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LSKGEL050 Kit Montage Gel Extraction

DNA Gel Extraction

Kit Montage Gel Extraction : FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
LSKGEL050
50  
Purchase on Sigma-Aldrich

Aperçu

Replacement Information
Description
RéférenceLSKGEL050
Nom de marque
  • Montage
DescriptionKit Montage Gel Extraction
AperçuRecover 100 to 10,000 bp DNA from agarose gel slices in a single 10-minute spin. The kit consists of a pre-assembled filter device with an agarose gel nebulizer, a microcentrifuge vial, and modified TAE gel extraction buffer (dry).

The device utilizes gel compression to extract DNA from the agarose. Centrifugal force collapses the gel structure, drives the agarose through a small orifice in the gel nebulizer and the resultant gel slurry is sprayed into the sample filter cup.

Prepared DNA requires no further purification for most applications, including cloning and radioisotopic or fluorescent DNA sequencing. Since agarose gel electrophoresis has high resolving power, the small and large non-specific amplification products that frequently interfere with cloning and sequencing after PCR are completely removed from the product.

  • Minimal hands-on time
  • 10-minute spin
  • Fully-functional DNA


Performance
Typical DNA Recoveries from Agarose Gels

DNA Size (bp) Percent DNA Recoveries

100 78
700 71
1000 77
2027 47
4361 35
9416 32
23130 29

Protocol
1. Excise the band of interest after routine electrophoretic analysis of PCR products or other DNA through an agarose gel.
2. Place the gel slice into the pre-assembled device. Note: Protocol is not compatible with low melting point agarose.
3. Spin at 5,000 x g for 10 minutes to extract gel slurry into filter cup.
4. DNA can be stored in the capped filtrate vial after discarding the sample filter cup and gel nebulizer.
Alternate Names
  • Ultrafree-DA
Références bibliographiques
Informations produit
Composants
  • comprend 50 dispositifs Ultrafree-DA et 500 ml de solution stock 50x de tampon TAE modifié
Code couleurBleu
Quality LevelMQ300
Applications
ApplicationDNA Gel Extraction
Informations biologiques
Informations physico-chimiques
Dimensions
Informations sur les matériaux
Matériau du dispositif
  • Polypropylene
Informations toxicologiques
Informations de sécurité selon le SGH
Informations sur la sécurité
Notifications sur l'utilisation du produit
Informations relatives au stockage et à l'expédition
Informations sur l'emballage
Quantité50
Informations sur le transport
Information complémentaire
Caractéristiques
Global Trade Item Number
Référence GTIN
LSKGEL050 04053252326288