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72621 NovaQUANT™ Mouse Mitochondrial to Nuclear Ratio kit

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72621
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Products

RéférenceConditionnement Qté
72621-1KIT Flacon en verre 1 kit
Description
OverviewProduct Description
The NovaQUANT™ Mouse Mitochondrial to Nuclear DNA Ratio Kit compares the levels of nuclear to mitochondrial DNA (mtDNA) in a mouse DNA sample. Real-Time PCR provides a platform for a quantitative assay measuring and comparing mtDNA copy number to that of nuclear DNA (nDNA). This mtDNA/nDNA ratio test kit in qPCR format can be used whenever a small amount of DNA can be extracted from cells or tissues of interest. The amplification primers in this kit have been thoroughly validated for matching amplification efficiency and absence of amplification of mitochondrial pseudogenes.

This kit contains a set of four optimized PCR primer pairs targeting two nuclear and two mitochondrial genes. A single assay is run using four wells, whereby each well individually tests for one of four gene targets (see Figure 1 at the bottom of this page). Resultant Cts obtained from the Real-Time instrument are used to represent the level of each gene. Nuclear gene 1 is compared to that of mitochondrial gene 1 and nuclear gene 2 to mitochondrial gene 2. In this way, ratios are calculated which can be averaged to represent the mtDNA copy number per cell.

Panel Components
• 2 ea           Mouse qPCR plate with pre-aliquoted PCR primer pairs
• 100 ng       Mouse total DNA (10 ng/µl)
• 100 ng       Mouse rho zero DNA (10 ng/µl)

Storage: Store all components at 4 °C for up to three months; we recommend storing the control DNA at –20 °C for long term storage.
Catalogue Number72621
Brand Family Novagen®
Application Data
References
Product Information
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
72621-1KIT 04055977266108