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17-610 Magna ChIP™ A - Chromatin Immunoprecipitation Kit

17-610
22 assays  Kit capacity: 22 chromatin immunoprecipitation assays
Purchase on Sigma-Aldrich

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Aperçu

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For target-specific spike-in controls that make ChIP experiments more quantitative and accurate, Click on the Related Product & Applications tab above.
Description
Catalogue Number17-610
Trade Name
  • Magna ChIP
DescriptionMagna ChIP™ A - Chromatin Immunoprecipitation Kit
OverviewChromatin Immunoprecipitation (ChIP) is an important technique allowing the researcher to analyze in vivo interactions of proteins with genomic DNA. Any chromatin-associated or DNA binding protein can be analyzed with this technique, provided a good antibody to the protein exists. One can measure different proteins localized to a specific region of the genome, or the genome wide distribution of a specific protein. Another powerful application of this technique is to analyze changes in histone modifications that correlate with processes like transcription, mitosis or DNA repair.

Features & Benefits:
Faster: Magnetic protein A beads allow for the entire ChIP protocol to be done in as little as a day! All reagents to process your samples are included - you don't have to spend valuable time making them.
Easier: Spin columns make DNA purification easier and more reliable - no more messy phenol-chloroform extractions.
Alternate Names
  • Magnetic ChIP Kit
Materials Required but Not DeliveredMagna Grip™ Rack 8 well (Cat.# 20-400) (Now Available!) or similar magnetic rack.
Background InformationChromatin Immunoprecipitation (ChIP) is a powerful technique for mapping the in vivo distribution of proteins associated with chromosomal DNA. These proteins can be histone subunits and post-translational modifications or other chromatin associated proteins such as transcription factors, chromatin regulators, etc. Additionally, ChIP can be used to identify regions of the genome associated with these proteins, or conversely, to identify proteins associated with a particular region of the genome. ChIP methodology often involves protein-DNA and protein-protein cross-linking, fragmentation of the cross-linked chromatin, and subsequent immunoprecipitation of chromatin with an antibody specific to a target protein. The DNA fragments isolated in complex with the target protein can be identified by a variety of methods including PCR, DNA microarray and DNA sequencing. Standard or quantitative PCR can be performed to verify whether a particular DNA sequence (the gene or region of the genome) is associated with the protein of interest. The combination of ChIP and promoter or genomic tiling microarrays (ChIP-chip) allows genome-wide identification of DNA-binding sites for chromatin-associated proteins with precise resolution. Alternatively, high-throughput sequencing of libraries constructed from immunoprecipitated chromosomal DNA (ChIP-Seq) is a powerful alternative to ChIP-chip in mapping the protein-DNA interactions across mammalian genomes.
References
Product Information
Components
  • Magnetic Protein A Beads
  • ChIP Dilution Buffer
  • Low Salt Wash Buffer
  • High Salt Wash Buffer
  • LiCl Wash Buffer
  • TE Buffer
  • Cell Lysis Buffer
  • Nuclear Lysis Buffer
  • ChIP Elution Buffer (w/o Proteinase K)
  • 10X Glycine
  • 10X PBS
  • Protease Inhibitor Cocktail II
  • Proteinase K
  • Spin Filters
  • Collection Tubes
  • Bind Reagent A
  • Wash Reagent B
  • Elution Reagent C
HS Code3822 19 90
PresentationTwo boxes containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions. Supplied buffers are sufficient to generate chromatin from up to five 15 cm plates of cultured cells, each plate providing up to 10 chromatin preparations (varies with cell and assay type).
Quality LevelMQ100
Applications
ApplicationSingle day chromatin immunoprecipitation (ChIP) kit containing all necessary reagents to perform 22 individual chromatin immunoprecipitation (ChIP) reactions using magnetic A beads.
Biological Information
Analytes Available
  • Protein A
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsUpon receipt, store components at the temperatures indicated on the labels. Kit components are stable for 1 year from date of shipment when stored as directed.
Packaging Information
Material Size22 assays
Material PackageKit capacity: 22 chromatin immunoprecipitation assays
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
17-610 04053252005374

Documentation

Magna ChIP™ A - Chromatin Immunoprecipitation Kit FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

Magna ChIP™ A - Chromatin Immunoprecipitation Kit Certificats d'analyse

TitreNuméro de lot
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 1982642 1982642
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2014533 2014533
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2032316 2032316
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2045692 2045692
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2089008 2089008
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2137004 2137004
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2153036 2153036
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2189711 2189711
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2197231 2197231
Magna ChIP A or EZ-Magna ChIP A Chromatin Immunoprecipitation Kits - 2266497 2266497

Références bibliographiques

Aperçu de la référence bibliographiqueNº PubMed
ANKRD11 associated with intellectual disability and autism regulates dendrite differentiation via the BDNF/TrkB signaling pathway.
Ka, M; Kim, WY
Neurobiol Dis  111  138-152  2018

Afficher le résumé
29274743 29274743
Atrx inactivation drives disease-defining phenotypes in glioma cells of origin through global epigenomic remodeling.
Danussi, C; Bose, P; Parthasarathy, PT; Silberman, PC; Van Arnam, JS; Vitucci, M; Tang, OY; Heguy, A; Wang, Y; Chan, TA; Riggins, GJ; Sulman, EP; Lang, F; Creighton, CJ; Deneen, B; Miller, CR; Picketts, DJ; Kannan, K; Huse, JT
Nat Commun  9  1057  2018

Afficher le résumé
29535300 29535300
Discovery of a Glucocorticoid Receptor (GR) Activity Signature Using Selective GR Antagonism in ER-Negative Breast Cancer.
West, DC; Kocherginsky, M; Tonsing-Carter, EY; Dolcen, DN; Hosfield, DJ; Lastra, RR; Sinnwell, JP; Thompson, KJ; Bowie, KR; Harkless, RV; Skor, MN; Pierce, CF; Styke, SC; Kim, CR; de Wet, L; Greene, GL; Boughey, JC; Goetz, MP; Kalari, KR; Wang, L; Fleming, GF; Győrffy, B; Conzen, SD
Clin Cancer Res  0  2018

Afficher le résumé
29636357 29636357
The long non-coding RNA 91H increases aggressive phenotype of breast cancer cells and up-regulates H19/IGF2 expression through epigenetic modifications.
Vennin, C; Spruyt, N; Robin, YM; Chassat, T; Le Bourhis, X; Adriaenssens, E
Cancer Lett  385  198-206  2016

Afficher le résumé
27780718 27780718
Status of hepatic DNA methylome predetermines and modulates the severity of non-alcoholic fatty liver injury in mice.
Tryndyak, VP; Han, T; Fuscoe, JC; Ross, SA; Beland, FA; Pogribny, IP
BMC Genomics  17  298  2015

Afficher le résumé
27103143 27103143
IL-6/STAT3 axis initiated CAFs via up-regulating TIMP-1 which was attenuated by acetylation of STAT3 induced by PCAF in HCC microenvironment.
Zheng, X; Xu, M; Yao, B; Wang, C; Jia, Y; Liu, Q
Cell Signal  28  1314-24  2015

Afficher le résumé
27297362 27297362
Essential Roles for ARID1B in Dendritic Arborization and Spine Morphology of Developing Pyramidal Neurons.
Ka, M; Chopra, DA; Dravid, SM; Kim, WY
J Neurosci  36  2723-42  2015

Afficher le résumé
26937011 26937011
Investigation of genes important in neurodevelopment disorders in adult human brain.
Maussion, G; Diallo, AB; Gigek, CO; Chen, ES; Crapper, L; Théroux, JF; Chen, GG; Vasuta, C; Ernst, C
Hum Genet  134  1037-53  2015

Afficher le résumé
26194112 26194112
Genistein disrupts glucocorticoid receptor signaling in human uterine endometrial Ishikawa cells.
Whirledge, S; Senbanjo, LT; Cidlowski, JA
Environ Health Perspect  123  80-7  2015

Afficher le résumé
25136773 25136773
FOXO1-mediated epigenetic modifications are involved in the insulin-mediated repression of hepatocyte aquaporin 9 expression.
Qiu, LW; Gu, LY; Lü, L; Chen, XF; Li, CF; Mei, ZC
Mol Med Rep  11  3064-8  2015

Afficher le résumé
25503267 25503267

Brochure

Titre
Shaping Epigenetics Discovery - Epigenetics Product Selection Brochure

Informations techniques

Titre
White Paper - The Message in the Marks: Deciphering Cancer Epigenetics

Fiche technique

Titre
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

FAQ

QuestionRéponse
How many PCR reactions can be done with this kit?There are enough primers and PCR buffer for 4 reactions per IP assuming a 20 microliter volume and assuming the primers are at the recommended concentration as stated in the manual.
From where are the primer sequences derived for the kit?The primer sequences are based on the Human GAPDH promoter. The GenBank number is NT_009759.15, using nts:6497145-6498136.

Manuels d'utilisation

Titre
Magna ChIP™ A

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Catégories

Life Science Research > Antibodies and Assays > Immunoassays > Immunoprecipitation (IP) > Chromatin Immunoprecipitation (ChIP) > ChIP Kits & Beads
Life Science Research > Kits & Assays > ChIP Kits > Kits and Assays