethanol (Reagent Grade) 0.1 ml

10% SDS 1.6 ml

0.05% Bromophenol Blue 0.32 ml

dH2O 4.18 ml

Divide into 1 ml aliquots and store at -15°C to -25°C.



Protein Molecular Weight

Phosphorylase B 97,400

Bovine serum albumin 68,000

Ovalbumin 43,000

Carbonic anhydrase 29,000

Trypsin inhibitor 21,000



OxyBlot™ is a trademark of Serologicals Company
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsStore at -25° to -15°C upon receipt. After using for the first time, divide the remaining mixture among several tubes. Dividing the mixture into aliquots serves to protect the mixture from excessive freeze-thaw cycles.
Packaging Information
Material Size150 µL
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
S7151 04053252504051

Documentation

OxyBlot™ Protein Standard FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

OxyBlot™ Protein Standard Certificats d'analyse

TitreNuméro de lot
OxyBlot™ Protein Standard 3158878
OxyBlot™ Protein Standard 3012112
OxyBlot™ Protein Standard 2905363
OxyBlot™ Protein Standard 2939625
OxyBlot™ Protein Standard 3111167
OxyBlot™ Protein Standard 3050673
OxyBlot™ Protein Standard - 3188635 3188635
OxyBlot™ Protein Standard - 3454407 3454407
OxyBlot™ Protein Standard - 3546903 3546903
OxyBlot™ Protein Standard - 3738457 3738457
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