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70666 Ni-NTA His•Bind® Resin

70666
Purchase on Sigma-Aldrich

Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
70666-3 Ampoule plast. 10 ml
70666-4 Flacon en verre 25 ml
70666-5 Flacon en verre 100 ml
70666-6 Flacon en verre 500 ml
Description
OverviewNi-NTA His•Bind® Resin is a high-performance Ni2+-charged agarose used for rapid one-step purification of proteins containing a His•Tag® sequence by metal chelation chromatography. NTA chemistry minimizes metal leaching during purification and is compatible with up to 10 mM β-mercaptoethanol or 1 mM Tris(hydroxypropyl)phosphine (THP) for reduction of disulfide bonds. Ni-NTA His•Bind® Resin has a binding capacity of 5–10 mg His•Tag® fusion protein per ml resin.

Supplied as a 50% slurry; the quantity/pack is based on the amount of the settled resin.
Catalogue Number70666
Brand Family Novagen®
References
Product Information
Quality LevelMQ400
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 10-22-42/43

Flammable.
Harmful if swallowed.
May cause sensitization by inhalation and skin contact.
S PhraseS: 26-36-7

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Keep container tightly closed.
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped at Ambient Temperature or with Blue Ice
Toxicity Flammable
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
70666-3 07790788054946
70666-4 07790788052768
70666-5 04055977334074
70666-6 04055977256826

Documentation

Ni-NTA His•Bind® Resin FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Ni-NTA His•Bind® Resin Certificats d'analyse

TitreNuméro de lot
70666

Brochure

Titre
Protein Purification and Detection Tools

Informations techniques

Titre
Ni-NTA HisユBind® Resins

Citations

Titre
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  • D. Andrew Skaff, et al. (2005) Glucose 6-phosphate release of wild-type and mutant human brain hexokinases from mitochondria. Journal of Biological Chemistry 280, 38403-38409.
  • James A. Snipes, et al. (2005) Cloning and characterization of cyclooxygenase-1b (putative cyclooxygenase-3) in rat. Journal of Pharmacology and Experimental Therapeutics 313, 668-676.
  • Taishi Tsubouchi, et al. (2005) Leader peptide-mediated transcriptional attenuation of lysine biosynthetic gene cluster in Thermus thermophilus. Journal of Biological Chemistry 280, 18511-18516.
  • Matthew W. Vetting, et al. (2005) A novel dimeric structure of the rimL N-acetyltransferase from Salmonella typhimurium. Journal of Biological Chemistry 280, 22108-22114.
  • Aileen Aherne, et al. (2004) On the molecular patholo
  • Protocoles Utilisateur

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    TB273 Ni-NTA His•Bind® Resin