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475935 MT1-MMP, Catalytic Domain, Human, Recombinant, E. coli

FDS (Fiches de données de sécurité), certificats d’analyse (CoA) et de qualité (CoQ), dossiers, brochures et autres documents disponibles.

Synonymes: MMP-14 Catalytic Domain

475935
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Aperçu

Replacement Information

Products

RéférenceConditionnement Qté
475935-10UG Ampoule plast. 10 μg
Description
OverviewRecombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). Degrades fibrillar collagen Types I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13 resulting in enhanced proteolysis of the matrix for cell migration and proliferation.
Note: 1 mU = 1 milliunit.
Catalogue Number475935
Brand Family Calbiochem®
SynonymsMMP-14 Catalytic Domain
References
ReferencesAmano, T., et al. 2005. Cell Res. 15, 150.
Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
Sato, H., et al. 1994. Nature 370, 61.
Knight, C.G., et al. 1992. REBS Lett. 296, 263.
Product Information
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (<a href ="/Products/ProductDisplay.asp→catNO=03-32-5032">Cat. No. 03-32-5032</a>) per min at 37°C, pH 7.5.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥140 mU/mg protein
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
475935-10UG 04055977184235

Documentation

MT1-MMP, Catalytic Domain, Human, Recombinant, E. coli FDS

Titre

Fiche de données de sécurité des matériaux (FDS) 

MT1-MMP, Catalytic Domain, Human, Recombinant, E. coli Certificats d'analyse

TitreNuméro de lot
475935

Références bibliographiques

Aperçu de la référence bibliographique
Amano, T., et al. 2005. Cell Res. 15, 150.
Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
Sato, H., et al. 1994. Nature 370, 61.
Knight, C.G., et al. 1992. REBS Lett. 296, 263.
Fiche technique

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision17-September-2007 RFH
SynonymsMMP-14 Catalytic Domain
DescriptionRecombinant, human pro-MT1-MMP expressed in E. coli and activated to yield the active catalytic domain (amino acids 89-265). The catalytic domain is comprised of amino acid residues Tyr89 to Gly265 of mature human MT1-MMP. Hydrolyzes fibrillar collagens I, II, and III, as well as other extracellular matrix proteins, and activates MMP-2 and MMP-13, resulting in enhanced proteolysis of the matrix for cell migration and proliferation.
FormLiquid
FormulationIn 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl₂, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Recommended reaction conditions
Activity Assay
Reagent Preparation and Required Equipment Peptide Hydrolysis Buffer: 150 mM NaCl, 50 mM Tris-HCl, 5 mM CaCl2, 0.025 % Brij® 35 detergent, pH 7.5. Store at 4°C; stable for several weeks. Peptide Substrate Stock: 100 µM Mca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg (Cat. No. 05-32-5032) in 20% DMSO. Store at -20°C. Peptide Standard: 10 µM (7-methoxycoumarin-4-yl)acetyl-Pro-Leu-NH2 (Mca-Pro-Leu) (Cat. No. 05-32-5033) in 20% DMSO. Store at -20°C. Fluorimeter: Excitation wavelength: ~328 nm; emission wavelength: ~393 nm
Protocol Note: This protocol is provided only as a guide; assay conditions should be optimized for individual experiments.
The protocol is based on the following reference: Knight, C.G., et al. 1992. FEBS Lett. 296, 263.
1. Calibrate the fluorimeter using the Peptide Standard (Mca-Pro-Leu) at a concentration that corresponds to 2–10% hydrolysis of the Peptide Substrate. 2. Kinetic Dilute the Peptide Substrate Stock to 8 µM in Peptide Hydrolysis Buffer and equilibrate to 37°C. 3. Add 2–4 µl MT1-MMP and record the increase in fluorescence at desired intervals from 2–12 min. 4. Calculate activity in units per ml enzyme using the following equation: Activity (U/ml) = cMca-Pro-Leu/FMca-Pro-Leu x ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg/venzyme x Vtotal cMca-Pro-Leu: Concentration of Mca-Pro-Leu used for calibration of the fluorimeter (µmol/ml) FMca-Pro-Leu: Fluorescence of Mca-Pro-Leu at the concentration cMca-Pro-Leu used for fluorimeter calibration ΔFMca-Pro-Leu-Gly-Leu-Dpa-Ala-Arg: Change in fluorescence during peptide hydrolysis per min V: Volume of peptide hydrolysis reaction (2.5 ml) v: Volume of added enzyme (2–4 µl)
Purity≥90% by SDS-PAGE
Specific activity≥140 mU/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol MCA-Pro-Leu-Gly-Leu-Dpa-Ala-Arg-NH₂ (Cat. No. 03-32-5032) per min at 37°C, pH 7.5.
Storage Avoid freeze/thaw
≤ -70°C
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesAmano, T., et al. 2005. Cell Res. 15, 150.
Knauper, V., et al. 1996. J. Biol. Chem. 271, 17124.
Pei, D., and Weiss, S.J. 1996. J. Biol. Chem. 271, 9135.
Sato, H., et al. 1994. Nature 370, 61.
Knight, C.G., et al. 1992. REBS Lett. 296, 263.