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480012 NDSB Set - Calbiochem

Contains the following non-detergent sulfobetaines: 5 g of NDSB-195, 25 g of NDSB-201, and 5 g of NDSB-256.

NDSB Set - Calbiochem : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
480012
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Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
480012-1SET Flacon en verre 1 set
Description
OverviewContains the following non-detergent sulfobetaines: 5 g of NDSB-195 (Cat. No. 480001), 25 g of NDSB-201 (Cat. No. 480005), and 5 g of NDSB-256 (Cat. No. 480010). Supplied with a data sheet.
Catalogue Number480012
Brand Family Calbiochem®
References
ReferencesGoldberg, M.E., et al. 1996. Folding and Design 1, 21.
Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
Vuillard, L., et al. 1995. Biochem. J. 305, 337.
Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
Vuillard, L., et al. 1994. FEBS Lett. 353, 294.
Product Information
FormSolid
Hygroscopic Hygroscopic
Quality LevelMQ100
Applications
Biological Information
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
R PhraseR: 36/37/38

Irritating to eyes, respiratory system and skin.
S PhraseS: 26-36

In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Irritant
Storage +15°C to +30°C
Hygroscopic Hygroscopic
Do not freeze Ok to freeze
Special InstructionsStore lyophilized products at room temperature. Following reconstitution, store in the refrigerator (4°C) or at room temperature (20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
480012-1SET 04055977201819

Documentation

NDSB Set - Calbiochem FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

NDSB Set - Calbiochem Certificats d'analyse

TitreNuméro de lot
480012

Références bibliographiques

Aperçu de la référence bibliographique
Goldberg, M.E., et al. 1996. Folding and Design 1, 21.
Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
Vuillard, L., et al. 1995. Biochem. J. 305, 337.
Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
Vuillard, L., et al. 1994. FEBS Lett. 353, 294.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision11-August-2008 RFH
DescriptionNon-detergent sulfobetaines (NDSBs) are zwitterionic compounds. Like zwittergents (zwitterionic detergents), NDSBs carry the sulfobetaine hydrophilic head group. However, unlike zwittergents, the hydrophobic group in NDSBs is too short for micellar formation even at concentrations as high as 1M. Hence, NDSBs do not behave like detergents. Because of their zwitterionic nature, NDSBs were first employed to neutralize electrostatic interactions without increasing the conductivity in native isoelectrofocusing gels. Recently they have found utility in various other applications including isolation of membrane proteins, purification of nuclear proteins and halophilic proteins, renaturation of chemically and thermally denatured proteins and protein crystallization. They are potentially useful in solubilization and renaturation of fusion proteins from inclusion bodies. It is hypothesized that the hydrophobic group, although short, interacts with the hydrophobic regions of the protein preventing aggregation during renaturation. Also, presumably, the contribution from the short hydrophobic groups combined with charge neutralization ability of the sulfobetaine group results in higher yields of membrane proteins.

NDSBs do not interfere with enzymatic assays based on chromogenic substrates bearing nitrophenyl groups and do not inhibit the activities of β-galactosidase and alkaline phosphatase, the two enzymes tested thus far. NDSB-195 has the added advantage in that it does not absorb at 280 nm, making it compatible with protein purification procedures in which the protein concentrations are determined by absorbance measurements at 280 nm. Supplied with a data sheet.
FormSolid
SolubilityH₂O (50 mg/ml)
Storage +15°C to +30°C
Hygroscopic
Do Not Freeze Ok to freeze
Special InstructionsStore lyophilized products at room temperature. Following reconstitution, store in the refrigerator (4°C) or at room temperature (20°C).
Toxicity Irritant
ReferencesGoldberg, M.E., et al. 1996. Folding and Design 1, 21.
Goldberg, M.E., et al. 1995. Folding & Design 1, 21.
Vuillard, L., et al. 1995. Biochem. J. 305, 337.
Vuillard, L., et al. 1995. Anal. Biochem. 230, 290.
Vuillard, L., et al. 1994. FEBS Lett. 353, 294.

Produits & Applications associés

Related Products

Référence Description
480001 NDSB-195 - CAS 160255-06-1 - Calbiochem
480005 NDSB-201 - CAS 15471-17-7 - Calbiochem
480010 NDSB-256 - CAS 81239-45-4 - Calbiochem

Related Products

Référence Description
480001 NDSB-195 - CAS 160255-06-1 - Calbiochem
480005 NDSB-201 - CAS 15471-17-7 - Calbiochem
480010 NDSB-256 - CAS 81239-45-4 - Calbiochem

Catégories

Life Science Research > Inhibitors and Biochemicals > Biochemicals > Detergents > Non-Detergent Sulfobetaines