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FCMAB133F Milli-Mark™ Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate

Detect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.
Research Category: Stem Cell Research Research Sub-Category: Pluripotent & Early Differentiation Gene Symbol: TRA-2-49
FCMAB133F
100 tests  
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Tableau de caractéristiques principal

Species ReactivityKey ApplicationsHostFormatAntibody Type
HFCMFITCMonoclonal Antibody
Description
Catalogue NumberFCMAB133F
Trade Name
  • Milli-Mark
DescriptionMilli-Mark™ Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate
Background InformationIn humans, four isoforms of alkaline phosphatase exist: intestinal, placental, placenta-like (germ cell type) and tissue-nonspecific. The tissue-nonspecific isoform of alkaline phosphatase is present in bone, liver and kidney; it differs from the other forms by posttranslational modification.
Undifferentiated human Embryonal Carcinoma (EC) and Embryonic Stem (ES) cells have been shown to express very high levels of Alkaline Phosphatase isozyme that is indistinguishable from the isozyme found in liver, bone and kidney. Expression levels of AP decrease following stem cell differentiation. This clone can be used to monitor the expression of the Human Liver / Bone / Kidney isozyme of Alkaline Phosphatase (AP), and hence the differentiation status of human EC and ES cells by flow cytometry.
Product Information
FormatFITC
Control
  • 2102 Ep cells.
PresentationPurified mouse monoclonal IgG1 conjugated to FITC in PBS with 0.1% sodium azide and 15 mg/mL BSA.
Quality LevelMQ100
Applications
ApplicationDetect TRA-2-49 using this Milli-Mark Anti-TRA-2-49 Antibody, clone TRA-2-49/6E, FITC Conjugate validated for use in FC.
Key Applications
  • Flow Cytometry
Working Solution Cells / test Total Reaction Volume
5 μl 5 x 105 Cells / 95 μl PBS 100 μl
10 μl 1 x 106 Cells / 90 μl PBS 100 μl
Biological Information
Immunogen2102Ep human Embryonal Carcinoma Cells
EpitopeUnknown
CloneTRA-2-49/6E
HostMouse
SpecificityAntibody recognizes TRA-2-49.
IsotypeIgG1
Species Reactivity
  • Human
Antibody TypeMonoclonal Antibody
Gene Symbol
  • TRA-2-49
Purification MethodProtein A Purfied
Molecular Weight 212/80/55 kDa Observed
Product Usage Statements
Quality AssuranceEvaluated by flow cytometry using 2102 Ep cells.
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsMaintain refrigerated at 2-8°C protected from light in undiluted aliquots for up to 6 months from date of receipt.
Packaging Information
Material Size100 tests
Global Trade Item Number
Référence GTIN
FCMAB133F 04053252509797