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14-624M MST1 Protein, active, 250 µg

Active, N-terminal His6-tagged, recombinant, full-length, human MST1, for use in Kinase Assays.

14-624M
250 µg  Also available in 10 μg size (cat#14-624) and in bulk (cat#14-624-K).
Purchase on Sigma-Aldrich

Aperçu

Replacement Information
Description
Catalogue Number14-624M
Brand Family Upstate
Trade Name
  • Upstate
DescriptionMST1 Protein, active, 250 µg
OverviewN-terminal His6-tagged, recombinant, full-length, human MST1
References
Product Information
Quality LevelMQ300
Applications
ApplicationActive, N-terminal His6-tagged, recombinant, full-length, human MST1, for use in Kinase Assays.
Key Applications
  • Kinase Assay
Biological Information
Sourcebaculovirus in Sf21 insect cells
Entrez Gene Number
Entrez Gene SummaryThe protein encoded by this gene is a cytoplasmic kinase that is structurally similar to the yeast Ste20p kinase, which acts upstream of the stress-induced mitogen-activated protein kinase cascade. The encoded protein can phosphorylate myelin basic protein and undergoes autophosphorylation. A caspase-cleaved fragment of the encoded protein has been shown to be capable of phosphorylating histone H2B. The particular phosphorylation catalyzed by this protein has been correlated with apoptosis, and it's possible that this protein induces the chromatin condensation observed in this process.
Gene Symbol
  • STK4
  • DKFZp686A2068
  • MST-1
  • MST1
  • KRS2
  • YSK3
Protein TargetMST1
Purification MethodNi2+/NTA-agarose
Target Sub-FamilySTE
UniProt Number
UniProt SummaryFUNCTION: SwissProt: Q13043 # Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Phosphorylates 'Ser-14' of histone H2B during apoptosis. Phosphorylates FOXO3 upon oxidative stress, which results in its nuclear translocation and cell death initiation.
COFACTOR: Magnesium.
SIZE: 487 amino acids; 55630 Da
SUBUNIT: Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation. Interacts with and stabilizes SAV1. Interacts with RASSF1, which leads to enzyme activation. Interacts with FOXO3.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=The caspase-cleaved form cycles between the nucleus and cytoplasm.
TISSUE SPECIFICITY: Ubiquitously expressed.
PTM: Autophosphorylated on serine and threonine residues.
SIMILARITY: SwissProt: Q13043 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain. & Contains 1 SARAH domain.
Molecular Weight57kDa
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Quality AssuranceRoutinely evaluated by phosphorylation of 250μM Axltide (KKSRGDYMTMQIG)
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions6 months at -70°C
Packaging Information
Material Size250 µg
Material PackageAlso available in 10 μg size (cat#14-624) and in bulk (cat#14-624-K).
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Référence GTIN
14-624M 04053252824661

Documentation

Produits & Applications associés

Alternative Packsize

Référence Description
14-624 MST1 Protein, active, 10 µg

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Catégories

Life Science Research > Proteins and Enzymes > Purified Kinases
Life Science Research > Drug Discovery and Development > Kinase & Phosphatase Screening > Purified Kinases