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475911 Myeloperoxidase, Human Polymorphonuclear Leukocytes

A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: MPO

475911
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Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
475911-100UG Ampoule plast. 100 μg
Description
OverviewNative, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.
Catalogue Number475911
Brand Family Calbiochem®
SynonymsMPO
References
ReferencesNauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol. 107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun. 189, 1129.
Product Information
CAS number9003-99-0
Unit of DefinitionOne unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.
EC number1.11.1.7
FormLyophilized
FormulationLyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
Quality LevelMQ200
Applications
Biological Information
Purity≥95% by SDS-PAGE
SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity150-200 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
475911-100UG 04055977203103

Documentation

Myeloperoxidase, Human Polymorphonuclear Leukocytes FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Myeloperoxidase, Human Polymorphonuclear Leukocytes Certificats d'analyse

TitreNuméro de lot
475911

Références bibliographiques

Aperçu de la référence bibliographique
Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol. 107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun. 189, 1129.

Citations

Titre
  • Armin Schnabel, Elena Csernok, Jorg Braun, Wolfgang Gross. (2000) Activation of Neutrophils, Eosinophils, and Lymphocytes in the Lower Respiratory Tract in Wegener's Granulomatosis. American Journal of Critical Care Medicine 161, 399-405.
    		•
    Fiche technique

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision11-August-2008 RFH
    SynonymsMPO
    DescriptionNative, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.
    FormLyophilized
    FormulationLyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.
    SourcePrepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
    CAS number9003-99-0
    EC number1.11.1.7
    Purity≥95% by SDS-PAGE
    Specific activity150-200 units/mg protein
    Unit definitionOne unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.
    SolubilityH₂O (volume is lot-specific and can be found on the product label). Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.
    Storage -20°C
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.
    Toxicity Standard Handling
    ReferencesNauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
    Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
    Mulligan, M.S., et al. 1992. Br. J. Pharmacol. 107, 1159.
    Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun. 189, 1129.
    Citation
  • Armin Schnabel, Elena Csernok, Jorg Braun, Wolfgang Gross. (2000) Activation of Neutrophils, Eosinophils, and Lymphocytes in the Lower Respiratory Tract in Wegener's Granulomatosis. American Journal of Critical Care Medicine 161, 399-405.
    		•